{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T02:19:44Z","timestamp":1788401984642,"version":"build-2803163510"},"reference-count":53,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2024,8,22]],"date-time":"2024-08-22T00:00:00Z","timestamp":1724284800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"BMBF\/Forschungszentrum J\u00fclich GmbH","award":["2022000287\u2014KMUi-B\u00d602: EKO-YAM"],"award-info":[{"award-number":["2022000287\u2014KMUi-B\u00d602: EKO-YAM"]}]},{"name":"Freie Universit\u00e4t Berlin","award":["2022000287\u2014KMUi-B\u00d602: EKO-YAM"],"award-info":[{"award-number":["2022000287\u2014KMUi-B\u00d602: EKO-YAM"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The Chinese yam (Dioscorea polystachya, DP) is promising for the food and pharmaceutical industries due to its nutritional value and pharmaceutical potential. Its proper cultivation is therefore of interest. An insufficient supply of minerals necessary for plant growth can be manifested by discoloration of the leaves. In our earlier study, magnesium deficiency was excluded as a cause. As a follow-up, this work focused on manganese and molybdenum. To quantify both minerals in leaf extracts of DP, analytical methods based on atomic absorption spectrometry (AAS) using the graphite furnace sub-technique were devised. The development revealed that the quantification of manganese works best without using any of the investigated modifiers. The optimized pyrolysis and atomization temperatures were 1300 \u00b0C and 1800 \u00b0C, respectively. For the analysis of molybdenum, calcium proved to be advantageous as a modifier. The optimum temperatures were 1900 \u00b0C and 2800 \u00b0C, respectively. Both methods showed satisfactory linearity for analysis. Thus, they were applied to quantify extracts from normal and discolored leaves of DP concerning the two minerals. It was found that discolored leaves had higher manganese levels and a lower molybdenum content. With these results, a potential explanation for the discoloration could be found.<\/jats:p>","DOI":"10.3390\/molecules29163975","type":"journal-article","created":{"date-parts":[[2024,8,22]],"date-time":"2024-08-22T11:14:41Z","timestamp":1724325281000},"page":"3975","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Investigating the Discoloration of Leaves of Dioscorea polystachya Using Developed Atomic Absorption Spectrometry Methods for Manganese and Molybdenum"],"prefix":"10.3390","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-2129-9801","authenticated-orcid":false,"given":"David","family":"Kr\u00fcger","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Biology, Institute of Pharmacy, Freie Universit\u00e4t Berlin, K\u00f6nigin-Luise-Stra\u00dfe 2+4, 14195 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1640-2189","authenticated-orcid":false,"given":"Alexander","family":"Weng","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Biology, Institute of Pharmacy, Freie Universit\u00e4t Berlin, K\u00f6nigin-Luise-Stra\u00dfe 2+4, 14195 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1963-9838","authenticated-orcid":false,"given":"Daniel","family":"Baecker","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical and Medicinal Chemistry, Institute of Pharmacy, Freie Universit\u00e4t Berlin, K\u00f6nigin-Luise-Stra\u00dfe 2+4, 14195 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1007\/s00425-020-03458-3","article-title":"An underutilized orphan tuber crop\u2014Chinese yam: A review","volume":"252","author":"Epping","year":"2020","journal-title":"Planta"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.tifs.2023.01.021","article-title":"Chinese yam (Dioscorea): Nutritional value, beneficial effects, and food and pharmaceutical applications","volume":"134","author":"Li","year":"2023","journal-title":"Trends Food Sci. 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