{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T12:17:19Z","timestamp":1780575439332,"version":"3.54.1"},"reference-count":28,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2024,9,29]],"date-time":"2024-09-29T00:00:00Z","timestamp":1727568000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The increasing presence of Anisakis spp. in fish is having significant implications for public health due to a rise in cases of anisakiasis. Given this situation, there is a critical need to develop new strategies to fight this parasite. Satureja montana L., commonly known as savory, is a plant recognized in folk medicine for its therapeutic activity, such as being antispasmodic and digestive, among other properties. The aim of this study was to assess the nematicide activity against A. simplex larvae of the essential oil from two varieties of S. montana (subsp. montana (SMM) and variegata (SMV)). The essential oils were obtained via hydro-distillation of the flowering aerial parts. In vitro assays demonstrated the complete inactivation of anisakis larvae after 24 h when exposed to both essential oils, along with a significant reduction in their penetration capacity. Moreover, both essential oils showed an inhibitory effect on acetylcholinesterase (AChE). No differences between the subspecies were observed in any of the assays. Hence, the nematicidal activity of essential oils could be attributed to their capacity to inhibit AChE. These findings suggest the potential of S. montana essential oil for therapeutic and food industry applications.<\/jats:p>","DOI":"10.3390\/molecules29194640","type":"journal-article","created":{"date-parts":[[2024,9,30]],"date-time":"2024-09-30T06:39:35Z","timestamp":1727678375000},"page":"4640","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Essential Oils of Two Subspecies of Satureja montana L. against Gastrointestinal Parasite Anisakis simplex and Acetylcholinesterase Inhibition"],"prefix":"10.3390","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7972-7119","authenticated-orcid":false,"given":"Francisco","family":"Les","sequence":"first","affiliation":[{"name":"Department of Pharmacy, Faculty of Health Sciences, Universidad San Jorge, 50830 Villanueva de G\u00e1llego, Spain"},{"name":"Instituto Agroalimentario de Arag\u00f3n-IA2, CITA-Universidad de Zaragoza, 50013 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronica","family":"Galiffa","sequence":"additional","affiliation":[{"name":"Chemistry Interdiscplinary Project (ChIP) Research Center, School of Pharmacy, University of Camerino, Via Madonna delle Carceri 9\/B, 62032 Camerino, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9810-5497","authenticated-orcid":false,"given":"Guillermo","family":"C\u00e1sedas","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Health Sciences, Universidad San Jorge, 50830 Villanueva de G\u00e1llego, Spain"},{"name":"Instituto Agroalimentario de Arag\u00f3n-IA2, CITA-Universidad de Zaragoza, 50013 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7523-0415","authenticated-orcid":false,"given":"Cristina","family":"Moliner","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Health Sciences, Universidad San Jorge, 50830 Villanueva de G\u00e1llego, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1375-4744","authenticated-orcid":false,"given":"Filippo","family":"Maggi","sequence":"additional","affiliation":[{"name":"Chemistry Interdiscplinary Project (ChIP) Research Center, School of Pharmacy, University of Camerino, Via Madonna delle Carceri 9\/B, 62032 Camerino, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6969-1055","authenticated-orcid":false,"given":"V\u00edctor","family":"L\u00f3pez","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Health Sciences, Universidad San Jorge, 50830 Villanueva de G\u00e1llego, Spain"},{"name":"Instituto Agroalimentario de Arag\u00f3n-IA2, CITA-Universidad de Zaragoza, 50013 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3982-2282","authenticated-orcid":false,"given":"Carlota","family":"G\u00f3mez-Rinc\u00f3n","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Health Sciences, Universidad San Jorge, 50830 Villanueva de G\u00e1llego, Spain"},{"name":"Instituto Agroalimentario de Arag\u00f3n-IA2, CITA-Universidad de Zaragoza, 50013 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1002\/cbdv.201400112","article-title":"Composition of Leaf N-Alkanes in Three Satureja montana L. Subspecies from the Balkan Peninsula: Ecological and Taxonomic Aspects","volume":"12","author":"Matevski","year":"2015","journal-title":"Chem. Biodivers."