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9237\/2015"],"award-info":[{"award-number":["FAPESB, Process No. 9237\/2015"]}]},{"name":"National Council for Scientific and Technological Development","award":["Ph.D. fellowship Process No. 084.0508.2020.0001112-18"],"award-info":[{"award-number":["Ph.D. fellowship Process No. 084.0508.2020.0001112-18"]}]},{"name":"National Council for Scientific and Technological Development","award":["CAPES, Process PGCI No. 88881.117666\/2016-01"],"award-info":[{"award-number":["CAPES, Process PGCI No. 88881.117666\/2016-01"]}]},{"name":"National Council for Scientific and Technological Development","award":["CAPES Process PGDI No. 88887.660190\/2021-00"],"award-info":[{"award-number":["CAPES Process PGDI No. 88887.660190\/2021-00"]}]},{"name":"National Council for Scientific and Technological Development","award":["Processes No. 312388\/2021-7"],"award-info":[{"award-number":["Processes No. 312388\/2021-7"]}]},{"name":"National Council for Scientific and Technological Development","award":["CNPq\/MCTI Process No. 407833\/2023-4"],"award-info":[{"award-number":["CNPq\/MCTI Process No. 407833\/2023-4"]}]},{"name":"National Institute for Translational Neuroscience Brazil","award":["FAPESB, Process No. 9237\/2015"],"award-info":[{"award-number":["FAPESB, Process No. 9237\/2015"]}]},{"name":"National Institute for Translational Neuroscience Brazil","award":["Ph.D. fellowship Process No. 084.0508.2020.0001112-18"],"award-info":[{"award-number":["Ph.D. fellowship Process No. 084.0508.2020.0001112-18"]}]},{"name":"National Institute for Translational Neuroscience Brazil","award":["CAPES, Process PGCI No. 88881.117666\/2016-01"],"award-info":[{"award-number":["CAPES, Process PGCI No. 88881.117666\/2016-01"]}]},{"name":"National Institute for Translational Neuroscience Brazil","award":["CAPES Process PGDI No. 88887.660190\/2021-00"],"award-info":[{"award-number":["CAPES Process PGDI No. 88887.660190\/2021-00"]}]},{"name":"National Institute for Translational Neuroscience Brazil","award":["Processes No. 312388\/2021-7"],"award-info":[{"award-number":["Processes No. 312388\/2021-7"]}]},{"name":"National Institute for Translational Neuroscience Brazil","award":["CNPq\/MCTI Process No. 407833\/2023-4"],"award-info":[{"award-number":["CNPq\/MCTI Process No. 407833\/2023-4"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Agathisflavone is a flavonoid that exhibits anti-inflammatory and anti-oxidative properties. Here, we investigated the neuroprotective effects of agathisflavone on central nervous system (CNS) neurons and glia in the cerebellar slice ex vivo model of neonatal ischemia. Cerebellar slices from neonatal mice, in which glial fibrillary acidic protein (GFAP) and SOX10 drive expression of enhanced green fluorescent protein (EGFP), were used to identify astrocytes and oligodendrocytes, respectively. Agathisflavone (10 \u03bcM) was administered preventively for 60 min before inducing ischemia by oxygen and glucose deprivation (OGD) for 60 min and compared to controls maintained in normal oxygen and glucose (OGN). The density of SOX-10+ oligodendrocyte lineage cells and NG2 immunopositive oligodendrocyte progenitor cells (OPCs) were not altered in OGD, but it resulted in significant oligodendroglial cell atrophy marked by the retraction of their processes, and this was prevented by agathisflavone. OGD caused marked axonal demyelination, determined by myelin basic protein (MBP) and neurofilament (NF70) immunofluorescence, and this was blocked by agathisflavone preventative treatment. OGD also resulted in astrocyte reactivity, exhibited by increased GFAP-EGFP fluorescence and decreased expression of glutamate synthetase (GS), and this was prevented by agathisflavone pretreatment. In addition, agathisflavone protected Purkinje neurons from ischemic damage, assessed by calbindin (CB) immunofluorescence. The results demonstrate that agathisflavone protects neuronal and myelin integrity in ischemia, which is associated with the modulation of glial responses in the face of ischemic damage.<\/jats:p>","DOI":"10.3390\/molecules29174159","type":"journal-article","created":{"date-parts":[[2024,9,2]],"date-time":"2024-09-02T10:07:35Z","timestamp":1725271655000},"page":"4159","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Neuroprotective Effect of Flavonoid Agathisflavone in the Ex Vivo Cerebellar Slice Neonatal Ischemia"],"prefix":"10.3390","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6947-1060","authenticated-orcid":false,"given":"Rodrigo Barreto","family":"Carreira","sequence":"first","affiliation":[{"name":"Laboratory of Neurochemistry and Cellular Biology, Institute of Health Sciences, Federal University of Bahia, Av. Reitor Miguel Calmon S\/N, Salvador 40231-300, BA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cleonice Creusa","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Laboratory of Neurochemistry and Cellular Biology, Institute of Health Sciences, Federal University of Bahia, Av. Reitor Miguel Calmon S\/N, Salvador 40231-300, BA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juciele Valeria Ribeiro","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Laboratory of Neurochemistry and Cellular Biology, Institute of Health Sciences, Federal University of Bahia, Av. Reitor Miguel Calmon S\/N, Salvador 40231-300, BA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8032-9663","authenticated-orcid":false,"given":"Victor Diogenes Amaral","family":"da Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Neurochemistry and Cellular Biology, Institute of Health Sciences, Federal University of Bahia, Av. Reitor Miguel Calmon S\/N, Salvador 40231-300, BA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6375-9055","authenticated-orcid":false,"given":"Jorge Maur\u00edcio","family":"David","sequence":"additional","affiliation":[{"name":"Department of General and Inorganic Chemistry, Institute of Chemistry, University Federal da Bahia, Salvador 40170-110, BA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7579-0746","authenticated-orcid":false,"given":"Arthur Morgan","family":"Butt","sequence":"additional","affiliation":[{"name":"School of Medicine, Pharmacy and Biomedical Sciences, University of Portsmouth, Portsmouth PO1 2DT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8975-3871","authenticated-orcid":false,"given":"Silvia Lima","family":"Costa","sequence":"additional","affiliation":[{"name":"Laboratory of Neurochemistry and Cellular Biology, Institute of Health Sciences, Federal University of Bahia, Av. Reitor Miguel Calmon S\/N, Salvador 40231-300, BA, Brazil"},{"name":"National Institute of Translational Neuroscience (INNT), Rio de Janeiro 21941-902, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1161\/CIRCRESAHA.116.308427","article-title":"Cerebral Vascular Disease and Neurovascular Injury in Ischemic Stroke","volume":"120","author":"Hu","year":"2017","journal-title":"Circ. Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1016\/S1474-4422(18)30499-X","article-title":"Global, regional, and national burden of neurological disorders, 1990\u20132016: A systematic analysis for the Global Burden of Disease Study 2016","volume":"18","author":"Feigin","year":"2019","journal-title":"Lancet Neurol."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Verkhratsky, A., and Butt, A.M. 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