{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:09:21Z","timestamp":1760144961100,"version":"build-2065373602"},"reference-count":28,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2024,6,4]],"date-time":"2024-06-04T00:00:00Z","timestamp":1717459200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Scientific Research Project of Department of Science &amp; Technology of Liaoning Province","award":["2023JH2\/20200171"],"award-info":[{"award-number":["2023JH2\/20200171"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Itampolin A, a natural brominated tyrosine alkaloid isolated from the sponge Iotrochota purpurea, has been shown to have good inhibitory effects in lung cancer cells as a p38\u03b1 inhibitor. A simple, sensitive, and reliable ultra-high-performance liquid chromatography\u2013tandem mass spectrometry (UHPLC-MS\/MS) method has been established, validated, and applied to the study of the pharmacokinetics and tissue distribution of itampolin A following intragastric and intravenous administration. Itampolin A and theophylline (internal standard, IS) were extracted by the simple protein precipitation technique using methanol as the precipitating solvent. Chromatographic separation was achieved by using the optimized mobile phase of a 0.1% formic acid aqueous solution and acetonitrile in the gradient elution mode. Itampolin A and IS were detected and quantified using positive electrospray ionization in the multiple reaction monitoring mode with transitions of m\/z 863.9 \u2192 569.1 for itampolin A and m\/z 181.1 \u2192 124.1 for IS, respectively. The assay exhibited a linear dynamic range of 1\u20131600 ng\/mL for itampolin A in biological samples and the low limit of quantification was 1 ng\/mL. Non-compartmental pharmacokinetic parameters indicated that itampolin A was well-absorbed into the systemic circulation and rapidly eliminated after administration. The apparent distribution volume of itampolin A was much higher after intragastric administration than that after intravenous administration. A tissue distribution study showed that itampolin A could be detected in different tissues and maintained a high concentration in the lung, which provided a material basis for its effective application in lung cancer. The pharmacokinetic process and tissue distribution characteristics of imtapolin A were expounded in this study, which can provide beneficial information for the further research and clinical application of itampolin A.<\/jats:p>","DOI":"10.3390\/molecules29112652","type":"journal-article","created":{"date-parts":[[2024,6,4]],"date-time":"2024-06-04T07:39:49Z","timestamp":1717486789000},"page":"2652","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Pharmacokinetics and Tissue Distribution of Itampolin A following Intragastric and Intravenous Administration in Rats Using Ultra-High-Performance Liquid Chromatography\u2013Tandem Mass Spectrometry"],"prefix":"10.3390","volume":"29","author":[{"given":"Qi","family":"Sun","sequence":"first","affiliation":[{"name":"School of Pharmacy, China Medical University, Shenyang 110122, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingwei","family":"Liang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Hainan Medical University, Haikou 570100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qingyu","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, China Medical University, Shenyang 110122, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuezhen","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, China Medical University, Shenyang 110122, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nan","family":"Zhao","sequence":"additional","affiliation":[{"name":"School of Pharmacy, China Medical University, Shenyang 110122, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1331-2164","authenticated-orcid":false,"given":"Fanhao","family":"Meng","sequence":"additional","affiliation":[{"name":"School of Pharmacy, China Medical University, Shenyang 110122, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,6,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Golfakhrabadi, F., Khaledi, M., Nazemi, M., and Safdarian, M. 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