{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T15:51:30Z","timestamp":1766159490758,"version":"build-2065373602"},"reference-count":36,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2024,8,18]],"date-time":"2024-08-18T00:00:00Z","timestamp":1723939200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Fund of Qiqihar Academy of Medical Sciences","award":["No.QMSI2022Z-02","LH2022H108","2021-KYYWF-0337"],"award-info":[{"award-number":["No.QMSI2022Z-02","LH2022H108","2021-KYYWF-0337"]}]},{"name":"the Joint Guidance Project of Natural Science Fund of Heilongjiang Province","award":["No.QMSI2022Z-02","LH2022H108","2021-KYYWF-0337"],"award-info":[{"award-number":["No.QMSI2022Z-02","LH2022H108","2021-KYYWF-0337"]}]},{"name":"the Excellent Innovation Team Project of Basic Scientific Research Business Expenses in Provincial Universities in Heilongjiang Province","award":["No.QMSI2022Z-02","LH2022H108","2021-KYYWF-0337"],"award-info":[{"award-number":["No.QMSI2022Z-02","LH2022H108","2021-KYYWF-0337"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The equilibrium state of hydrogen sulfide (H2S), a gaseous signaling molecule produced by lysosomal metabolites, in vivo is crucial for cellular function. Abnormal fluctuations in H2S concentration can interfere with the normal function of lysosomes, which has been closely linked to the pathogenesis of a variety of diseases. In view of this, a novel fluorescent probe Lyso-DPP based on 1,3,5-triarylpyrazolines was developed for the precise detection of H2S in lysosomes by using the hydrophilic morpholine moiety as a lysosomal targeting unit, and 2,4-dinitroanisole as a fluorescence-quenching and H2S-responsive unit. The probe cleverly combines the advantages of simple synthesis, sensitive blue fluorescence turn-on with a limit of detection, LOD, of 97.3 nM, good stability, and fast response time (10 min), which makes Lyso-DPP successful in portable monitoring of meat freshness in the form of test strips. Moreover, the excellent biocompatibility and precise targeting capability of the probe Lyso-DPP make it perform well in the monitoring of H2S in lysosomes, living cells, and zebrafish. This work not only provides new technical tools for food quality control but also paves up new ideas for early diagnosis and treatment of H2S-related diseases.<\/jats:p>","DOI":"10.3390\/molecules29163906","type":"journal-article","created":{"date-parts":[[2024,8,19]],"date-time":"2024-08-19T06:41:31Z","timestamp":1724049691000},"page":"3906","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Visual Tracking of Hydrogen Sulfide: Application of a Novel Lysosome-Targeted Fluorescent Probe for Bioimaging and Food Safety Assessment"],"prefix":"10.3390","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8912-244X","authenticated-orcid":false,"given":"Likun","family":"Liu","sequence":"first","affiliation":[{"name":"Research Institute of Medicine & Pharmacy, Qiqihar Medical University, Qiqihar 161006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yitong","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Qiqihar Medical University, Qiqihar 161006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haoqing","family":"Ren","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Qiqihar Medical University, Qiqihar 161006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peng","family":"Hou","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Qiqihar Medical University, Qiqihar 161006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haijun","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Qiqihar Medical University, Qiqihar 161006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingwen","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Qiqihar Medical University, Qiqihar 161006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Qiqihar Medical University, Qiqihar 161006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chuan","family":"He","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Qiqihar Medical University, Qiqihar 161006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Song","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Qiqihar Medical University, Qiqihar 161006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.saa.2017.10.044","article-title":"A lysosome-targetable turn-on fluorescent probe for the detection of thiols in living cells based on a 1,8-naphthalimide derivative","volume":"192","author":"Liang","year":"2018","journal-title":"Spectrochim Acta A"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"121966","DOI":"10.1016\/j.saa.2022.121966","article-title":"A novel near-infrared ratiometric fluorescent probe targeting lysosomes for imaging HOCl in vitro and in vivo","volume":"286","author":"Chen","year":"2023","journal-title":"Spectrochim Acta A"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"123987","DOI":"10.1016\/j.saa.2024.123987","article-title":"A novel lysosomal targeted near-infrared probe for ratio detection of carbon monoxide in cells and in vivo","volume":"311","author":"Ji","year":"2024","journal-title":"Spectrochim Acta A"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"124316","DOI":"10.1016\/j.saa.2024.124316","article-title":"A lysosome-targeted fluorescent probe for fluorescence imaging of hypochlorous acid in living cells and in vivo","volume":"316","author":"An","year":"2024","journal-title":"Spectrochim Acta A"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"341819","DOI":"10.1016\/j.aca.2023.341819","article-title":"Real-time monitoring of endogenous cysteine in LPS-induced oxidative stress process with a novel lysosome-targeted fluorescent probe","volume":"1279","author":"Yang","year":"2023","journal-title":"Anal. 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