{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T22:33:27Z","timestamp":1792794807684,"version":"4.5.0"},"reference-count":53,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2024,7,20]],"date-time":"2024-07-20T00:00:00Z","timestamp":1721433600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Key Laboratory for Quality Control of Traditional Chinese Medicine (Tibetan Medicine) of the National Medical Products Administration","award":["XZSYJY-YJKYXM-2023-02"],"award-info":[{"award-number":["XZSYJY-YJKYXM-2023-02"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Polysaccharide is one of the principal bioactive components found in medicinal mushrooms and has been proven to enhance host immunity. However, the possible mechanism of immunomodulatory activity of Cordyceps militaris polysaccharide is not fully understood. Hot water extraction and alcohol precipitation, DEAE-Sephadex A-25 chromatography, and Sephadex G-100 chromatography were used to isolate polysaccharide from C. militaris. A high-molecular-weight polysaccharide isolated from C. militaris was designated as HCMP, which had an Mw of 6.18 \u00d7 105 Da and was composed of arabinose, galactose, glucose, mannose, and xylose in a mole ratio of 2.00:8.01:72.54:15.98:1.02. The polysaccharide content of HCMP was 91.2% \u00b1 0.16. The test in vitro showed that HCMP activated mouse macrophage RAW 264.7 cells by enhancing phagocytosis and NO production, and by regulating mRNA expressions of inflammation-related molecules in RAW 264.7 cells. Western blotting revealed that HCMP induced the phosphorylation of mitogen-activated protein kinases (MAPKs). Moreover, using inhibitors of MAPKs decreased the mRNA levels of inflammation-related molecules induced by HCMP. These data evidenced that the immunomodulatory effect of HCMP on RAW 264.7 macrophages was mediated via the MAPK signaling pathway. These findings suggested that HCMP could be developed as a potent immunomodulatory agent for use in functional foods and dietary supplements.<\/jats:p>","DOI":"10.3390\/molecules29143408","type":"journal-article","created":{"date-parts":[[2024,7,22]],"date-time":"2024-07-22T14:45:53Z","timestamp":1721659553000},"page":"3408","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Immunomodulatory Effect of Cordyceps militaris Polysaccharide on RAW 264.7 Macrophages by Regulating MAPK Signaling Pathways"],"prefix":"10.3390","volume":"29","author":[{"given":"Yan","family":"Liu","sequence":"first","affiliation":[{"name":"School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China"},{"name":"Engineering Research Center of Therapeutic Antibody (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200240, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayi","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China"},{"name":"Engineering Research Center of Therapeutic Antibody (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200240, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhijian","family":"Guo","sequence":"additional","affiliation":[{"name":"School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China"},{"name":"Engineering Research Center of Therapeutic Antibody (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200240, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7737-7787","authenticated-orcid":false,"given":"Qizhang","family":"Li","sequence":"additional","affiliation":[{"name":"Innovative Drug R&D Center, College of Life Sciences, Huaibei Normal University, Huaibei 235000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lida","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China"},{"name":"Engineering Research Center of Therapeutic Antibody (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200240, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingxia","family":"Zhao","sequence":"additional","affiliation":[{"name":"School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China"},{"name":"Engineering Research Center of Therapeutic Antibody (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200240, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3993-6346","authenticated-orcid":false,"given":"Xuanwei","family":"Zhou","sequence":"additional","affiliation":[{"name":"School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China"},{"name":"Engineering Research Center of Therapeutic Antibody (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200240, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Gong, W., Han, R., Li, H., Song, J., Yan, H., Li, G., Liu, A., Cao, X., Guo, J., and Zhai, S. (2017). Agronomic traits and molecular marker identification of wheat-Aegilops caudata addition lines. Front. Plant Sci., 8.","DOI":"10.3389\/fpls.2017.01743"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1016\/j.ijbiomac.2019.08.174","article-title":"A polysaccharide isolated from the fruits of Physalis alkekengi L. induces RAW264. 