{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T19:32:33Z","timestamp":1787167953405,"version":"build-2736575974"},"reference-count":22,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,4,18]],"date-time":"2024-04-18T00:00:00Z","timestamp":1713398400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.mdpi.com"],"crossmark-restriction":true},"short-container-title":["Molecules"],"abstract":"<jats:p>In the search for new bioactive agents against the infectious pathogen responsible for the neglected tropical disease (NTD) mycetoma, we tested a collection of 27 essential oils (EOs) in vitro against Madurella mycetomatis, the primary pathogen responsible for the fungal form of mycetoma, termed eumycetoma. Among this series, the EO of Santalum album (Santalaceae), i.e., East Indian sandalwood oil, stood out prominently with the most potent inhibition in vitro. We, therefore, directed our research toward 15 EOs of Santalum species of different geographical origins, along with two samples of EOs from other plant species often commercialized as \u201csandalwood oils\u201d. Most of these EOs displayed similar strong activity against M. mycetomatis in vitro. All tested oils were thoroughly analyzed by GC-QTOF MS and most of their constituents were identified. Separation of the sandalwood oil into the fractions of sesquiterpene hydrocarbons and alcohols showed that its activity is associated with the sesquiterpene alcohols. The major constituents, the sesquiterpene alcohols (Z)-\u03b1- and (Z)-\u03b2-santalol were isolated from the S. album oil by column chromatography on AgNO3-coated silica. They were tested as isolated compounds against the fungus, and (Z)-\u03b1-santalol was about two times more active than the \u03b2-isomer.<\/jats:p>","DOI":"10.3390\/molecules29081846","type":"journal-article","created":{"date-parts":[[2024,4,18]],"date-time":"2024-04-18T07:55:54Z","timestamp":1713426954000},"page":"1846","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Sandalwood Oils of Different Origins Are Active In Vitro against Madurella mycetomatis, the Major Fungal Pathogen Responsible for Eumycetoma"],"prefix":"10.3390","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9059-6909","authenticated-orcid":false,"given":"Shereen O.","family":"Abd Algaffar","sequence":"first","affiliation":[{"name":"Faculty of Pharmacy, University of Science and Technology, Omdurman 14411, Sudan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephan","family":"Seegers","sequence":"additional","affiliation":[{"name":"University of M\u00fcnster, Institute of Pharmaceutical Biology and Phytochemistry (IPBP), PharmaCampus-Corrensstrasse 48, D-48149 M\u00fcnster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2950-1074","authenticated-orcid":false,"given":"Prabodh","family":"Satyal","sequence":"additional","affiliation":[{"name":"Essential Oil Science, d\u014dTERRA International, 1248 W 700 S, Pleasant Grove, UT 84062, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3639-0528","authenticated-orcid":false,"given":"William N.","family":"Setzer","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Alabama in Huntsville, Huntsville, AL 35899, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas J.","family":"Schmidt","sequence":"additional","affiliation":[{"name":"University of M\u00fcnster, Institute of Pharmaceutical Biology and Phytochemistry (IPBP), PharmaCampus-Corrensstrasse 48, D-48149 M\u00fcnster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sami A.","family":"Khalid","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, University of Science and Technology, Omdurman 14411, Sudan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,18]]},"reference":[{"key":"ref_1","unstructured":"(2024, January 10). World Health Organization\u2013Mycetoma. Available online: https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/mycetoma."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/S1473-3099(15)00359-X","article-title":"Mycetoma: A unique neglected tropical disease","volume":"16","author":"Zijlstra","year":"2016","journal-title":"Lancet Infect. Dis."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Emery, D., and Denning, D.W. (2020). The global distribution of actinomycetoma and eumycetoma. PLOS Negl. Trop. Dis., 14.","DOI":"10.1371\/journal.pntd.0008397"},{"key":"ref_4","unstructured":"(2024, January 30). Drugs for Neglected Diseases Initiative (DNDi). Available online: https:\/\/dndi.org\/press-releases\/2023\/worlds-first-clinical-trial-for-mycetoma-shows-efficacy-new-promising-treatment."