{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:48:13Z","timestamp":1790729293134,"version":"4.1.0"},"reference-count":89,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2024,6,28]],"date-time":"2024-06-28T00:00:00Z","timestamp":1719532800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Department of Physics and Biophysics of Wroclaw University of Environmental and Life Sciences","award":["B010\/0014\/24"],"award-info":[{"award-number":["B010\/0014\/24"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Sea buckthorn and Japanese knotweed are known in many traditional medicine systems to be a great source of bioactive substances. This research aims to compare the bioactivity and protective effects of the phenolic extracts of leaves from sea buckthorn and roots and leaves from the Japanese knotweed on erythrocytes. The polyphenol composition of the extract was analyzed using UPLC-PDA-ESI-MS\/MS. The extracts\u2019 toxicity and impact on the erythrocytes\u2019 osmotic fragility were measured spectrophotometrically. The antioxidant activity was determined based on the inhibition of oxidation of erythrocytes and their membrane induced by 2,2\u2032-Azobis(2-methylpropionamidine) dihydrochloride (AAPH),measured spectrophotometrically and using fluorimetry. To find the possible mechanism of the extracts\u2019 action, extract-modified cells were observed under a microscope, and the potential localization of the extract\u2019s phytochemical composition was checked using fluorescent probes. The results showed that the used extracts are not toxic to erythrocytes, increase their osmotic resistance, and successfully protect them against free radicals. Extract components localize on the outer part of the membrane, where they can scavenge the free radicals from the environment. Altogether, the presented extracts can greatly protect living organisms against free radicals and can be used to support the treatment of diseases caused by excess free radicals.<\/jats:p>","DOI":"10.3390\/molecules29133090","type":"journal-article","created":{"date-parts":[[2024,7,1]],"date-time":"2024-07-01T08:17:29Z","timestamp":1719821849000},"page":"3090","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Protective Effect of Polyphenolic Extracts from Hippophae rhamnoides L. and Reynoutria japonica Houtt. on Erythrocyte Membrane"],"prefix":"10.3390","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-9489-5732","authenticated-orcid":false,"given":"Teresa","family":"Ka\u017amierczak","sequence":"first","affiliation":[{"name":"Department of Physics and Biophysics, The Faculty of Biotechnology and Food Sciences, Wroc\u0142aw University of Environmental and Life Sciences, Norwida Str. 25, 50-375 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8650-9171","authenticated-orcid":false,"given":"Katarzyna","family":"M\u0119czarska","sequence":"additional","affiliation":[{"name":"Department of Physics and Biophysics, The Faculty of Biotechnology and Food Sciences, Wroc\u0142aw University of Environmental and Life Sciences, Norwida Str. 25, 50-375 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6182-0211","authenticated-orcid":false,"given":"Sabina","family":"Lachowicz-Wi\u015bniewska","sequence":"additional","affiliation":[{"name":"Faculty of Medicine and Health Sciences, Calisia University, 62-800 Kalisz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8191-0804","authenticated-orcid":false,"given":"Sylwia","family":"Cyboran-Miko\u0142ajczyk","sequence":"additional","affiliation":[{"name":"Department of Physics and Biophysics, The Faculty of Biotechnology and Food Sciences, Wroc\u0142aw University of Environmental and Life Sciences, Norwida Str. 25, 50-375 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Oszmia\u0144ski","sequence":"additional","affiliation":[{"name":"Departament of Fruit, Vegetable and Plant Nutraceutical Technology, The Faculty of Biotechnology and Food Sciences, Wroc\u0142aw University of Environmental and Life Sciences, Che\u0142mo\u0144skiego Str. 37, 51-630 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorota","family":"Bonarska-Kujawa","sequence":"additional","affiliation":[{"name":"Department of Physics and Biophysics, The