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Province","award":["21326312D-3"],"award-info":[{"award-number":["21326312D-3"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Pinelliae Rhizoma (PR), a highly esteemed traditional Chinese medicinal herb, is widely applied in clinical settings due to its diverse pharmacological effects, including antitussive, expectorant, antiemetic, sedative-hypnotic, and antitumor activities. Pinellia ternata exhibits morphological variation in its leaves, with types resembling peach, bamboo, and willow leaves. However, the chemical composition differences among the corresponding rhizomes of these leaf phenotypes remain unelucidated. This pioneering research employed Matrix-Assisted Laser Desorption\/Ionization Mass Spectrometry Imaging (MALDI-MSI) to conduct the in situ identification and spatial profiling of 35 PR metabolites in PR, comprising 12 alkaloids, 4 organic acids, 12 amino acids, 5 flavonoids, 1 sterol, and 1 anthraquinone. Our findings revealed distinct spatial distribution patterns of secondary metabolites within the rhizome tissues of varying leaf types. Orthogonal Partial Least Squares Discriminant Analysis (OPLS-DA) effectively differentiated between rhizomes associated with different leaf morphologies. Furthermore, this study identified five potential differential biomarkers\u2014methylophiopogonanone B, inosine, cytidine, adenine, and leucine\/isoleucine\u2014that elucidate the biochemical distinctions among leaf types. The precise tissue-specific localization of these secondary metabolites offers compelling insights into the specialized accumulation of bioactive compounds in medicinal plants, thereby enhancing our comprehension of PR\u2019s therapeutic potential.<\/jats:p>","DOI":"10.3390\/molecules29174251","type":"journal-article","created":{"date-parts":[[2024,9,9]],"date-time":"2024-09-09T04:15:01Z","timestamp":1725855301000},"page":"4251","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Spatial Metabolomic Profiling of Pinelliae Rhizoma from Different Leaf Types Using Matrix-Assisted Laser Desorption\/Ionization Mass Spectrometry Imaging"],"prefix":"10.3390","volume":"29","author":[{"given":"Jiemin","family":"Wang","sequence":"first","affiliation":[{"name":"College of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang 050200, China"},{"name":"Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province, Shijiazhuang 050200, China"},{"name":"Key Laboratory for Quality Ensurance and Innovative TCMs of Dao-Di Herbs, Hebei Provincial Administration of Traditional Chinese Medicine, Shijiazhuang 050200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaowei","family":"Han","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang 050200, China"},{"name":"Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province, Shijiazhuang 050200, China"},{"name":"Key Laboratory for Quality Ensurance and Innovative TCMs of Dao-Di Herbs, Hebei Provincial Administration of Traditional Chinese Medicine, Shijiazhuang 050200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuguang","family":"Zheng","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang 050200, China"},{"name":"Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province, Shijiazhuang 050200, China"},{"name":"Key Laboratory for Quality Ensurance and Innovative TCMs of Dao-Di Herbs, Hebei Provincial Administration of Traditional Chinese Medicine, Shijiazhuang 050200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunsheng","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang 050200, China"},{"name":"Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province, Shijiazhuang 050200, China"},{"name":"Key Laboratory for Quality Ensurance and Innovative TCMs of Dao-Di Herbs, Hebei Provincial Administration of Traditional Chinese Medicine, Shijiazhuang 050200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenshuai","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang 050200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donglai","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang 050200, China"},{"name":"Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province, Shijiazhuang 050200, China"},{"name":"Key Laboratory for Quality Ensurance and Innovative TCMs of Dao-Di Herbs, Hebei Provincial Administration of Traditional Chinese Medicine, Shijiazhuang 050200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huigai","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang 050200, China"},{"name":"Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province, Shijiazhuang 050200, China"},{"name":"Key Laboratory for Quality Ensurance and Innovative TCMs of Dao-Di Herbs, Hebei Provincial Administration of Traditional Chinese Medicine, Shijiazhuang 050200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,7]]},"reference":[{"key":"ref_1","unstructured":"Chinese Pharmacopoeia Commission (2020). 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