{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T18:03:00Z","timestamp":1792000980205,"version":"4.4.0"},"reference-count":46,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2024,9,5]],"date-time":"2024-09-05T00:00:00Z","timestamp":1725494400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012478","name":"Fundamental Research Funds of China West Normal University","doi-asserted-by":"publisher","award":["24kx010"],"award-info":[{"award-number":["24kx010"]}],"id":[{"id":"10.13039\/501100012478","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012478","name":"Fundamental Research Funds of China West Normal University","doi-asserted-by":"publisher","award":["SZK2212"],"award-info":[{"award-number":["SZK2212"]}],"id":[{"id":"10.13039\/501100012478","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012478","name":"Fundamental Research Funds of China West Normal University","doi-asserted-by":"publisher","award":["20E046"],"award-info":[{"award-number":["20E046"]}],"id":[{"id":"10.13039\/501100012478","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Panxi Crops Research and Utiliaztion Key Laboratory of Sichuan Province","award":["24kx010"],"award-info":[{"award-number":["24kx010"]}]},{"name":"Panxi Crops Research and Utiliaztion Key Laboratory of Sichuan Province","award":["SZK2212"],"award-info":[{"award-number":["SZK2212"]}]},{"name":"Panxi Crops Research and Utiliaztion Key Laboratory of Sichuan Province","award":["20E046"],"award-info":[{"award-number":["20E046"]}]},{"name":"Doctoral Scientific Research Foundation of China West Normal University","award":["24kx010"],"award-info":[{"award-number":["24kx010"]}]},{"name":"Doctoral Scientific Research Foundation of China West Normal University","award":["SZK2212"],"award-info":[{"award-number":["SZK2212"]}]},{"name":"Doctoral Scientific Research Foundation of China West Normal University","award":["20E046"],"award-info":[{"award-number":["20E046"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Chinese Olea europaea leaves, rich in verbascosides, were extracted using ultrasound-assisted extraction (UAE) and wall-breaking extraction (WBE) with deep eutectic solvents (Optimal UAE: 55 min, 200 mL\/g liquid\u2013solid ratio, 20% moisture, yielding 206.23 \u00b1 0.58 mg GAE\/g total phenolic content (TPC) and 1.59 \u00b1 0.04% verbascoside yield (VAY); Optimal WBE: 140 s, 210 mL\/g, 30% moisture, giving 210.69 \u00b1 0.97 mg GAE\/g TPC and 1.33 \u00b1 0.2% VAY). HPLC analysis showed that young leaves accumulated higher TPC and phenolic compounds. Among the five olive varieties, Koroneiki and Chemlal showed the highest TPC in UAE, while Arbosana and Chemlal excelled in WBE. WBE yielded a higher TPC and rutin, whereas UAE marginally increased other phenolics. Additionally, the DPPH\u2022 assay showed that WBE-extracted verbascoside-rich extracts (VREs) of Chemlal exhibited high antioxidant activity (EC50 of 57 mg\/mL), but Koroneiki-VREs exhibited lower activity against the ABTS\u2022+ radical (EC50 of 134 mg\/mL). Remarkably, the UAE\/WBE-extracted Chemlal-VREs promoted the normal esophageal Het-1A cell line at 25 \u03bcg\/mL for 24 h; yet, the esophageal cancer Eca-109 cells were sensibly inhibited, especially at 50 \u03bcg\/mL; and the cell viability decreased dramatically. The results confirmed WBE as a relatively efficient method, and the Chemlal variety may be an excellent source of verbascoside.<\/jats:p>","DOI":"10.3390\/molecules29174219","type":"journal-article","created":{"date-parts":[[2024,9,5]],"date-time":"2024-09-05T08:36:08Z","timestamp":1725525368000},"page":"4219","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Optimization of Different Extraction Methods for Phenolic Compound Verbascoside from Chinese Olea europaea Leaves Using Deep Eutectic Solvents: Impact on Antioxidant and Anticancer Activities"],"prefix":"10.3390","volume":"29","author":[{"given":"Yan","family":"Deng","sequence":"first","affiliation":[{"name":"College of Life Science, Environmental Science and Engineering, China West Normal University, Nanchong 637009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junlin","family":"Zhou","sequence":"additional","affiliation":[{"name":"College of Life Science, Environmental Science and Engineering, China West Normal University, Nanchong 637009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8674-1384","authenticated-orcid":false,"given":"Bixia","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Life Science, Environmental Science and Engineering, China West Normal University, Nanchong 637009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Life Science, Environmental Science and Engineering, China West Normal University, Nanchong 637009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingyu","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Life Science, Environmental Science and Engineering, China West Normal University, Nanchong 637009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhou","family":"Xu","sequence":"additional","affiliation":[{"name":"Panxi Crops Research and Utilization Key Laboratory of Sichuan Province, Xichang University, Xichang 615000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunyan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Sichuan Yizhang Agricultural Development Co., Ltd., Nanchong 637009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1007\/s11130-023-01131-0","article-title":"Exploring the pharmacological potential of Onosma riedliana: Phenolic compounds and their biological activities","volume":"79","author":"Sahinler","year":"2024","journal-title":"Plant Foods Hum. 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