Faculty of Biotechnology and Food Sciences, Wroc\u0142aw University of Environmental and Life Sciences, Norwida Str. 25, 50-375 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,6,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2982","DOI":"10.1016\/j.jacc.2020.11.010","article-title":"Global Burden of Cardiovascular Diseases and Risk Factors, 1990\u20132019","volume":"76","author":"Roth","year":"2020","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Rana, A., Samtiya, M., Dhewa, T., Mishra, V., and Aluko, R.E. (2022). Health Benefits of Polyphenols: A Concise Review. J. Food Biochem., 46.","DOI":"10.1111\/jfbc.14264"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Poga\u010dnik, L., Bergant, T., Skrt, M., Ulrih, N.P., Viktorov\u00e1, J., and Ruml, T. (2020). In Vitro Comparison of the Bioactivities of Japanese and Bohemian Knotweed Ethanol Extracts. Foods, 9.","DOI":"10.3390\/foods9050544"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"17","DOI":"10.31482\/mmsl.2017.004","article-title":"Biologically Active Compounds of Knotweed (Reynoutria spp.)","volume":"86","author":"Ovando","year":"2017","journal-title":"Mil. Med. Sci. Lett."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"687","DOI":"10.3923\/jbs.2004.687.693","article-title":"Important Therapeutic Uses of Sea Buckthorn (Hippophae): A Review","volume":"4","author":"Zeb","year":"2004","journal-title":"J. Biol. Sci."},{"key":"ref_6","first-page":"177","article-title":"The Genus Hippopha\u00eb L. A Taxonomic Study","volume":"8","author":"Rousi","year":"1971","journal-title":"Ann. Bot. Fenn."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/S0924-2244(02)00136-X","article-title":"Composition and Physiological Effects of Sea Buckthorn (Hippopha\u00eb) lipids","volume":"13","author":"Yang","year":"2002","journal-title":"Trends Food Sci. Technol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1939","DOI":"10.1021\/jf001059s","article-title":"Fatty Acid Composition of Lipids in Sea Buckthorn (Hippopha\u00eb rhamnoides L.) Berries of Different Origins","volume":"49","author":"Yang","year":"2001","journal-title":"J. Agric. Food Chem."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Criste, A., Urcan, A.C., Bunea, A., Furtuna, F.R.P., Olah, N.K., Madden, R.H., and Corcionivoschi, N. (2020). Phytochemical Composition and Biological Activity of Berries and Leaves from Four Romanian Sea Buckthorn (Hippophae rhamnoides L.) Varieties. Molecules, 25.","DOI":"10.3390\/molecules25051170"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"E514","DOI":"10.1111\/j.1365-2621.2005.tb08312.x","article-title":"Effect of Drying on the Nutraceutical Quality of Sea Buckthorn (Hippophae rhamnoides L. ssp. sinensis) Leaves","volume":"70","author":"Guan","year":"2006","journal-title":"J. Food Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1016\/S0955-2863(02)00179-1","article-title":"Effects of an Antioxidant-Rich Juice (Sea buckthorn) on Risk Factors for Coronary Heart Disease in Humans","volume":"13","author":"Eccleston","year":"2002","journal-title":"J. Nutr. Biochem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1002\/ptr.831","article-title":"Antiulcerogenic Effect of Hippophae rhamnoides L.","volume":"15","author":"Demirezer","year":"2001","journal-title":"Phytotherapy Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"659705","DOI":"10.1093\/ecam\/nep189","article-title":"Mechanism of Wound-Healing Activity of Hippophae rhamnoides L. Leaf Extract in Experimental Burns","volume":"2011","author":"Upadhyay","year":"2011","journal-title":"Evid. Based Complement. Altern. Med."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.1007\/s10530-008-9381-4","article-title":"Asexual Spread versus Sexual Reproduction and Evolution in Japanese Knotweed s.l. Sets the Stage for the \u201cBattle of the Clones\u201d","volume":"11","author":"Bailey","year":"2009","journal-title":"Biol. Invasions"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1007\/s11655-012-1060-8","article-title":"The Progress of the Research on Cardio-Vascular Effects and Acting Mechanism of Polydatin","volume":"18","author":"Liu","year":"2012","journal-title":"Chin. J. Integr. Med."