{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T02:08:45Z","timestamp":1781316525219,"version":"3.54.1"},"reference-count":33,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2024,7,24]],"date-time":"2024-07-24T00:00:00Z","timestamp":1721779200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100014219","name":"National Science Fund for Distinguished Young Scholars","doi-asserted-by":"publisher","award":["82003888"],"award-info":[{"award-number":["82003888"]}],"id":[{"id":"10.13039\/501100014219","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014219","name":"National Science Fund for Distinguished Young Scholars","doi-asserted-by":"publisher","award":["QJRC2022055"],"award-info":[{"award-number":["QJRC2022055"]}],"id":[{"id":"10.13039\/501100014219","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Chengdu University of Traditional Chinese Medicine Youth Talent Program","award":["82003888"],"award-info":[{"award-number":["82003888"]}]},{"name":"Chengdu University of Traditional Chinese Medicine Youth Talent Program","award":["QJRC2022055"],"award-info":[{"award-number":["QJRC2022055"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Previous studies have indicated that there may be differences among the varieties of lemon flavonoids, but the details have not yet been made clear, which limits the comprehensive use of different cultivated lemon varieties. In this study, ultra-performance liquid chromatography\u2013quadrupole\u2013time-of-flight\u2013mass spectrometry (UPLC\u2013Q\u2013TOF\u2013MS) and ultraviolet\u2013visible spectroscopy (UV\u2013Vis) were used to investigate the types and contents of flavonoids in the flesh of the main cultivated variety (Eureka) and five common lemon varieties, as well as their in vitro antioxidant activity. A total of 21 compounds were identified, five of which were common compounds. Among them, Verna, Lisbon, and Bearss each have characteristic components that can serve as potential criteria for variety identification. Each of the six varieties of lemon has strong antioxidant activity. The antioxidant activity of different lemon varieties is related to flavonoids. Therefore, Eureka and the other five varieties of lemon are good natural antioxidants, and the cultivation and industrial production of lemons should consider the needs and selection of suitable varieties.<\/jats:p>","DOI":"10.3390\/molecules29153464","type":"journal-article","created":{"date-parts":[[2024,7,24]],"date-time":"2024-07-24T10:46:20Z","timestamp":1721817980000},"page":"3464","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Flavonoid Profiles in the Pulp of Different Lemon Cultivars and Their Antioxidant Activity Based on UPLC\u2013Q\u2013TOF\u2013MS"],"prefix":"10.3390","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2586-5273","authenticated-orcid":false,"given":"Zhixiang","family":"Liu","sequence":"first","affiliation":[{"name":"Institute of Herbgenomics, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengcheng","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Herbgenomics, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Tang","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of the Innovative Chinese Materia Medica & Health Intervention, Chongqing Academy of Chinese Materia Medica, Chongqing College of Traditional Chinese Medicine, Chongqing 400065, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1186\/s41021-020-00156-0","article-title":"Isolation and structure elucidation of constituents of Citrus limon, Isodon japonicus, and Lansium domesticum as the cancer prevention agents","volume":"42","author":"Matsumoto","year":"2020","journal-title":"Genes Environ."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"uhad020","DOI":"10.1093\/hr\/uhad020","article-title":"A gap-free and haplotype-resolved lemon genome provides insights into flavor synthesis and huanglongbing (HLB) tolerance","volume":"10","author":"Bao","year":"2023","journal-title":"Hortic. Res."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1038\/nature25447","article-title":"Genomics of the origin and evolution of Citrus","volume":"554","author":"Wu","year":"2018","journal-title":"Nature"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1964","DOI":"10.1038\/s41588-023-01516-6","article-title":"Pangenome analysis provides insight into the evolution of the orange subfamily and a key gene for citric acid accumulation in citrus fruits","volume":"55","author":"Huang","year":"2023","journal-title":"Nat. Genet."