{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T15:13:39Z","timestamp":1788966819482,"version":"build-2803163510"},"reference-count":48,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,4,10]],"date-time":"2024-04-10T00:00:00Z","timestamp":1712707200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["82305086"],"award-info":[{"award-number":["82305086"]}]},{"name":"National Natural Science Foundation of China","award":["2021JQ-745"],"award-info":[{"award-number":["2021JQ-745"]}]},{"name":"National Natural Science Foundation of China","award":["023-ZQNG-001"],"award-info":[{"award-number":["023-ZQNG-001"]}]},{"name":"National Natural Science Foundation of China","award":["L2022CXNLRC010"],"award-info":[{"award-number":["L2022CXNLRC010"]}]},{"name":"Shaanxi Provincial Natural Science Basic Research Program Project","award":["82305086"],"award-info":[{"award-number":["82305086"]}]},{"name":"Shaanxi Provincial Natural Science Basic Research Program Project","award":["2021JQ-745"],"award-info":[{"award-number":["2021JQ-745"]}]},{"name":"Shaanxi Provincial Natural Science Basic Research Program Project","award":["023-ZQNG-001"],"award-info":[{"award-number":["023-ZQNG-001"]}]},{"name":"Shaanxi Provincial Natural Science Basic Research Program Project","award":["L2022CXNLRC010"],"award-info":[{"award-number":["L2022CXNLRC010"]}]},{"name":"Shaanxi Province Administration of Traditional Chinese Medicine Provincial Young and Middle-aged Science and Technology Backbone Project","award":["82305086"],"award-info":[{"award-number":["82305086"]}]},{"name":"Shaanxi Province Administration of Traditional Chinese Medicine Provincial Young and Middle-aged Science and Technology Backbone Project","award":["2021JQ-745"],"award-info":[{"award-number":["2021JQ-745"]}]},{"name":"Shaanxi Province Administration of Traditional Chinese Medicine Provincial Young and Middle-aged Science and Technology Backbone Project","award":["023-ZQNG-001"],"award-info":[{"award-number":["023-ZQNG-001"]}]},{"name":"Shaanxi Province Administration of Traditional Chinese Medicine Provincial Young and Middle-aged Science and Technology Backbone Project","award":["L2022CXNLRC010"],"award-info":[{"award-number":["L2022CXNLRC010"]}]},{"name":"Xianyang City Innovation Capability Service Support Plan\u2014Science and Technology Leading Talents","award":["82305086"],"award-info":[{"award-number":["82305086"]}]},{"name":"Xianyang City Innovation Capability Service Support Plan\u2014Science and Technology Leading Talents","award":["2021JQ-745"],"award-info":[{"award-number":["2021JQ-745"]}]},{"name":"Xianyang City Innovation Capability Service Support Plan\u2014Science and Technology Leading Talents","award":["023-ZQNG-001"],"award-info":[{"award-number":["023-ZQNG-001"]}]},{"name":"Xianyang City Innovation Capability Service Support Plan\u2014Science and Technology Leading Talents","award":["L2022CXNLRC010"],"award-info":[{"award-number":["L2022CXNLRC010"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Traditional Chinese medicine (TCM) possesses the potential of providing good curative effects with no side effects for the effective management of slow transit constipation (STC), an intestinal disease characterized by colonic dyskinesia. Mulberry leaves (Morus alba L.) and black sesame (Sesamum indicum L.), referred to as SH, are processed and conditioned as per standardized protocols. SH has applications as food and medicine. Accordingly, we investigated the therapeutic potential of SH in alleviating STC. The analysis of SH composition identified a total of 504 compounds. The intervention with SH significantly improved intestinal motility, reduced the time for the first black stool, increased antioxidant activity, and enhanced water content, thereby effectively alleviating colon damage caused by STC. Transcriptome analysis revealed the SH in the treatment of STC related to SOD1, MUC2, and AQP1. The analysis of 16S rRNA gene sequences indicated notable differences in the abundance of 10 bacteria between the SH and model. Metabolomic analysis further revealed that SH supplementation increased the levels of nine metabolites associated with STC. Integrative analysis revealed that SH modulated amino acid metabolism, balanced intestinal flora, and targeted key genes (i.e., SOD1, MUC2, AQP1) to exert its effects. SH also inhibited the AQP1 expression and promoted SOD1 and MUC2 expression.<\/jats:p>","DOI":"10.3390\/molecules29081713","type":"journal-article","created":{"date-parts":[[2024,4,10]],"date-time":"2024-04-10T10:55:28Z","timestamp":1712746528000},"page":"1713","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Mechanism of Mulberry Leaves and Black Sesame in Alleviating Slow Transit Constipation Revealed by Multi-Omics Analysis"],"prefix":"10.3390","volume":"29","author":[{"given":"Chen","family":"Sun","sequence":"first","affiliation":[{"name":"College of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China"},{"name":"Key Laboratory of Modern Research of TCM, Education Department of Hunan Province, Hunan University of Chinese Medicine, Changsha 410208, China"},{"name":"Co-Construction Collaborative Innovation Center for Chinese Medicine Resources Industrialization by Shaanxi & Education Ministry, Shaanxi University of Chinese Medicine, Xianyang 712046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Wang","sequence":"additional","affiliation":[{"name":"Co-Construction Collaborative Innovation Center for Chinese Medicine Resources Industrialization by Shaanxi & Education Ministry, Shaanxi University of Chinese Medicine, Xianyang 712046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4834-6292","authenticated-orcid":false,"given":"Yang","family":"Tan","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China"},{"name":"National Key Laboratory Cultivation Base of Chinese Medicinal Powder & Innovative Medicinal Jointly Established by Province and Ministry, Hunan University of Chinese Medicine, Changsha 410208, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Li","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China"},{"name":"Key Laboratory of Modern Research of TCM, Education Department of Hunan Province, Hunan University of Chinese Medicine, Changsha 410208, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Zhou","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China"},{"name":"Key Laboratory of Modern Research of TCM, Education Department of Hunan Province, Hunan University of Chinese Medicine, Changsha 410208, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shi-An","family":"Hu","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China"},{"name":"Key Laboratory of Modern Research of TCM, Education Department of Hunan Province, Hunan University of Chinese Medicine, Changsha 410208, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qin-Wen","family":"Yan","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China"},{"name":"Key Laboratory of Modern Research of TCM, Education Department of Hunan Province, Hunan University of Chinese Medicine, Changsha 410208, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin-Hui","family":"Li","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China"},{"name":"Key Laboratory of Modern Research of TCM, Education Department of Hunan Province, Hunan University of Chinese Medicine, Changsha 410208, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Pei","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China"},{"name":"Key Laboratory of Modern Research of TCM, Education Department of Hunan Province, Hunan University of Chinese Medicine, Changsha 410208, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,10]]},"reference":[{"key":"ref_1","first-page":"5641","article-title":"Intervention effect of benzoylmesaconine on slow transit constipation in rats","volume":"10","author":"Zhang","year":"2023","journal-title":"J. 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