{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T21:31:36Z","timestamp":1771536696175,"version":"3.50.1"},"reference-count":61,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2024,9,5]],"date-time":"2024-09-05T00:00:00Z","timestamp":1725494400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["31800283"],"award-info":[{"award-number":["31800283"]}]},{"name":"National Natural Science Foundation of China","award":["32270417"],"award-info":[{"award-number":["32270417"]}]},{"name":"National Natural Science Foundation of China","award":["202202AE090035"],"award-info":[{"award-number":["202202AE090035"]}]},{"name":"Science and Technology Project of Nujiang Prefecture, Yunnan Province, China","award":["31800283"],"award-info":[{"award-number":["31800283"]}]},{"name":"Science and Technology Project of Nujiang Prefecture, Yunnan Province, China","award":["32270417"],"award-info":[{"award-number":["32270417"]}]},{"name":"Science and Technology Project of Nujiang Prefecture, Yunnan Province, China","award":["202202AE090035"],"award-info":[{"award-number":["202202AE090035"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Many naturally occurring chemical metabolites with significant cytotoxic activities have been isolated from medicinal plants and have become the leading hotspot of anti-cancer research in recent years. Hyptis rhomboidea Mart. et Gal is used as a folk medicine in South China to treat or assist in the treatment of liver disease, ulcers, and edema. But its chemical constituents have not been fully investigated yet. This study aimed to assess the cytotoxicity of H. rhomboidea, which was chemically characterized by chromatography\u2013mass spectrometry methods. The results showed that the 95% ethanol extract of H. rhomboidea has marked inhibitory effects on five human cancer cell lines (HL-60, A549, SMMC-7721, MDA-MB-231, and SW480), with IC50 values ranging from 15.8 to 40.0 \u03bcg\/mL. A total of 64 compounds were identified by ultra-high-performance liquid chromatography with quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) and gas chromatograph\u2013mass spectroscopy (GC-MS) analysis of H. rhomboidea crude extract. Among them, kaempferol, quercetin, rosmarinic acid, squalene, and campesterol were found to be abundant and might be the major metabolites involved to its bioactivity. The cytotoxic characterization and metabolite profiling of H. rhomboidea displayed in this research provides scientific evidence to support its use as medicinal properties.<\/jats:p>","DOI":"10.3390\/molecules29174216","type":"journal-article","created":{"date-parts":[[2024,9,5]],"date-time":"2024-09-05T06:44:29Z","timestamp":1725518669000},"page":"4216","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The Cytotoxic Activity and Metabolic Profiling of Hyptis rhomboidea Mart. et Gal"],"prefix":"10.3390","volume":"29","author":[{"given":"Jian","family":"Zhang","sequence":"first","affiliation":[{"name":"Key Laboratory of Agro-Environment in the Tropics, College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenjie","family":"Gao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agro-Environment in the Tropics, College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2580-4876","authenticated-orcid":false,"given":"Israt","family":"Jahan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agro-Environment in the Tropics, College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Run","family":"Zhai","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agro-Environment in the Tropics, College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaiwei","family":"Yao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agro-Environment in the Tropics, College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8597-9841","authenticated-orcid":false,"given":"Jian","family":"Yan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agro-Environment in the Tropics, College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9161-8735","authenticated-orcid":false,"given":"Ping","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agro-Environment in the Tropics, College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"209","DOI":"10.3322\/caac.21660","article-title":"Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries","volume":"71","author":"Sung","year":"2021","journal-title":"CA Cancer J. 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