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"130585","DOI":"10.1016\/j.foodchem.2021.130585","article-title":"A review of chemical constituents and health-promoting effects of citrus peels","volume":"365","author":"Liu","year":"2021","journal-title":"Food Chem."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"3852","DOI":"10.1002\/fsn3.2363","article-title":"Application of graphene oxide in the adsorption and extraction of bioactive compounds from lemon peel","volume":"9","author":"Nasirian","year":"2021","journal-title":"Food Sci. Nutr."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1080\/03601234.2022.2046993","article-title":"Green-sustainable extraction techniques for the recovery of antioxidant compounds from \u201cCitrus limon\u201d by-products","volume":"57","author":"Imeneo","year":"2022","journal-title":"J. Environ. Sci. Health B"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"111252","DOI":"10.1016\/j.foodres.2022.111252","article-title":"Integration of pressurized liquid extraction and in-line solid-phase extraction to simultaneously extract and concentrate phenolic compounds from lemon peel (Citrus limon L.)","volume":"157","author":"Chaves","year":"2022","journal-title":"Food Res. Int."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Ou, Q., Zhao, J., Sun, Y., Zhao, Y., and Zhang, B. (2023). Utilization of Lemon Peel for the Production of Vinegar by a Combination of Alcoholic and Acetic Fermentations. Foods, 12.","DOI":"10.3390\/foods12132488"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"S\u00e1nchez-Bravo, P., Mart\u00ednez-Tom\u00e9, J., Hern\u00e1ndez, F., Sendra, E., and Noguera-Artiaga, L. (2023). Conventional vs. Organic: Evaluation of Nutritional, Functional and Sensory Quality of Citrus limon. Foods, 12.","DOI":"10.3390\/foods12234304"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"125124","DOI":"10.1016\/j.foodchem.2019.125124","article-title":"Anti-inflammatory effects of flavonoids","volume":"299","author":"Maleki","year":"2019","journal-title":"Food Chem."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Klimek-Szczykutowicz, M., Szopa, A., and Ekiert, H. (2020). Citrus limon (Lemon) Phenomenon-A Review of the Chemistry, Pharmacological Properties, Applications in the Modern Pharmaceutical, Food, and Cosmetics Industries, and Biotechnological Studies. Plants, 9.","DOI":"10.3390\/plants9010119"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Park, Y.S., Kim, I.D., Dhungana, S.K., Park, E.J., Park, J.J., Kim, J.H., and Shin, D.H. (2021). Quality Characteristics and Antioxidant Potential of Lemon (Citrus limon Burm. f.) Seed Oil Extracted by Different Methods. Front. Nutr., 8.","DOI":"10.3389\/fnut.2021.644406"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"100368","DOI":"10.1016\/j.fochx.2022.100368","article-title":"The flavonoid profiles in the pulp of different pomelo (Citrus grandis L. Osbeck) and grapefruit (Citrus paradisi Mcfad) cultivars and their in vitro bioactivity","volume":"15","author":"Deng","year":"2022","journal-title":"Food Chem. X"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"101197","DOI":"10.1016\/j.fochx.2024.101197","article-title":"Insights into metabolic characteristics and biological activity changes in Zangju (Citrus reticulata cv. Manau Gan) peel at different maturity stages through UPLC-MS\/MS-based metabolomics","volume":"21","author":"Wang","year":"2024","journal-title":"Food Chem. X"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.1016\/j.foodchem.2012.09.085","article-title":"Phenolic profiles and antioxidant activity of litchi pulp of different cultivars cultivated in Southern China","volume":"136","author":"Zhang","year":"2013","journal-title":"Food Chem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"130413","DOI":"10.1016\/j.foodchem.2021.130413","article-title":"Evaluation of phenolic composition and antioxidant properties of different varieties of Chinese citrus","volume":"364","author":"Chen","year":"2021","journal-title":"Food Chem."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1108","DOI":"10.1007\/s13197-017-2544-5","article-title":"Characterization of phenolic profile and antioxidant capacity of different fruit part from lemon (Citrus limon Burm.) cultivars","volume":"54","author":"Xi","year":"2017","journal-title":"J. Food Sci. Technol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"127240","DOI":"10.1016\/j.foodchem.2020.127240","article-title":"Phenolic profiling and evaluation of in vitro antioxidant, alpha-glucosidase and alpha-amylase inhibitory activities of Lepisanthes fruticosa (Roxb) Leenh fruit extracts","volume":"331","author":"Salahuddin","year":"2020","journal-title":"Food Chem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1007\/s13197-020-04524-0","article-title":"Influence