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3143","DOI":"10.1080\/14786419.2018.1516661","article-title":"Comparison of Chemical Composition and Antioxidant Activities of Two Winter Savory Subspecies (Satureja montana Subsp. Variegata and Satureja montana Subsp. Montana) Cultivated in Northern Italy","volume":"33","author":"Caprioli","year":"2019","journal-title":"Nat. Prod. Res."},{"key":"ref_3","unstructured":"Olmstead, R. (2016). A Synoptical Classification of the Lamiales, University of Washington."},{"key":"ref_4","unstructured":"Aeschimann, D., Lauber, K., Moser, D., and Theurillat, J. (2004). Flora Alpina: Atlante Delle 4500 Piante Vascolari Delle Alpi, Zanichelli."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"375","DOI":"10.3109\/13880209.2015.1043560","article-title":"A Pharmacological and Phytochemical Overview on Satureja","volume":"54","author":"Tepe","year":"2016","journal-title":"Pharm. Biol."},{"key":"ref_6","first-page":"1035","article-title":"Chemical Composition, Olfactory Evaluation and Antioxidant Effects of the Essential Oil of Satureja montana L.","volume":"3","author":"Stoilova","year":"2008","journal-title":"Nat. Prod. Commun."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1623","DOI":"10.2174\/1389450116666150202161926","article-title":"Antimicrobial and Antioxidant Properties of Satureja montana L. and S. subspicata Vis. (Lamiaceae)","volume":"16","author":"Kremer","year":"2015","journal-title":"Curr. Drug Targets"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Said-Al Ahl, H.A.H., Ka\u010d\u00e1niova, M., Mahmoud, A.A., Hikal, W.M., \u010cmikov\u00e1, N., Szczepanek, M., B\u0142aszczyk, K., Al-Balawi, S.M., Bianchi, A., and Smaoui, S. (2024). Phytochemical Characterization and Biological Activities of Essential Oil from Satureja montana L., a Medicinal Plant Grown under the Influence of Fertilization and Planting Dates. Biology, 13.","DOI":"10.3390\/biology13050328"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"112378","DOI":"10.1016\/j.fct.2021.112378","article-title":"FEMA GRAS Assessment of Natural Flavor Complexes: Origanum Oil, Thyme Oil and Related Phenol Derivative-Containing Flavoring Ingredients","volume":"155","author":"Cohen","year":"2021","journal-title":"Food Chem. Toxicol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Cavallero, S., Bellini, I., Pizzarelli, A., and D\u2019amelio, S. (2022). What Do In Vitro and In Vivo Models Tell Us about Anisakiasis? New Tools Still to Be Explored. Pathogens, 11.","DOI":"10.3390\/pathogens11030285"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"578","DOI":"10.20344\/amp.15908","article-title":"Anisakis Allergy: Raising Awareness","volume":"35","author":"Rama","year":"2021","journal-title":"Acta Med. Port."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1146\/annurev-food-022811-101241","article-title":"Novel Natural Food Antimicrobials","volume":"3","author":"Juneja","year":"2012","journal-title":"Annu. Rev. Food Sci. Technol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1016\/j.apjtm.2015.07.017","article-title":"Natural Products: Perspectives in the Pharmacological Treatment of Gastrointestinal Anisakiasis","volume":"8","author":"Valero","year":"2015","journal-title":"Asian Pac. J. Trop. Med."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"471","DOI":"10.2174\/138945012799499703","article-title":"Novel and Viable Acetylcholinesterase Target Site for Developing Effective and Environmentally Safe Insecticides","volume":"13","author":"Pang","year":"2012","journal-title":"Curr. Drug Targets"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.1080\/10942912.2011.578319","article-title":"Antioxidant and Antihemolytic Activities of Ethanolic Extract of Flowers, Leaves, and Stems of Hyssopus officinalis L. Var","volume":"16","author":"Alinezhad","year":"2013","journal-title":"angustifolius. Int. J. Food Prop."