{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T00:24:00Z","timestamp":1782433440462,"version":"3.54.5"},"reference-count":31,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2024,6,28]],"date-time":"2024-06-28T00:00:00Z","timestamp":1719532800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Consejo Nacional de Humanidades, Ciencia y Tecnolog\u00edas"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The Jatropha curcas cake, a protein-rich by-product of biofuel production, was the subject of our study. We identified and quantified the ACE inhibitory, antioxidant, and antidiabetic activities of bioactive peptides from a Jatropha curcas L. var Sevangel protein isolate. The protein isolate (20.44% recovered dry matter, 38.75% protein content, and 34.98% protein yield) was subjected to two enzyme systems for hydrolysis: alcalase (PEJA) and flavourzyme (PEJF), recording every 2 h until 8 h had passed. The highest proteolytic capacity in PEJA was reached at 2 h (4041.38 \u00b1 50.89), while in PEJF, it was reached at 6 h (3435.16 \u00b1 59.31). Gel electrophoresis of the PEJA and PEJF samples showed bands corresponding to peptides smaller than 10 kDa in both systems studied. The highest values for the antioxidant capacity (DPPH) were obtained at 4 h for PEJA (56.17 \u00b1 1.14), while they were obtained at 6 h for PEJF (26.64 \u00b1 0.52). The highest values for the antihypertensive capacity were recorded at 6 h (86.46 \u00b1 1.85) in PEJF. The highest antidiabetic capacity obtained for PEJA and PEJF was observed at 6 h, 68.86 \u00b1 8.27 and 52.75 \u00b1 2.23, respectively. This is the first report of their antidiabetic activity. Notably, alcalase hydrolysate outperformed flavourzyme hydrolysate and the cereals reported in other studies, confirming its better multi-bioactivity.<\/jats:p>","DOI":"10.3390\/molecules29133088","type":"journal-article","created":{"date-parts":[[2024,7,1]],"date-time":"2024-07-01T08:17:29Z","timestamp":1719821849000},"page":"3088","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["In Vitro Multi-Bioactive Potential of Enzymatic Hydrolysis of a Non-Toxic Jatropha curcas Cake Protein Isolate"],"prefix":"10.3390","volume":"29","author":[{"given":"Olloqui Enrique","family":"Javier","sequence":"first","affiliation":[{"name":"Centro de Biotecnolog\u00eda de Hongos Comestibles, Funcionales y Medicinales (CB-HCFM), Colegio de Postgraduados, Campus Puebla, Boulevard Forjadores de Puebla no. 205, Puebla 72760, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gonz\u00e1lez-Rodr\u00edguez Maurilio","family":"Alejandro","sequence":"additional","affiliation":[{"name":"\u00c1rea Acad\u00e9mica de Qu\u00edmica, Instituto de Ciencias B\u00e1sicas e Ingenier\u00eda, Universidad Aut\u00f3noma del Estado de Hidalgo, Carretera Pachuca-Tulancingo km 4.5, Mineral de la Reforma, Hidalgo 42185, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Contreras-L\u00f3pez","family":"Elizabeth","sequence":"additional","affiliation":[{"name":"\u00c1rea Acad\u00e9mica de Qu\u00edmica, Instituto de Ciencias B\u00e1sicas e Ingenier\u00eda, Universidad Aut\u00f3noma del Estado de Hidalgo, Carretera Pachuca-Tulancingo km 4.5, Mineral de la Reforma, Hidalgo 42185, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9654-3469","authenticated-orcid":false,"given":"P\u00e9rez-Flores Jes\u00fas","family":"Guadalupe","sequence":"additional","affiliation":[{"name":"\u00c1rea Acad\u00e9mica de Qu\u00edmica, Instituto de Ciencias B\u00e1sicas e Ingenier\u00eda, Universidad Aut\u00f3noma del Estado de Hidalgo, Carretera Pachuca-Tulancingo km 4.5, Mineral de la Reforma, Hidalgo 42185, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4268-9753","authenticated-orcid":false,"given":"P\u00e9rez-Escalante","family":"Emmanuel","sequence":"additional","affiliation":[{"name":"\u00c1rea Acad\u00e9mica de Qu\u00edmica, Instituto de Ciencias B\u00e1sicas e Ingenier\u00eda, Universidad Aut\u00f3noma del Estado de Hidalgo, Carretera Pachuca-Tulancingo km 4.5, Mineral de la Reforma, Hidalgo 42185, Mexico"},{"name":"Departamento de Biotecnolog\u00eda, Divisi\u00f3n de Ciencias Biol\u00f3gicas y de la Salud, Universidad Aut\u00f3noma Metropolitana, Campus Iztapalapa, Avenida San Rafael Atlixco 186, Mexico City 09340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moreno-Sece\u00f1a Juan","family":"Carlos","sequence":"additional","affiliation":[{"name":"Colegio de Postgraduados, Campus Montecillo, Carretera Mexico-Texcoco km 36.5, Texcoco 56264, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mart\u00ednez-Carrera","family":"Daniel","sequence":"additional","affiliation":[{"name":"Centro de Biotecnolog\u00eda de Hongos Comestibles, Funcionales y Medicinales (CB-HCFM), Colegio de Postgraduados, Campus Puebla, Boulevard Forjadores de Puebla no. 205, Puebla 72760, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,6,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Mitra, S., Ghose, A., Gujre, N., Senthilkumar, S., Borah, P., Paul, A., and Rangan, L. 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