{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T05:17:42Z","timestamp":1775020662979,"version":"3.50.1"},"reference-count":45,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2024,6,12]],"date-time":"2024-06-12T00:00:00Z","timestamp":1718150400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Hue University","award":["NCM.DHH.2023.02"],"award-info":[{"award-number":["NCM.DHH.2023.02"]}]},{"name":"Core Research Program","award":["NCM.DHH.2023.02"],"award-info":[{"award-number":["NCM.DHH.2023.02"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>This study is the first to investigate the chemical composition and antioxidant, anti-inflammatory, and cytotoxic activities of Peperomia leptostachya leaf oil. A yellow oil was obtained through hydro-distillation, with a yield of 0.1% (w\/w). The GC-MS analysis revealed 66 compounds, constituting 99.6% of the oil. Sesquiterpene hydrocarbons predominated (70.4%), followed by monoterpene hydrocarbons (13.2%), oxygenated sesquiterpenes (12.4%), non-terpenic compounds (2.0%), and oxygenated monoterpenes (1.6%). Major constituents included germacrene D (25.1%), (E)-caryophyllene (17.4%), bicyclogermacrene (6.6%), \u03b1-pinene (6.2%), and \u03b2-pinene (4.7%). The assessment of antioxidant capacity via 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assay yielded a weak effect, with an IC50 value &gt; 100 \u00b5g\/mL. The inhibition of lipopolysaccharide-induced nitric oxide production in RAW 264.7 cells was quantified using the MTT assay, showing an IC50 value of 15.15 \u00b1 0.68 \u00b5g\/mL. Furthermore, cytotoxic effects on SK-LU-1 cell line growth were evaluated using the sulforhodamine B assay, resulting in an IC50 value of 37.45 \u00b1 2.43 \u03bcg\/mL. The anti-inflammatory activity was notable among the analyzed bioactivities of this oil. By employing a computational model, the predominant secondary metabolites in the essential oil were selected as candidates for interaction analysis with cyclooxygenase-2, an enzyme implicated in the inflammatory response. Our findings suggest that P. leptostachya leaf oil could serve as a potential source of natural compounds with prospective therapeutic effects in treating inflammatory conditions.<\/jats:p>","DOI":"10.3390\/molecules29122808","type":"journal-article","created":{"date-parts":[[2024,6,13]],"date-time":"2024-06-13T04:30:12Z","timestamp":1718253012000},"page":"2808","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Essential Oil from Vietnamese Peperomia leptostachya Hook. &amp; Arn. (Piperaceae): Chemical Composition, Antioxidant, Anti-Inflammatory, Cytotoxic Activities, and In Silico Analysis"],"prefix":"10.3390","volume":"29","author":[{"given":"Hien Minh","family":"Nguyen","sequence":"first","affiliation":[{"name":"Faculty of Pharmacy, Ton Duc Thang University, Ho Chi Minh City 700000, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6883-0823","authenticated-orcid":false,"given":"Ty Viet","family":"Pham","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry, University of Education, Hue University, 34 Le Loi, Hue 530000, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hung Quoc","family":"Vo","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Hue University of Medicine and Pharmacy, Hue University, 06 Ngo Quyen, Hue 530000, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hoai Thi","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Hue University of Medicine and Pharmacy, Hue University, 06 Ngo Quyen, Hue 530000, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linh Thuy Khanh","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Hue University of Medicine and Pharmacy, Hue University, 06 Ngo Quyen, Hue 530000, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9239-3774","authenticated-orcid":false,"given":"Bao Chi","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Department of Science, Technology & International Relations, Hue University, 04 Le Loi, Hue 530000, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khanh Linh","family":"Chung","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Ton Duc Thang University, Ho Chi Minh City 700000, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7442-3925","authenticated-orcid":false,"given":"Duc Viet","family":"Ho","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Hue University of Medicine and Pharmacy, Hue University, 06 Ngo Quyen, Hue 530000, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,6,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"de Sousa, D.P., Damasceno, R.O.S., Amorati, R., Elshabrawy, H.A., de Castro, R.D., Bezerra, D.P., Nunes, V.R.V., Gomes, R.C., and Lima, T.C. 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