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1652","DOI":"10.3892\/ijmm.2016.2554","article-title":"Polydatin Inhibits the Oxidative Stress-Induced Proliferation of Vascular Smooth Muscle Cells by Activating the eNOS\/SIRT1 Pathway","volume":"37","author":"Ma","year":"2016","journal-title":"Int. J. Mol. Med."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1186\/s12935-021-02280-5","article-title":"Resveratrol Mediates Its Anti-Cancer Effects by Nrf2 Signaling Pathway Activation","volume":"21","author":"Alavi","year":"2021","journal-title":"Cancer Cell Int."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Ko, J.-H., Sethi, G., Um, J.-Y., Shanmugam, M.K., Arfuso, F., Kumar, A.P., Bishayee, A., and Ahn, K.S. (2017). The Role of Resveratrol in Cancer Therapy. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18122589"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Leung, S.W., Lai, J.H., Wu, J.C.-C., Tsai, Y.-R., Chen, Y.-H., Kang, S.-J., Chiang, Y.-H., Chang, C.-F., and Chen, K.-Y. (2020). Neuroprotective Effects of Emodin against Ischemia\/Reperfusion Injury through Activating ERK-1\/2 Signaling Pathway. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21082899"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Wr\u00f3bel-Biedrawa, D., Grabowska, K., Galanty, A., Sobolewska, D., and Podolak, I. (2022). A Flavonoid on the Brain: Quercetin as a Potential Therapeutic Agent in Central Nervous System Disorders. Life, 12.","DOI":"10.3390\/life12040591"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Sharifi-Rad, M., Anil Kumar, N.V., Zucca, P., Varoni, E.M., Dini, L., Panzarini, E., Rajkovic, J., Tsouh Fokou, P.V., Azzini, E., and Peluso, I. (2020). Lifestyle, Oxidative Stress, and Antioxidants: Back and Forth in the Pathophysiology of Chronic Diseases. Front. Physiol., 11.","DOI":"10.3389\/fphys.2020.00694"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Ka\u017amierczak, T., Bonarska-Kujawa, D., M\u0119czarska, K., Cyboran-Miko\u0142ajczyk, S., Oszmia\u0144ski, J., and Kapusta, I. (2023). Analysis of the Polyphenolic Composition of Vaccinium L. Extracts and Their Protective Effect on Red Blood Cell Membranes. Membranes, 13.","DOI":"10.3390\/membranes13060589"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1962","DOI":"10.1002\/rra.3725","article-title":"Invasive Japanese Knotweed (Reynoutria japonica Houtt.) and Related Knotweeds as Catalysts for Streambank Erosion","volume":"36","author":"Colleran","year":"2020","journal-title":"River Res. Appl."},{"key":"ref_24","unstructured":"Carter, B., Curtis, T.G.F., and Sheehy-Skeffington, M.J. (1992, January 17\u201321). Coastal Dunes: Geomorphology, Ecology and Management for Conservation. Proceedings of the Third European Dune Congress, Galway, Ireland."},{"key":"ref_25","unstructured":"Tsuzuku, S. (2013). Local Knowledge about Japanese Vegetables and Herbs among People of Japanese Descent in Southwest British Columbia. [Ph.D. Thesis, University of British Columbia]."},{"key":"ref_26","first-page":"483","article-title":"Allium ursinum L. leaves components modified the physico-chemical properties of red blood cells protecting them from the effects of oxidative stress","volume":"76","year":"2019","journal-title":"Acta Pol. Pharm."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1007\/s00232-013-9566-3","article-title":"Activity of Hawthorn Leaf and Bark Extracts in Relation to Biological Membrane","volume":"246","author":"Kapusta","year":"2013","journal-title":"J. Membr. Biol."},{"key":"ref_28","first-page":"721","article-title":"Red Cell Shapes. An Illustrated Classification and Its Rationale","volume":"12","author":"Bessis","year":"1972","journal-title":"Nouv. Rev. Fr. Hematol."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Soares, S., Brand\u00e3o, E., Guerreiro, C., Soares, S., Mateus, N., and de Freitas, V. (2020). Tannins in Food: Insights into the Molecular Perception of Astringency and Bitter Taste. Molecules, 25.","DOI":"10.3390\/molecules25112590"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1516","DOI":"10.2527\/1995.7351516x","article-title":"Nutritional Toxicology of Tannins and Related Polyphenols in Forage Legumes","volume":"73","author":"Reed","year":"1995","journal-title":"J. Anim. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1002\/jat.2550100504","article-title":"Prevention of Hydrolysable Tannin Toxicity in Goats Fed Clidemia hirta by Calcium Hydroxide Supplementation","volume":"10","author":"Murdiati","year":"1990","journal-title":"J. Appl. Toxicol."},{"key":"ref_32","first-page":"228","article-title":"Chemical Properties and Immunobiological Activities of Streptococcal Lipoteichoic Acids","volume":"259","author":"Hamada","year":"1985","journal-title":"Zentralblatt F\u00fcr Bakteriol. Mikrobiol. Und Hygiene. Ser. A Med. Microbiol. Infect. Dis. Virol. Parasitol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/S0924-8579(02)00349-7","article-title":"In Vitro Activity of Brazilian Medicinal Plants, Naturally Occurring Naphthoquinones and Their Analogues, against Methicillin-Resistant Staphylococcus aureus","volume":"21","author":"Machado","year":"2003","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1111\/j.1348-0421.2004.tb03521.x","article-title":"Antibacterial Activity of Hydrolyzable Tannins Derived from Medicinal Plants against Helicobacter pylori","volume":"48","author":"Funatogawa","year":"2004","journal-title":"Microbiol. Immunol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1179","DOI":"10.2174\/138955708786140990","article-title":"Antimicrobial and Antiviral Activity of Hydrolysable Tannins","volume":"8","author":"Buzzini","year":"2008","journal-title":"Mini-Rev. Med. Chem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1887","DOI":"10.1021\/jf970975b","article-title":"High Molecular Weight Plant Polyphenolics (Tannins) as Biological Antioxidants","volume":"46","author":"Hagerman","year":"1998","journal-title":"J. Agric. Food Chem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"10","DOI":"10.4236\/ojmip.2016.61002","article-title":"Protective Effects of Polyphenol Extracts from Sea Buckthorn (Hippopha\u00eb rhamnoides L.) on Rat Hearts","volume":"06","author":"Tang","year":"2016","journal-title":"Open J. Mol. Integr. Physiol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"12044","DOI":"10.1038\/s41598-019-48610-6","article-title":"A Semi-Continuous Process for Polyphenols Extraction From Sea Buckthorn Leaves","volume":"9","author":"Asofiei","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1080\/00986445.2015.1134518","article-title":"Microwave-Assisted Batch Extraction of Polyphenols from Sea Buckthorn Leaves","volume":"203","author":"Asofiei","year":"2016","journal-title":"Chem. Eng. Commun."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"7678","DOI":"10.1021\/jf061018h","article-title":"Effects of Different Organic Farming Methods on the Concentration of Phenolic Compounds in Sea Buckthorn Leaves","volume":"54","author":"Pusenius","year":"2006","journal-title":"J. Agric. Food Chem."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.3390\/nu2121231","article-title":"Chemistry and Biochemistry of Dietary Polyphenols","volume":"2","author":"Tsao","year":"2010","journal-title":"Nutrients"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1593","DOI":"10.1016\/S0006-2952(00)00488-3","article-title":"The Uncoupling Efficiency and Affinity of Flavonoids for Vesicles","volume":"60","author":"Driessen","year":"2000","journal-title":"Biochem. Pharmacol."},{"key":"ref_43","first-page":"135","article-title":"Active Oxygen Free Radicals are Scavenged by Condensed Tannins","volume":"280","author":"Uchida","year":"1988","journal-title":"Prog. Clin. Biol. Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1080\/15216549800202982","article-title":"Esr Studies of Vitamin c Regeneration, Order of Reactivity of Natural Source Phytochemical Preparations","volume":"45","author":"Cossins","year":"1998","journal-title":"IUBMB Life"},{"key":"ref_45","first-page":"380","article-title":"Antiinflammatory Activities of Procyanidin-Containing Extracts from Pinus Pinaster Ait. after Oral and Cutaneous Application","volume":"52","author":"Rohdewald","year":"1997","journal-title":"Pharmazie"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1055\/s-2006-957956","article-title":"Procyanidines from Vitis vinifera Seeds Protect Rabbit Heart from Ischemia\/Reperfusion Injury: Antioxidant Intervention and\/or Iron and Copper Sequestering Ability","volume":"62","author":"Carini","year":"1996","journal-title":"Planta Medica"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"704","DOI":"10.1016\/S0891-5849(99)00090-8","article-title":"Antioxidant Activity and Biologic Properties of a Procyanidin-Rich Extract from Pine (Pinus maritima) Bark, Pycnogenol","volume":"27","author":"Packer","year":"1999","journal-title":"Free. Radic. Biol. Med."