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Ebadollahi, A., Jalali Sendi, J., Ziaee, M., and Krutmuang, P. (2021). Acaricidal, Insecticidal, and Nematicidal Efficiency of Essential Oils Isolated from the Satureja Genus. Int. J. Environ. Res. Public Health, 18.","DOI":"10.3390\/ijerph18116050"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"7452","DOI":"10.1021\/acs.jafc.6b03279","article-title":"Bioactivity of Ruta Graveolens and Satureja Montana Essential Oils on Solanum Tuberosum Hairy Roots and Solanum Tuberosum Hairy Roots with Meloidogyne Chitwoodi Co-Cultures","volume":"64","author":"Faria","year":"2016","journal-title":"J. Agric. Food Chem."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"12312","DOI":"10.3390\/molecules171012312","article-title":"Bioassays against Pinewood Nematode: Assessment of a Suitable Dilution Agent and Screening for Bioactive Essential Oils","volume":"17","author":"Barbosa","year":"2012","journal-title":"Molecules"},{"key":"ref_19","first-page":"8","article-title":"Nematicidal Activity of Essential Oils and Volatiles Derived from Portuguese Aromatic Flora against the Pinewood Nematode, Bursaphelenchus Xylophilus","volume":"42","author":"Barbosa","year":"2010","journal-title":"J. Nematol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"\u0160trbac, F., Bosco, A., Maurelli, M.P., Ratajac, R., Stojanovi\u0107, D., Simin, N., Or\u010di\u0107, D., Pu\u0161i\u0107, I., Krnjaji\u0107, S., and Sotiraki, S. (2022). Anthelmintic Properties of Essential Oils to Control Gastrointestinal Nematodes in Sheep-In Vitro and In Vivo Studies. Vet. Sci., 9.","DOI":"10.3390\/vetsci9020093"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"11557","DOI":"10.1021\/jf901786p","article-title":"Phytochemical Profile and Anticholinesterase and Antimicrobial Activities of Supercritical versus Conventional Extracts of Satureja Montana","volume":"57","author":"Silva","year":"2009","journal-title":"J. Agric. Food Chem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1002\/ptr.2063","article-title":"In Vitro Acetylcholinesterase Inhibitory Properties of Thymol, Carvacrol and Their Derivatives Thymoquinone and Thymohydroquinone","volume":"21","author":"Jukic","year":"2007","journal-title":"Phytother. Res."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"L\u00f3pez, V., Pavela, R., G\u00f3mez-Rinc\u00f3n, C., Les, F., Bartolucci, F., Galiffa, V., Petrelli, R., Cappellacci, L., Maggi, F., and Canale, A. (2019). Efficacy of Origanum Syriacum Essential Oil against the Mosquito Vector Culex Quinquefasciatus and the Gastrointestinal Parasite Anisakis Simplex, with Insights on Acetylcholinesterase Inhibition. Molecules, 24.","DOI":"10.3390\/molecules24142563"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1089\/fpd.2011.1076","article-title":"Anisakis Simplex Sensu Stricto and Anisakis Pegreffii: Biological Characteristics and Pathogenetic Potential in Human Anisakiasis","volume":"9","author":"Arizono","year":"2012","journal-title":"Foodborne Pathog. Dis."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2015\/983656","article-title":"Pathogenic Potential of Two Sibling Species, Anisakis Simplex (s.s.) and Anisakis Pegreffii (Nematoda: Anisakidae): In Vitro and in Vivo Studies","volume":"2015","author":"Jeon","year":"2015","journal-title":"Biomed. Res. Int."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/0006-2952(61)90145-9","article-title":"A New and Rapid Colorimetric Determination of Acetylcholinesterase Activity","volume":"7","author":"Ellman","year":"1961","journal-title":"Biochem. Pharmacol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2049","DOI":"10.1039\/C5FO00426H","article-title":"Bioactive Properties of Commercialised Pomegranate (Punica granatum) Juice: Antioxidant, Antiproliferative and Enzyme Inhibiting Activities","volume":"6","author":"Les","year":"2015","journal-title":"Food Funct."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/S0014-5793(99)01637-3","article-title":"Structure of Acetylcholinesterase Complexed with (-)-Galanthamine at 2.3 A Resolution","volume":"463","author":"Greenblatt","year":"1999","journal-title":"FEBS Lett."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/29\/19\/4640\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:07:04Z","timestamp":1760112424000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/29\/19\/4640"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,29]]},"references-count":28,"journal-issue":{"issue":"19","published-online":{"date-parts":[[2024,10]]}},"alternative-id":["molecules29194640"],"URL":"https:\/\/doi.org\/10.3390\/molecules29194640","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,29]]}}}