{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:49:09Z","timestamp":1789519749198,"version":"build-2803163510"},"reference-count":50,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2024,5,18]],"date-time":"2024-05-18T00:00:00Z","timestamp":1715990400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["22178174"],"award-info":[{"award-number":["22178174"]}]},{"name":"National Natural Science Foundation of China","award":["XTC2206"],"award-info":[{"award-number":["XTC2206"]}]},{"DOI":"10.13039\/501100013140","name":"Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture","doi-asserted-by":"publisher","award":["22178174"],"award-info":[{"award-number":["22178174"]}],"id":[{"id":"10.13039\/501100013140","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013140","name":"Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture","doi-asserted-by":"publisher","award":["XTC2206"],"award-info":[{"award-number":["XTC2206"]}],"id":[{"id":"10.13039\/501100013140","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The development of immobilized enzymes with high activity and stability is critical. Metal\u2013organic frameworks (MOFs) have attracted much academic and industrial interest in the field of enzyme immobilization due to their unique properties. In this study, the amino-functionalized ionic liquid (NIL)-modified metal\u2013organic framework (UiO-66-NH2) was prepared to immobilize Candida rugosa lipase (CRL), using dialdehyde starch (DAS) as the cross-linker. The results of the Fourier transform infrared (FT-IR) spectra, X-ray powder diffraction (XRD), and scanning electronic microscopy (SEM) confirmed that the NIL was successfully grafted to UiO-66-NH2. The CRL immobilized on NIL-modified UiO-66-NH2 (UiO-66-NH2-NIL-DAS@CRL) exhibited satisfactory activity recovery (79.33%), stability, reusability, and excellent organic solvent tolerance. The research results indicated that ionic liquid-modified UiO-66-NH2 had practical potential for application in enzyme immobilization.<\/jats:p>","DOI":"10.3390\/molecules29102381","type":"journal-article","created":{"date-parts":[[2024,5,20]],"date-time":"2024-05-20T03:36:42Z","timestamp":1716176202000},"page":"2381","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Functionalized Ionic Liquids-Modified Metal\u2013Organic Framework Material Boosted the Enzymatic Performance of Lipase"],"prefix":"10.3390","volume":"29","author":[{"given":"Liran","family":"Ji","sequence":"first","affiliation":[{"name":"State Key Laboratory of Materials-Oriented Chemical Engineering, School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing 210009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Materials-Oriented Chemical Engineering, School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing 210009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifei","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Materials-Oriented Chemical Engineering, School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing 210009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7736-0889","authenticated-orcid":false,"given":"Binbin","family":"Nian","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Materials-Oriented Chemical Engineering, School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing 210009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Hu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Materials-Oriented Chemical Engineering, School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing 210009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,5,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1016\/j.trac.2018.03.011","article-title":"Advances on methods and easy separated support materials for enzymes immobilization","volume":"102","author":"Liu","year":"2018","journal-title":"Trac-Trend Anal. 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