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Jug, U., Naumoska, K., and Vovk, I. (2021). (\u2212)-Epicatechin\u2014An Important Contributor to the Antioxidant Activity of Japanese Knotweed Rhizome Bark Extract as Determined by Antioxidant Activity-Guided Fractionation. Antioxidants, 10.","DOI":"10.3390\/antiox10010133"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"8131","DOI":"10.1039\/C6NJ01294A","article-title":"Glucose Oxidase-Assisted Extraction of Resveratrol from Japanese Knotweed (Fallopia japonica)","volume":"40","author":"Chen","year":"2016","journal-title":"New J. Chem."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Bensa, M., Glavnik, V., and Vovk, I. (2020). Leaves of Invasive Plants\u2014Japanese, Bohemian and Giant Knotweed\u2014The Promising New Source of Flavan-3-ols and Proanthocyanidins. Plants, 9.","DOI":"10.3390\/plants9010118"},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Apak, R., Capanoglu, E., and Shahidi, F. (2017). Folin-Ciocalteu Method for the Measurement of Total Phenolic Content and Antioxidant Capacity. Measurement of Antioxidant Activity & Capacity, John Wiley and Sons, Ltd.","DOI":"10.1002\/9781119135388"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"17543","DOI":"10.1021\/acs.jafc.3c04022","article-title":"The Chemistry Behind the Folin\u2013Ciocalteu Method for the Estimation of (Poly)Phenol Content in Food: Total Phenolic Intake in a Mediterranean Dietary Pattern","volume":"71","year":"2023","journal-title":"J. Agric. Food Chem."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.lfs.2014.12.025","article-title":"Concentrated Green Tea Supplement: Biological Activity and Molecular Mechanisms","volume":"126","author":"Cyboran","year":"2015","journal-title":"Life Sci."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1007\/978-1-4684-5730-8_25","article-title":"Radical Chemistry of Flavonoid Antioxidants","volume":"264","author":"Bors","year":"1990","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Platzer, M., Kiese, S., Herfellner, T., Schweiggert-Weisz, U., and Eisner, P. (2021). How Does the Phenol Structure Influence the Results of the Folin-Ciocalteu Assay?. Antioxidants, 10.","DOI":"10.3390\/antiox10050811"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1841","DOI":"10.1021\/jf030723c","article-title":"The Chemistry behind Antioxidant Capacity Assays","volume":"53","author":"Huang","year":"2005","journal-title":"J. Agric. Food Chem."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"8139","DOI":"10.1021\/jf1005935","article-title":"Thorough Study of Reactivity of Various Compound Classes toward the Folin-Ciocalteu Reagent","volume":"58","author":"Everette","year":"2010","journal-title":"J. Agric. Food Chem."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"5990","DOI":"10.1039\/c3ay41125g","article-title":"The Folin\u2013Ciocalteu Assay Revisited: Improvement of Its Specificity for Total Phenolic Content Determination","volume":"5","author":"Benavides","year":"2013","journal-title":"Anal. Methods"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"19180","DOI":"10.3390\/molecules191119180","article-title":"Antioxidant Property of Coffee Components: Assessment of Methods that Define Mechanisms of Action","volume":"19","author":"Liang","year":"2014","journal-title":"Molecules"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2019\/9703176","article-title":"Total Phenolic, Anthocyanin, Catechins, DPPH Radical Scavenging Activity, and Toxicity of Lepisanthes alata (Blume) Leenh","volume":"2019","author":"Anggraini","year":"2019","journal-title":"Int. J. Food Sci."