{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T03:20:37Z","timestamp":1790306437648,"version":"4.1.0"},"reference-count":58,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,4,13]],"date-time":"2024-04-13T00:00:00Z","timestamp":1712966400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Beijing University of Chemical Technology\u2014China\u2013Japan Friendship Hospital Biomedical Transformation Engineering Research Center Joint Project","award":["RZ2020-02"],"award-info":[{"award-number":["RZ2020-02"]}]},{"name":"Beijing University of Chemical Technology\u2014China\u2013Japan Friendship Hospital Biomedical Transformation Engineering Research Center Joint Project","award":["2016YFF0203703"],"award-info":[{"award-number":["2016YFF0203703"]}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["RZ2020-02"],"award-info":[{"award-number":["RZ2020-02"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2016YFF0203703"],"award-info":[{"award-number":["2016YFF0203703"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The COVID-19 pandemic over recent years has shown a great need for the rapid, low-cost, and on-site detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this study, an aptamer-based colloidal gold nanoparticle lateral flow test strip was well developed to realize the visual detection of wild-type SARS-CoV-2 spike proteins (SPs) and multiple variants. Under the optimal reaction conditions, a low detection limit of SARS-CoV-2 S proteins of 0.68 nM was acquired, and the actual detection recovery was 83.3% to 108.8% for real-world samples. This suggests a potential tool for the prompt detection of SARS-CoV-2 with good sensitivity and accuracy, and a new method for the development of alternative antibody test strips for the detection of other viral targets.<\/jats:p>","DOI":"10.3390\/molecules29081776","type":"journal-article","created":{"date-parts":[[2024,4,15]],"date-time":"2024-04-15T03:56:13Z","timestamp":1713153373000},"page":"1776","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["The Development of Aptamer-Based Gold Nanoparticle Lateral Flow Test Strips for the Detection of SARS-CoV-2 S Proteins on the Surface of Cold-Chain Food Packaging"],"prefix":"10.3390","volume":"29","author":[{"given":"Xiaotong","family":"Li","sequence":"first","affiliation":[{"name":"Laboratory of Food Safety and Risk Assessment, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiachen","family":"Wang","sequence":"additional","affiliation":[{"name":"Laboratory of Food Safety and Risk Assessment, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ge","family":"Yang","sequence":"additional","affiliation":[{"name":"CAMS Key Laboratory of Antiviral Drug Research, Beijing Key Laboratory of Antimicrobial Agents, NHC Key Laboratory of Biotechnology of Antibiotics, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2545-6509","authenticated-orcid":false,"given":"Xiaona","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Basic Medicine, Anhui Medical College, Hefei 230601, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lianhui","family":"Zhao","sequence":"additional","affiliation":[{"name":"Laboratory of Food Safety and Risk Assessment, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaofeng","family":"Luo","sequence":"additional","affiliation":[{"name":"Key Laboratory of Zhejiang Province for Aptamers and Theragnostic, Aptamer Selection Center, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou 310022, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1752-3935","authenticated-orcid":false,"given":"Yiyang","family":"Dong","sequence":"additional","affiliation":[{"name":"Laboratory of Food Safety and Risk Assessment, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1038\/s41586-020-2008-3","article-title":"A new coronavirus associated with human respiratory disease in China","volume":"579","author":"Wu","year":"2020","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"5822","DOI":"10.3748\/wjg.v27.i35.5822","article-title":"Environmental perspectives of COVID-19 outbreaks: A review","volume":"27","author":"Samanta","year":"2021","journal-title":"World J. 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