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"936","DOI":"10.1093\/jac\/dkz529","article-title":"Madurella mycetomatis, the main causative agent of eumycetoma, is highly susceptible to olorofim","volume":"75","author":"Lim","year":"2020","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1093\/jac\/dkl526","article-title":"In vitro susceptibility of Madurella mycetomatis, prime agent of Madura foot, to tea tree oil and artemisinin","volume":"59","author":"Fahal","year":"2007","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Konings, M., Eadie, K., Lim, W., Fahal, A.H., Mouton, J., Tesse, N., and van de Sande, W.W.J. (2021). The synthetic synergistic cinnamon oil CIN-102 is active against Madurella mycetomatis, the most common causative agent of mycetoma. PLoS Negl. Trop. Dis., 15.","DOI":"10.1371\/journal.pntd.0009488"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Kim, T.H., Hatano, T., Okamoto, K., Yoshida, T., Kanzaki, H., Arita, M., and Ito, H. (2017). Antifungal and Ichthyotoxic Sesquiterpenoids from Santalum album Heartwood. Molecules, 22.","DOI":"10.3390\/molecules22071139"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Powers, C.N., Osier, J.L., McFeeters, R.L., Brazell, C.B., Olsen, E.L., Moriarity, D.M., Satyal, P., and Setzer, W.N. (2018). Antifungal and Cytotoxic Activities of Sixty Commercially-Available Essential Oils. Molecules, 23.","DOI":"10.3390\/molecules23071549"},{"key":"ref_10","unstructured":"(2017). NIST 17 Library & Search Program."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1080\/10412905.2003.9712106","article-title":"Western Australian Sandalwood Oil\u2014New Constituents of Santalum spicatum (R. Br.) A. DC. (Santalaceae)","volume":"3","author":"Valder","year":"2003","journal-title":"J. Essent. Oil Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1002\/ffj.2025","article-title":"Phytochemistry of the heartwood from fragrant Santalum species: A review","volume":"1","author":"Baldovini","year":"2011","journal-title":"Flavour Fragr. J."},{"key":"ref_13","first-page":"1365","article-title":"Hawaiian Sandalwood: Oil Composition of Santalum paniculatum and Comparison with Other Sandal Species","volume":"9","author":"Braun","year":"2014","journal-title":"Nat. Prod. Commun."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Diaz-Chavez, M.L., Moniodis, J., Madilao, L.L., Jancsik, S., Keeling, C.I., Barbour, E.L., Ghisalberti, E.L., Plummer, J.A., Jones, C.G., and Bohlmann, J. (2013). Biosynthesis of Sandalwood Oil: Santalum album CYP76F cytochromes P450 produce santalols and bergamotol. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0075053"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/j.chroma.2003.11.093","article-title":"Evaluation of the quality of sandalwood essential oils by gas chromatography\u2013mass spectrometry","volume":"1028","author":"Howes","year":"2004","journal-title":"J. Chromatogr. A"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Ka\u010d\u00e1niov\u00e1, M., Terentjeva, M., \u0160tef\u00e1nikov\u00e1, J., \u017diarovsk\u00e1, J., Savitskaya, T., Grinshpan, D., Kowalczewski, P.\u0141., Vukovic, N., and Tvrd\u00e1, E. (2020). Chemical Composition and Antimicrobial Activity of Selected Essential Oils against Staphylococcus spp. Isolated from Human Semen. Antibiotics, 9.","DOI":"10.3390\/antibiotics9110765"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1115","DOI":"10.1021\/jf60178a031","article-title":"Structure of Brachyl Oxide","volume":"19","author":"Klein","year":"1971","journal-title":"J. Agric. Food Chem."},{"key":"ref_18","unstructured":"(2024, January 17). BASF\/Isobionics\u00aeSantalol Safety Data Sheet. Available online: https:\/\/products.basf.com\/global\/en\/downloads.html#%7B%220%22%3A%5B%5B%22productId%22%2C%5B%2230794235%22%5D%5D%5D%7D."},{"key":"ref_19","unstructured":"(2024, January 17). Isobionics\u00aeSantalol Product Specification. Available online: https:\/\/www.isobionics.com\/global\/en\/product-finder\/santalol.html."},{"key":"ref_20","unstructured":"Adams, R.P. (2007). Identification of Essential Oil Components by Gas Chromatography\/Mass Spectrometry, Allured Publishing Corporation. [4th ed.]."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"4564","DOI":"10.1039\/c2an35575b","article-title":"Preparative separation of \u03b1- and \u03b2-santalenes and (Z)-\u03b1- and (Z)-\u03b2-santalols using silver nitrate-impregnated silica gel medium pressure liquid chromatography and analysis of sandalwood oil","volume":"19","author":"Daramwar","year":"2012","journal-title":"Analyst"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"e01338-20","DOI":"10.1128\/AAC.01338-20","article-title":"Development and validation of an in vitro resazurin-based susceptibility assay against Madurella mycetomatis","volume":"65","author":"Verbon","year":"2021","journal-title":"Antimicrob. Agents Chemother."}],"updated-by":[{"DOI":"10.3390\/molecules30132763","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2024,4,18]],"date-time":"2024-04-18T00:00:00Z","timestamp":1713398400000}}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/29\/8\/1846\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,3]],"date-time":"2025-08-03T14:29:25Z","timestamp":1754231365000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/29\/8\/1846"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4,18]]},"references-count":22,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2024,4]]}},"alternative-id":["molecules29081846"],"URL":"https:\/\/doi.org\/10.3390\/molecules29081846","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4,18]]}}}