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1016\/0891-5849(96)00237-7","article-title":"Scavenging Effects of Tea Catechins and Their Derivatives on 1,1-Diphenyl-2-Picrylhydrazyl Radical","volume":"21","author":"Nanjo","year":"1996","journal-title":"Free. Radic. Biol. Med."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"124","DOI":"10.4103\/0974-8520.115451","article-title":"In Vitro Evaluation of Antioxidant Activity of Cordia Dichotoma (Forst f.) Bark","volume":"34","author":"Nariya","year":"2013","journal-title":"Ayu"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1007\/s10787-017-0345-0","article-title":"Anti-Inflammatory Activity of the Functional Groups Present in Hippophae rhamnoides (Seabuckthorn) Leaf Extract","volume":"26","author":"Tanwar","year":"2017","journal-title":"Inflammopharmacology"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"3443","DOI":"10.1016\/j.fct.2010.09.019","article-title":"Antioxidant, Cytoprotective and Antibacterial Effects of Sea buckthorn (Hippophae rhamnoides L.) Leaves","volume":"48","author":"Upadhyay","year":"2010","journal-title":"Food Chem. Toxicol."},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Kov\u00e1\u0159ov\u00e1, M., Mad\u011bra, P., Frant\u00edk, T., Nov\u00e1k, J., and Vencl, \u0160. (2022). Effects of Knotweed-Enriched Feed on the Blood Characteristics and Fitness of Horses. Agriculture, 12.","DOI":"10.3390\/agriculture12010109"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1016\/j.indcrop.2014.02.021","article-title":"Phytochemical Constituents, Antioxidant Activity, Cytotoxicity and Osmotic Fragility Effects of Caju (Anacardium microcarpum)","volume":"55","author":"Filho","year":"2014","journal-title":"Ind. Crop. Prod."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1006\/abbi.1994.1053","article-title":"A Mechanism for the Stimulatory Effect of Aluminum on Iron-Induced Lipid Peroxidation","volume":"308","author":"Oteiza","year":"1994","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/S0009-8981(02)00300-5","article-title":"Measurement of Erythrocyte Lipids, Lipid Peroxidation, Antioxidants and Osmotic Fragility in Cervical Cancer Patients","volume":"326","author":"Kolanjiappan","year":"2002","journal-title":"Clin. Chim. Acta"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"333","DOI":"10.2478\/s11658-012-0014-7","article-title":"Stabilization of Erythrocytes Against Oxidative and Hypotonic Stress by Tannins Isolated from Sumac Leaves (Rhus typhina L.) and Grape Seeds (Vitis vinifera L.)","volume":"17","author":"Olchowik","year":"2012","journal-title":"Cell. Mol. Biol. Lett."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1515\/pjfns-2017-0021","article-title":"In Vitro Studies of Anti-Hemolytic and Cytotoxic Activity of Procyanidin-Rich Extract from the Leaves of Actinidia Arguta","volume":"68","author":"Csonka","year":"2018","journal-title":"Pol. J. Food Nutr. Sci."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1007\/s11746-003-0812-z","article-title":"Antioxidant Activity of Phenolic Compounds in 2,2\u2032-Azobis (2-Amidinopropane) Dihydrochloride (AAPH)-Induced Oxidation: Synergistic and Antagonistic Effects","volume":"80","author":"Cuvelier","year":"2003","journal-title":"J. Am. Oil Chem. Soc."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"3934","DOI":"10.1021\/jf8034868","article-title":"In Vivo, in Vitro, and in Silico Studies of Cu\/Zn-Superoxide Dismutase Regulation by Molecules in Grape Seed Procyanidin Extract","volume":"57","author":"Sala","year":"2009","journal-title":"J. Agric. Food Chem."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1007\/s002490050140","article-title":"Amphiphile Induced Echinocyte-Spheroechinocyte Transformation of Red Blood Cell Shape","volume":"27","year":"1998","journal-title":"Eur. Biophys. J."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/BFb0027721","article-title":"Properties and Structural Basis of Simple Diffusion Pathways in the Erythrocyte Membrane","volume":"78","author":"Deuticke","year":"1977","journal-title":"Rev. Physiol. Biochem. Pharmacol."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"1220","DOI":"10.1016\/S0006-3495(97)78769-1","article-title":"Membrane Potential and Human Erythrocyte Shape","volume":"72","author":"Gedde","year":"1997","journal-title":"Biophys. J."