{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T17:06:10Z","timestamp":1780679170768,"version":"3.54.1"},"reference-count":25,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2024,6,8]],"date-time":"2024-06-08T00:00:00Z","timestamp":1717804800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>An RP-HPLC method with a UV detector was developed for the simultaneous quantification of diclofenac diethylamine, methyl salicylate, and capsaicin in a pharmaceutical formulation and rabbit skin samples. The separation was achieved using a Thermo Scientific ACCLAIMTM 120 C18 column (Waltham, MA, USA, 4.6 mm \u00d7 150 mm, 5 \u00b5m). The optimized elution phase consisted of deionized water adjusted to pH = 3 using phosphoric acid mixed with acetonitrile in a 35:65% (v\/v) ratio with isocratic elution. The flow rate was set at 0.7 mL\/min, and the detection was performed at 205 nm and 25 \u00b0C. The method exhibits good linearity for capsaicin (0.05\u201370.0 \u00b5g\/mL), methyl salicylate (0.05\u2013100.0 \u00b5g\/mL), and diclofenac diethylamine (0.05\u2013100.0 \u00b5g\/mL), with low LOD values (0.0249, 0.0271, and 0.0038 for capsaicin, methyl salicylate, and diclofenac diethylamine, respectively). The RSD% values were below 3.0%, indicating good precision. The overall greenness score of the method was 0.61, reflecting its environmentally friendly nature. The developed RP-HPLC method was successfully applied to analyze Omni Hot Gel\u00ae pharmaceutical formulation and rabbit skin permeation samples.<\/jats:p>","DOI":"10.3390\/molecules29122732","type":"journal-article","created":{"date-parts":[[2024,6,10]],"date-time":"2024-06-10T08:59:06Z","timestamp":1718009946000},"page":"2732","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Development of an RP-HPLC Method for Quantifying Diclofenac Diethylamine, Methyl Salicylate, and Capsaicin in Pharmaceutical Formulation and Skin Samples"],"prefix":"10.3390","volume":"29","author":[{"given":"Lubna H. M.","family":"AlNahwa","sequence":"first","affiliation":[{"name":"Department of Chemistry, College of Science, Jouf University, P.O. Box 2014, Sakaka 72388, Aljouf, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0781-0850","authenticated-orcid":false,"given":"Hazim M.","family":"Ali","sequence":"additional","affiliation":[{"name":"Department of Chemistry, College of Science, Jouf University, P.O. Box 2014, Sakaka 72388, Aljouf, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamer H. A.","family":"Hasanin","sequence":"additional","affiliation":[{"name":"Department of Chemistry, College of Science, Jouf University, P.O. Box 2014, Sakaka 72388, Aljouf, Saudi Arabia"},{"name":"Department of Chemistry, Faculty of Science, Minia University, El-Minia 61519, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khaled","family":"Shalaby","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, College of Pharmacy, Jouf University, P.O. Box 2014, Sakaka 72388, Aljouf, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mutairah S.","family":"Alshammari","sequence":"additional","affiliation":[{"name":"Department of Chemistry, College of Science, Jouf University, P.O. Box 2014, Sakaka 72388, Aljouf, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alaa M.","family":"Alsirhani","sequence":"additional","affiliation":[{"name":"Department of Chemistry, College of Science, Jouf University, P.O. Box 2014, Sakaka 72388, Aljouf, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7549-3573","authenticated-orcid":false,"given":"Sabrein H.","family":"Mohamed","sequence":"additional","affiliation":[{"name":"Department of Chemistry, College of Science, Jouf University, P.O. Box 2014, Sakaka 72388, Aljouf, Saudi Arabia"},{"name":"Department of Chemistry, Faculty of Science, Cairo University, Giza 12613, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,6,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1360","DOI":"10.1001\/jama.2018.13103","article-title":"Diagnosis and Management of Rheumatoid Arthritis: A Review","volume":"320","author":"Aletaha","year":"2018","journal-title":"JAMA"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.vibspec.2015.12.005","article-title":"Quantitative Analysis of Topical Gels and Ointments by FT-Raman Spectroscopy","volume":"83","author":"Mazurek","year":"2016","journal-title":"Vib. Spectrosc."},{"key":"ref_3","unstructured":"(2007). 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