7 macrophages activation via TLR2 and TLR4-mediated MAPK and NF-\u03baB signaling pathways","volume":"140","author":"Yang","year":"2019","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1152","DOI":"10.1007\/s00253-006-0411-9","article-title":"Hypoglycemic activity of the fungi Cordyceps militaris, Cordyceps sinensis, Tricholoma mongolicum, and Omphalia lapidescens in streptozotocin-induced diabetic rats","volume":"72","author":"Zhang","year":"2006","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1016\/j.nutres.2015.04.011","article-title":"Cordycepin from Cordyceps militaris prevents hyperglycemia in alloxan-induced diabetic mice","volume":"35","author":"Ma","year":"2015","journal-title":"Nutr. Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1615\/IntJMedMushr.v15.i3.30","article-title":"Antioxidant and immunomodulating activities of exo- and endopolysaccharide fractions from submerged mycelia cultures of culinary-medicinal mushrooms","volume":"15","author":"Jeong","year":"2013","journal-title":"Int. J. Med. Mushrooms"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"93712","DOI":"10.18632\/oncotarget.21477","article-title":"Cordycepin and a preparation from Cordyceps militaris inhibit malignant transformation and proliferation by decreasing EGFR and IL-17RA signaling in a murine oral cancer model","volume":"8","author":"Hsu","year":"2017","journal-title":"Oncotarget"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Wang, Y., Zhang, Q., Chen, Y., Liang, C.L., Liu, H., Qiu, F., and Dai, Z. (2020). Antitumor effects of immunity-enhancing traditional Chinese medicine. Biomed. Pharmacother., 121.","DOI":"10.1016\/j.biopha.2019.109570"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1016\/j.ijbiomac.2017.07.104","article-title":"Immunomodulatory effects of hydroxyethylated Hericium erinaceus polysaccharide on macrophages RAW264.7","volume":"105","author":"Ren","year":"2017","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.ijbiomac.2018.03.062","article-title":"Purification, partial characterization and inducing tumor cell apoptosis activity of a polysaccharide from Ganoderma applanatum","volume":"115","author":"Zhen","year":"2018","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"127798","DOI":"10.1016\/j.foodchem.2020.127798","article-title":"Production, physicochemical characteristics, and in vitro biological activities of polysaccharides obtained from fresh bitter gourd (Momordica charantia L.) via room temperature extraction techniques","volume":"337","author":"Yan","year":"2021","journal-title":"Food Chem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"115969","DOI":"10.1016\/j.carbpol.2020.115969","article-title":"Structural characterization, antioxidant and immunomodulatory activities of a neutral polysaccharide from Cordyceps militaris cultivated on hull-less barley","volume":"235","author":"Zhang","year":"2020","journal-title":"Carbohydr. Polym."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1211\/jpp.61.03.0002","article-title":"Cordyceps fungi: Natural products, pharmacological functions and developmental products","volume":"61","author":"Zhou","year":"2009","journal-title":"J. Pharm. Pharmacol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.ijbiomac.2019.12.115","article-title":"Structural characterization and immune-enhancing activity of a novel high-molecular-weight polysaccharide from Cordyceps militaris","volume":"145","author":"He","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1316","DOI":"10.1039\/D1FO03630K","article-title":"Sulfated Chinese yam polysaccharide enhances the immunomodulatory activity of RAW 264.7 cells via the TLR4-MAPK\/NF-\u03baB signaling pathway","volume":"13","author":"Liu","year":"2022","journal-title":"Food Funct."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"96","DOI":"10.2174\/1389557519666190913151632","article-title":"Natural polysaccharides with immunomodulatory activities","volume":"20","author":"Zhao","year":"2020","journal-title":"Mini Rev. Med. Chem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/j.carbpol.2016.04.097","article-title":"Toll-like receptor 4-related immunostimulatory polysaccharides: Primary structure, activity relationships, and possible interaction models","volume":"149","author":"Zhang","year":"2016","journal-title":"Carbohydr. Polym."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"7005","DOI":"10.1128\/IAI.72.12.7005-7011.2004","article-title":"Immunostimulating properties of intragastrically administered Acetobacter-derived soluble branched (1, 4)-\u03b2-D-glucans decrease murine susceptibility to Listeria monocytogenes","volume":"72","author":"Li","year":"2004","journal-title":"Infect. Immun."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"7168231","DOI":"10.1155\/2018\/7168231","article-title":"D-Mannose enhanced immunomodulation of Periodontal Ligament stem cells via inhibiting IL-6 secretion","volume":"2018","author":"Guo","year":"2018","journal-title":"Stem Cells Int."