},{"key":"ref_76","doi-asserted-by":"crossref","unstructured":"Virtanen, V., and Karonen, M. (2020). Partition Coefficients (logP) of Hydrolysable Tannins. Molecules, 25.","DOI":"10.3390\/molecules25163691"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s11101-017-9507-3","article-title":"Procyanidins: A Comprehensive Review Encompassing Structure Elucidation via Mass Spectrometry","volume":"17","author":"Rue","year":"2018","journal-title":"Phytochem. Rev."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1007\/978-1-0716-2954-3_21","article-title":"DPH Probe Method for Liposome-Membrane Fluidity Determination","volume":"2622","author":"He","year":"2023","journal-title":"Methods Mol. Biol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"46","DOI":"10.3109\/09687688.2015.1031833","article-title":"Molecular Mechanism of Action of Chlorogenic Acid on Erythrocyte and Lipid Membranes","volume":"32","year":"2015","journal-title":"Mol. Membr. Biol."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"1362","DOI":"10.1016\/j.bbamem.2017.04.026","article-title":"Interaction of Procyanidin B3 with Membrane Lipids\u2014Fluorescence, DSC and FTIR Studies","volume":"1859","author":"Jurkiewicz","year":"2017","journal-title":"Biochim. Biophys. Acta (BBA)\u2014Biomembr."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/S0304-3835(97)00248-6","article-title":"Antimutagenic Activity of Anthocyanins Isolated from Aronia Melanocarpa Fruits","volume":"119","author":"Gasiorowski","year":"1997","journal-title":"Cancer Lett."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"6852","DOI":"10.3390\/molecules18066852","article-title":"Application and Analysis of the Folin Ciocalteu Method for the Determination of the Total Phenolic Content from Limonium brasiliense L.","volume":"18","author":"Blainski","year":"2013","journal-title":"Molecules"},{"key":"ref_83","doi-asserted-by":"crossref","unstructured":"Ger\u00e7ek, E., Zengin, H., Eri\u015fir, F.E., and Y\u0131lmaz, \u00d6. (2021). Biochemical Changes and Antioxidant Capacity of Naringin and Naringenin against Malathion Toxicity in Saccharomyces Cerevisiae. Comp. Biochem. Physiol. Part C Toxicol. Pharmacol., 241.","DOI":"10.1016\/j.cbpc.2020.108969"},{"key":"ref_84","doi-asserted-by":"crossref","unstructured":"Cyboran-Miko\u0142ajczyk, S., M\u0119czarska, K., Solarska-\u015aciuk, K., Ratajczak-Wielgomas, K., Oszmia\u0144ski, J., Jencova, V., and Bonarska-Kujawa, D. (2022). Protection of Erythrocytes and Microvascular Endothelial Cells against Oxidative Damage by Fragaria vesca L. and Rubus idaeus L. Leaves Extracts\u2014The Mechanism of Action. Molecules, 27.","DOI":"10.3390\/molecules27185865"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/0003-9861(63)90042-0","article-title":"The Preparation and Chemical Characteristics of Hemoglobin-Free Ghosts of Human Erythrocytes","volume":"100","author":"Dodge","year":"1963","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"ref_87","first-page":"660","article-title":"Polyphenol Content and Bioactivity of Saskatoon (Amelanchier alnifolia nutt.) Leaves and Berries","volume":"74","author":"Kleszczynska","year":"2017","journal-title":"Acta Pol. Pharm."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1007\/BF02796139","article-title":"TMA-DPH: A Suitable Fluorescence Polarization Probe for Specific Plasma Membrane Fluidity Studies in Intact Living Cells","volume":"5","author":"Kuhry","year":"1983","journal-title":"Cell Biophys."},{"key":"ref_89","first-page":"4","article-title":"PAST: Paleontological statistics software package for education and data analysis","volume":"4","author":"Hammer","year":"2001","journal-title":"Palaeontol. Electron."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/29\/13\/3090\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:07:20Z","timestamp":1760108840000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/29\/13\/3090"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6,28]]},"references-count":89,"journal-issue":{"issue":"13","published-online":{"date-parts":[[2024,7]]}},"alternative-id":["molecules29133090"],"URL":"https:\/\/doi.org\/10.3390\/molecules29133090","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6,28]]}}}