{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T00:06:30Z","timestamp":1767917190292,"version":"3.49.0"},"reference-count":25,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2024,3,31]],"date-time":"2024-03-31T00:00:00Z","timestamp":1711843200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Jiangsu Province for College Students\u2019 Innovation and Entrepreneurship","award":["202310313046Z"],"award-info":[{"award-number":["202310313046Z"]}]},{"name":"Jiangsu Province for College Students\u2019 Innovation and Entrepreneurship","award":["KC22080"],"award-info":[{"award-number":["KC22080"]}]},{"name":"Jiangsu Province for College Students\u2019 Innovation and Entrepreneurship","award":["SISPM-2022-04"],"award-info":[{"award-number":["SISPM-2022-04"]}]},{"name":"Key R&amp;D projects of Xuzhou","award":["202310313046Z"],"award-info":[{"award-number":["202310313046Z"]}]},{"name":"Key R&amp;D projects of Xuzhou","award":["KC22080"],"award-info":[{"award-number":["KC22080"]}]},{"name":"Key R&amp;D projects of Xuzhou","award":["SISPM-2022-04"],"award-info":[{"award-number":["SISPM-2022-04"]}]},{"name":"Key Laboratory of Surface &amp; Interface Science of Polymer Materials of Zhejiang Province","award":["202310313046Z"],"award-info":[{"award-number":["202310313046Z"]}]},{"name":"Key Laboratory of Surface &amp; Interface Science of Polymer Materials of Zhejiang Province","award":["KC22080"],"award-info":[{"award-number":["KC22080"]}]},{"name":"Key Laboratory of Surface &amp; Interface Science of Polymer Materials of Zhejiang Province","award":["SISPM-2022-04"],"award-info":[{"award-number":["SISPM-2022-04"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>This research centers on the development and synthesis of a longwave fluorescence probe, labeled as 60T, designed for the simultaneous detection of hydrogen sulfide, cysteine\/homocysteine, and glutathione. The probe showcases a swift response, good linearity range, and heightened sensitivity, boasting that the detection limits of the probe for Cys, Hcy, GSH and H2S were 0.140, 0.202, 0.259 and 0.396 \u03bcM, respectively. Notably, its efficacy in monitoring thiol status changes in live MCF-7 cells is underscored by a substantial decrease in fluorescence intensity upon exposure to the thiol trapping reagent, N-ethyl maleimide (NEM). With an impressive red emission signal at 630 nm and a substantial Stokes shift of 80 nm, this probe exhibits remarkable sensitivity and selectivity for biothiols and H2S, indicating promising applications in the diagnosis and surgical navigation of relevant cancers.<\/jats:p>","DOI":"10.3390\/molecules29071572","type":"journal-article","created":{"date-parts":[[2024,3,31]],"date-time":"2024-03-31T13:32:56Z","timestamp":1711891976000},"page":"1572","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["A Red-Emission Fluorescent Probe for Intracellular Biothiols and Hydrogen Sulfide Imaging in Living Cells"],"prefix":"10.3390","volume":"29","author":[{"given":"Yuanfan","family":"Wang","sequence":"first","affiliation":[{"name":"The First Clinical Medical College, Xuzhou Medical University, Xuzhou 221004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shengxiang","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Xuzhou Medical University, Xuzhou 221004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tianle","family":"Liu","sequence":"additional","affiliation":[{"name":"The First Clinical Medical College, Xuzhou Medical University, Xuzhou 221004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junning","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Xuzhou Medical University, Xuzhou 221004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bingrui","family":"Yuan","sequence":"additional","affiliation":[{"name":"The First Clinical Medical College, Xuzhou Medical University, Xuzhou 221004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cuntao","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Breast Surgery, Xuzhou Central Hospital, Xuzhou 221004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiumei","family":"Bo","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences, Xuzhou Medical University, Xuzhou 221004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhou","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Xuzhou Medical University, Xuzhou 221004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,3,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e13065","DOI":"10.1097\/MD.0000000000013065","article-title":"The biologic effect of hydrogen sulfide and its function in various diseases","volume":"97","author":"Xiao","year":"2018","journal-title":"Medicine"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3365","DOI":"10.1021\/acssensors.0c02005","article-title":"Sensing and bioimaging of the gaseous signaling molecule hydrogen sulfide by near-infrared fluorescent probes","volume":"5","author":"Jose","year":"2020","journal-title":"ACS Sens."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"77","DOI":"10.2174\/187152811794776277","article-title":"Hydrogen sulfide: From physiology to pharmacology","volume":"10","author":"Fiorucci","year":"2011","journal-title":"Inflamm. 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Soc."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"790","DOI":"10.1038\/nature09472","article-title":"Quantitative reactivity profiling predicts functional cysteines in proteomes","volume":"468","author":"Weerapana","year":"2010","journal-title":"Nature"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1080\/13813450701661198","article-title":"The central role of glutathione in the pathophysiology of human diseases","volume":"113","author":"Franco","year":"2007","journal-title":"Arch. Physiol. Biochem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1038\/nn1609","article-title":"Neuronal glutathione deficiency and age-dependent neurodegeneration in the EAAC1 deficient mouse","volume":"9","author":"Aoyama","year":"2006","journal-title":"Nat. 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