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"104579","DOI":"10.1016\/j.jff.2021.104579","article-title":"Immunomodulatory activity of polysaccharide from Arca granosa Linnaeus via TLR4\/MyD88\/NF\u03baB and TLR4\/TRIF signaling pathways","volume":"84","author":"Zhu","year":"2021","journal-title":"J. Funct. Foods"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"858","DOI":"10.1016\/j.intimp.2013.09.019","article-title":"Purification, characterization and immunomodulating activity of a pectic polysaccharide isolated from Korean mulberry fruit Oddi (Morus alba L.)","volume":"17","author":"Lee","year":"2013","journal-title":"Int. Immunopharmacol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1007\/s00424-009-0765-9","article-title":"Cellular signaling and NO production","volume":"459","author":"Michel","year":"2010","journal-title":"Pflug. Arch. Eur. J. Phy."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1016\/j.procbio.2016.01.003","article-title":"Immunomodulatory effects of a polysaccharide purified from Lepidium meyenii Walp. on macrophages","volume":"51","author":"Wang","year":"2016","journal-title":"Process Biochem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1016\/j.ijbiomac.2017.02.028","article-title":"Immunomodulatory effects of an acetylated Cyclocarya paliurus polysaccharide on murine macrophages RAW264. 7","volume":"98","author":"Liu","year":"2017","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"127257","DOI":"10.1016\/j.foodchem.2020.127257","article-title":"Immunomodulatory activity of a novel polysaccharide extracted from Huangshui on THP-1 cells through NO production and increased IL-6 and TNF-\u03b1 expression","volume":"330","author":"Huo","year":"2020","journal-title":"Food Chem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1016\/j.carbpol.2014.12.023","article-title":"Activation of RAW264. 7 macrophages by the polysaccharide from the roots of Actinidia eriantha and its molecular mechanisms","volume":"121","author":"Sun","year":"2015","journal-title":"Carbohyd. Polym."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1007\/s10142-023-01180-3","article-title":"RNA-seq analysis reveals an immunomodulatory peptide from highland barley activating RAW264. 7 macrophages via TNF\/NF-\u03baB signaling pathway","volume":"23","author":"Liu","year":"2023","journal-title":"Funct. Integr. Genomic."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1670","DOI":"10.3390\/cancers6031670","article-title":"Tumor-associated macrophages as major players in the tumor microenvironment","volume":"6","author":"Chanmee","year":"2014","journal-title":"Cancers"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/j.ijbiomac.2016.05.062","article-title":"Assessment of biochemical and immunomodulatory activity of sulphated polysaccharides from Ulva intestinalis","volume":"91","author":"Peasura","year":"2016","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.carbpol.2017.02.032","article-title":"In vitro and in vivo immunomodulatory activity of sulfated polysaccharide from Porphyra haitanensis","volume":"165","author":"Liu","year":"2017","journal-title":"Carbohydr. Polym."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"37952","DOI":"10.1039\/D1RA07214E","article-title":"Physicochemical, morpho-structural, and biological characterization of polysaccharides from three Polygonatum spp.","volume":"11","author":"Bai","year":"2021","journal-title":"RSC Adv."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1146\/annurev.immunol.19.1.683","article-title":"Interleukin-10 and the Interleukin-10 Receptor","volume":"19","author":"Moore","year":"2001","journal-title":"Annu. Rev. Immunol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/S1359-6101(02)00012-6","article-title":"The family of IL-10-related cytokines and their receptors: Related, but to what extent?","volume":"13","author":"Kotenko","year":"2002","journal-title":"Cytokine Growth Factor Rev."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"23337","DOI":"10.1002\/jcp.28901","article-title":"Immunomodulatory activity of Ganoderma lucidum immunomodulatory protein via PI3K\/Akt and MAPK signaling pathways in RAW264.7 cells","volume":"234","author":"Li","year":"2019","journal-title":"J. Cell Physiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"85838","DOI":"10.18632\/oncotarget.20689","article-title":"Extracts from Hericium erinaceus relieve inflammatory bowel disease by regulating immunity and gut microbiota","volume":"8","author":"Diling","year":"2017","journal-title":"Oncotarget"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"341","DOI":"10.2147\/JIR.S292244","article-title":"Iridin revented against lipopolysaccharide-induced inflammatory responses of macrophages via inactivation of PKM2-mediated glycolytic pathways","volume":"14","author":"Ying","year":"2021","journal-title":"J. Inflamm. Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1186\/s12931-018-0777-0","article-title":"Human alveolar macrophages predominately express combined classical M1 and M2 surface markers in steady state","volume":"19","author":"Mitsi","year":"2018","journal-title":"Respir. Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"6425","DOI":"10.1002\/jcp.26429","article-title":"Macrophage plasticity, polarization, and function in health and disease","volume":"233","author":"Mohammadian","year":"2018","journal-title":"J. Cell. Physiol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.jss.2015.03.023","article-title":"M1 to M2 macrophage polarization in heparin-binding epidermal growth factor-like growth factor therapy for necrotizing enterocolitis","volume":"197","author":"Wei","year":"2015","journal-title":"J. Surg. Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1016\/j.jep.2012.05.055","article-title":"Modulatory effects of the acid polysaccharide fraction from one of anamorph of Cordyceps sinensis on Ana-1 cells","volume":"142","author":"Chen","year":"2012","journal-title":"J. Ethnopharmacol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/j.ijbiomac.2020.02.050","article-title":"Cordyceps militaris polysaccharide converts immunosuppressive macrophages into M1-like phenotype and activates T lymphocytes by inhibiting the PD-L1\/PD-1 axis between TAMs and T lymphocytes","volume":"150","author":"Bi","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1039\/C7FO01147D","article-title":"Structural elucidation and immunostimulatory activity of a new polysaccharide from Cordyceps militaris","volume":"9","author":"Bi","year":"2018","journal-title":"Food Funct."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1038\/s41392-021-00687-0","article-title":"Pattern recognition receptors in health and diseases","volume":"6","author":"Li","year":"2021","journal-title":"Signal Transduct. Tar."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1038\/nri2035","article-title":"MAPK phosphatases-regulating the immune response","volume":"7","author":"Liu","year":"2007","journal-title":"Nat. Rev. Immunol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1002\/iub.1625","article-title":"RNA sensors of the innate immune system and their detection of pathogens","volume":"69","author":"Chen","year":"2017","journal-title":"IUBMB Life"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"5989","DOI":"10.4049\/jimmunol.173.10.5989","article-title":"Extract of reishi polysaccharides induces cytokine expression via TLR4-modulated protein kinase signaling pathways","volume":"173","author":"Hsu","year":"2004","journal-title":"J. Immunol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1053\/rvsc.1998.0237","article-title":"Use of lipopolysaccharide (LPS) as a positive control for the evaluation of immunopotentiating drug candidates in experimental avian colibacillosis models","volume":"66","author":"McGruder","year":"1999","journal-title":"Res. Vet. Sci."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.ab.2004.12.001","article-title":"Carbohydrate analysis by a phenol\u2013sulfuric acid method in microplate format","volume":"339","author":"Masuko","year":"2005","journal-title":"Anal. Biochem."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1344523","DOI":"10.1080\/16546628.2017.1344523","article-title":"Polysaccharide from wheat bran induces cytokine expression via the toll-like receptor 4-mediated p38 MAPK signaling pathway and prevents cyclophosphamide-induced immunosuppression in mice","volume":"61","author":"Shen","year":"2017","journal-title":"Food Nutr. Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1007\/s00011-019-01313-0","article-title":"Aloperine suppresses LPS-induced macrophage activation through inhibiting the TLR4\/NF-\u03baB pathway","volume":"69","author":"Ye","year":"2020","journal-title":"Inflamm. Res."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Lu, J., Chen, X., Xu, X., Liu, J., Zhang, Z., Wang, M., Li, X., Chen, H., Zhao, D., and Wang, J. (2019). Active polypeptides from Hirudo inhibit endothelial cell inflammation and macrophage foam cell formation by regulating the LOX-1\/LXR-\u03b1\/ABCA1 pathway. Biomed. Pharmacother., 115.","DOI":"10.1016\/j.biopha.2019.108840"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1016\/j.ijbiomac.2021.08.053","article-title":"Characterization of a neutral polysaccharide from pumpkin (Cucurbita moschata Duch) with potential immunomodulatory activity","volume":"188","author":"Huang","year":"2021","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/j.carbpol.2019.03.003","article-title":"A polysaccharide isolated and purified from Platycladus orientalis (L.) Franco leaves, characterization, bioactivity and its regulation on macrophage polarization","volume":"213","author":"Ren","year":"2019","journal-title":"Carbohydr. Polym."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"152864","DOI":"10.1016\/j.phymed.2019.152864","article-title":"The in vitro and in vivo anti-inflammatory effect of osthole, the major natural coumarin from Cnidium monnieri (L.) Cuss, via the blocking of the activation of the NF-\u03baB and MAPK\/p38 pathways","volume":"58","author":"Fan","year":"2019","journal-title":"Phytomedicine"}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/29\/14\/3408\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:20:16Z","timestamp":1760109616000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/29\/14\/3408"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,20]]},"references-count":53,"journal-issue":{"issue":"14","published-online":{"date-parts":[[2024,7]]}},"alternative-id":["molecules29143408"],"URL":"https:\/\/doi.org\/10.3390\/molecules29143408","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,20]]}}}