of drying techniques on bioactive properties, phenolic compounds and fatty acid compositions of dried lemon and orange peel powders","volume":"58","author":"Ghafoor","year":"2021","journal-title":"J. Food Sci. Technol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"114712","DOI":"10.1016\/j.lwt.2023.114712","article-title":"The flavonoid profiles in different tissue parts of Shatianyu (Citrus grandis L. Osbeck) and their in vitro bioactivity","volume":"180","author":"Deng","year":"2023","journal-title":"LWT"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2026","DOI":"10.1055\/s-0030-1250542","article-title":"Metabolomics: Towards understanding traditional Chinese medicine","volume":"76","author":"Zhang","year":"2010","journal-title":"Planta Med."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"111486","DOI":"10.1016\/j.foodres.2022.111486","article-title":"A comparative metabolomic investigation in fruit sections of Citrus medica L. and Citrus maxima L. detecting potential bioactive metabolites using UHPLC-QTOF-IMS","volume":"157","author":"Dadwal","year":"2022","journal-title":"Food Res. Int."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"113117","DOI":"10.1016\/j.foodres.2023.113117","article-title":"Exploring the roles of microorganisms and metabolites in the 30-year aging process of the dried pericarps of Citrus reticulata \u2018Chachi\u2019 based on high-throughput sequencing and comparative metabolomics","volume":"172","author":"Wang","year":"2023","journal-title":"Food Res. Int."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Yan, H., Pu, Z.-J., Zhang, Z.-Y., Zhou, G.-S., Zou, D.-Q., Guo, S., Li, C., Zhan, Z.-L., and Duan, J.-A. (2021). Research on Biomarkers of Different Growth Periods and Different Drying Processes of Citrus wilsonii Tanaka Based on Plant Metabolomics. Front. Plant Sci., 12.","DOI":"10.3389\/fpls.2021.700367"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"10593","DOI":"10.1021\/acs.jafc.2c03515","article-title":"Natural Sweeteners and Sweetness-Enhancing Compounds Identified in Citrus Using an Efficient Metabolomics-Based Screening Strategy","volume":"70","author":"Wang","year":"2022","journal-title":"J. Agric. Food Chem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"100523","DOI":"10.1016\/j.crfs.2023.100523","article-title":"UPLC-Q-TOF\/MS-based metabolomic analysis reveals the effects of asomate on the citrus fruit","volume":"6","author":"He","year":"2023","journal-title":"Curr. Res. Food Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/j.foodchem.2019.05.136","article-title":"Citrus peels waste as a source of value-added compounds: Extraction and quantification of bioactive polyphenols","volume":"295","author":"Madrid","year":"2019","journal-title":"Food Chem."},{"key":"ref_29","unstructured":"National Pharmacopoeia Commission (2020). Pharmacopoeia of People\u2019s Republic of China, China Medical Science Press."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"109114","DOI":"10.1016\/j.foodres.2020.109114","article-title":"Phenolic composition, antioxidant potential and health benefits of citrus peel","volume":"132","author":"Singh","year":"2020","journal-title":"Food Res. Int."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1196","DOI":"10.1002\/jms.3637","article-title":"Characterization of lemon (Citrus limon) polar extract by liquid chromatography-tandem mass spectrometry in high resolution mode","volume":"50","year":"2015","journal-title":"J. Mass Spectrom."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"3010","DOI":"10.1021\/jf0115589","article-title":"Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity","volume":"50","author":"Dewanto","year":"2002","journal-title":"J. Agric. Food Chem."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1016\/j.ijbiomac.2017.02.044","article-title":"Constituent analysis of the ethanol extracts of Chimonanthus nitens Oliv. leaves and their inhibitory effect on alpha-glucosidase activity","volume":"98","author":"Chen","year":"2017","journal-title":"Int. J. Biol. Macromol."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/29\/15\/3464\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:22:24Z","timestamp":1760109744000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/29\/15\/3464"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,24]]},"references-count":33,"journal-issue":{"issue":"15","published-online":{"date-parts":[[2024,8]]}},"alternative-id":["molecules29153464"],"URL":"https:\/\/doi.org\/10.3390\/molecules29153464","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,24]]}}}