{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T19:05:15Z","timestamp":1790795115802,"version":"4.1.0"},"reference-count":57,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2024,9,5]],"date-time":"2024-09-05T00:00:00Z","timestamp":1725494400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"R\u00e9gion Centre Val de Loire"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Accessing plant resources to extract compounds of interest can sometimes be challenging. To facilitate access and limit the environmental impact, innovative cultivation strategies can be developed. Forskolin is a molecule of high interest, mainly found in the roots of Coleus forskohlii. The aim of this study was to develop aeroponic cultivation methods to provide a local source of Coleus forskohlii and to study the impact of abiotic stress on forskolin and bioactive metabolite production. Three cultivation itineraries (LED lighting, biostimulant, and hydric stress) along with a control itinerary were established. The forskolin content in the plant roots was quantified using HPLC-ELSD, and the results showed that LED treatment proved to be the most promising, increasing root biomass and the total forskolin content recovered at the end of the cultivation period threefold (710.1 \u00b1 21.3 mg vs. 229.9 \u00b1 17.7 mg). Statistical analysis comparing the LED itinerary to the control itinerary identified stress-affected metabolites, showing that LEDs positively influence mainly the concentration of phenolic compounds in the roots and diterpenes in the aerial parts of Coleus forskohlii. Moreover, to better define the phytochemical composition of Coleus forskohlii cultivated in France using aeroponic cultivation, an untargeted metabolomic analysis was conducted using UHPLC-HRMS\/MS analysis and molecular networks on both the root and aerial parts. This study demonstrates that aeroponic cultivation, especially with the application of an LED treatment, could be a very promising alternative for a local source of Coleus forskohlii leading to easy access to the roots and aerial parts rich in forskolin and other bioactive compounds.<\/jats:p>","DOI":"10.3390\/molecules29174215","type":"journal-article","created":{"date-parts":[[2024,9,5]],"date-time":"2024-09-05T06:44:29Z","timestamp":1725518669000},"page":"4215","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Enhancement of Forskolin Production Using Aeroponic Cultivation of Coleus forskohlii and the Impact on the Plant Phytochemistry"],"prefix":"10.3390","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-4611-2029","authenticated-orcid":false,"given":"Audrey","family":"Le Cabec","sequence":"first","affiliation":[{"name":"Institut de Chimie Organique et Analytique (ICOA), Universit\u00e9 d\u2019Orl\u00e9ans, CNRSUMR 7311, 45067 Orleans, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5410-1606","authenticated-orcid":false,"given":"Pierre-Eric","family":"Campos","sequence":"additional","affiliation":[{"name":"Institut de Chimie Organique et Analytique (ICOA), Universit\u00e9 d\u2019Orl\u00e9ans, CNRSUMR 7311, 45067 Orleans, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Yzebe","sequence":"additional","affiliation":[{"name":"Comit\u00e9 de D\u00e9veloppement Horticole R\u00e9gion Centre (CDHRC), 45590 St-Cyr-en-Val, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronan","family":"Pel\u00e9","sequence":"additional","affiliation":[{"name":"Comit\u00e9 de D\u00e9veloppement Horticole R\u00e9gion Centre (CDHRC), 45590 St-Cyr-en-Val, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1651-6813","authenticated-orcid":false,"given":"Cyril","family":"Colas","sequence":"additional","affiliation":[{"name":"Institut de Chimie Organique et Analytique (ICOA), Universit\u00e9 d\u2019Orl\u00e9ans, CNRSUMR 7311, 45067 Orleans, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7461-5046","authenticated-orcid":false,"given":"Emilie","family":"Destandau","sequence":"additional","affiliation":[{"name":"Institut de Chimie Organique et Analytique (ICOA), Universit\u00e9 d\u2019Orl\u00e9ans, CNRSUMR 7311, 45067 Orleans, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1007\/s13596-014-0169-z","article-title":"Ethnopharmacological and Phytomedical Knowledge of Coleus Forskohlii: An Approach towards Its Safety and Therapeutic Value","volume":"14","author":"Shivaprasad","year":"2014","journal-title":"Orient. 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