{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T18:48:04Z","timestamp":1787510884904,"version":"build-2736575974"},"reference-count":46,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2024,8,5]],"date-time":"2024-08-05T00:00:00Z","timestamp":1722816000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["32270424"],"award-info":[{"award-number":["32270424"]}]},{"name":"National Natural Science Foundation of China","award":["2022xkjT045"],"award-info":[{"award-number":["2022xkjT045"]}]},{"name":"National Natural Science Foundation of China","award":["KJ2020A0775"],"award-info":[{"award-number":["KJ2020A0775"]}]},{"name":"National Natural Science Foundation of China","award":["KJ2019A1301"],"award-info":[{"award-number":["KJ2019A1301"]}]},{"name":"Scientific Research Platform Improvement Project of Anhui Medical University","award":["32270424"],"award-info":[{"award-number":["32270424"]}]},{"name":"Scientific Research Platform Improvement Project of Anhui Medical University","award":["2022xkjT045"],"award-info":[{"award-number":["2022xkjT045"]}]},{"name":"Scientific Research Platform Improvement Project of Anhui Medical University","award":["KJ2020A0775"],"award-info":[{"award-number":["KJ2020A0775"]}]},{"name":"Scientific Research Platform Improvement Project of Anhui Medical University","award":["KJ2019A1301"],"award-info":[{"award-number":["KJ2019A1301"]}]},{"name":"University natural science research project of Anhui province","award":["32270424"],"award-info":[{"award-number":["32270424"]}]},{"name":"University natural science research project of Anhui province","award":["2022xkjT045"],"award-info":[{"award-number":["2022xkjT045"]}]},{"name":"University natural science research project of Anhui province","award":["KJ2020A0775"],"award-info":[{"award-number":["KJ2020A0775"]}]},{"name":"University natural science research project of Anhui province","award":["KJ2019A1301"],"award-info":[{"award-number":["KJ2019A1301"]}]},{"name":"University natural science research project of Anhui province","award":["32270424"],"award-info":[{"award-number":["32270424"]}]},{"name":"University natural science research project of Anhui province","award":["2022xkjT045"],"award-info":[{"award-number":["2022xkjT045"]}]},{"name":"University natural science research project of Anhui province","award":["KJ2020A0775"],"award-info":[{"award-number":["KJ2020A0775"]}]},{"name":"University natural science research project of Anhui province","award":["KJ2019A1301"],"award-info":[{"award-number":["KJ2019A1301"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Hypericum beanii N. Robson, a perennial upright herb, predominantly inhabits temperate regions. This species has been utilized for the treatment of various inflammation-related diseases. One new xanthone 3,7-dihydroxy-1,6-dimethoxyxanthone (1) and twenty-three known xanthones (2\u201324) were isolated from the aerial parts of H. beanii. The structure of the new compound was determined based on high-resolution electrospray ionization mass spectroscopy (HR-ESIMS), nuclear magnetic resonance (NMR), Infrared Spectroscopy (IR), ultraviolet spectrophotometry (UV) spectroscopic data. The anti-inflammatory effects of all the isolates were assessed by measuring the inhibitory effect on nitric oxide (NO) production in LPS-stimulated RAW 264.7 macrophages. Compounds 3,4-dihydroxy-2-methoxyxanthone (15), 1,3,5,6-tetrahydroxyxanthone (19), and 1,3,6,7-tetrahydroxyxanthone (22) exhibited significant anti-inflammatory effects at a concentration of 10 \u03bcM with higher potency compared to the positive control quercetin. Furthermore, compounds 15, 19, and 22 reduced inducible NO synthase (iNOS), tumor necrosis factor alpha (TNF-\u03b1), interleukin-1\u03b2 (IL-1\u03b2), IL-6, and cyclooxygenase 2 (COX-2) mRNA expression in the LPS-stimulated RAW 264.7 macrophages, suggesting that these compounds may mitigate the synthesis of the aforementioned molecules at the transcriptional level, provisionally confirming their anti-inflammatory efficacy.<\/jats:p>","DOI":"10.3390\/molecules29153705","type":"journal-article","created":{"date-parts":[[2024,8,5]],"date-time":"2024-08-05T13:57:28Z","timestamp":1722866248000},"page":"3705","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Anti-Inflammatory Effect of Xanthones from Hypericum beanii on Macrophage RAW 264.7 Cells through Reduced NO Production and TNF-\u03b1, IL-1\u03b2, IL-6, and COX-2 Expression"],"prefix":"10.3390","volume":"29","author":[{"given":"Wei","family":"Ma","sequence":"first","affiliation":[{"name":"School of Pharmacy, Anhui Medical University, No.81 Meishan Road, Shushan District, Hefei 230032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8685-9283","authenticated-orcid":false,"given":"Fu-Cai","family":"Ren","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Anhui Medical University, No.81 Meishan Road, Shushan District, Hefei 230032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue-Ru","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Anhui Medical University, No.81 Meishan Road, Shushan District, Hefei 230032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2650-363X","authenticated-orcid":false,"given":"Ning","family":"Li","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Anhui Medical University, No.81 Meishan Road, Shushan District, Hefei 230032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Duan, Y.L., Deng, Y.F., Bu, P.F., Guo, Y., Shi, Z.Y., Cao, Y.Y., Zhang, Y.T., Hu, H., Qi, Z.X., and Hu, C.X. 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