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Polarization modulation infrared reflection adsorption spectroscopy (PM-IRRAS) confirmed the presence of Fn on the surfaces. The Fn monolayer attached to the SAM was found to be stable under fluid shear stress. Deconvolution of the Fn amide I band indicated that the secondary structure of Fn changes significantly upon immobilization to the SAM-functionalized metal substrate. Scanning electron microscopy and energy dispersive X-ray analysis revealed that the spacing between Fn molecules on a modified commercial stent surface is approximately 66 nm, which has been reported to be the most appropriate spacing for cell\/surface interactions.<\/jats:p>","DOI":"10.3390\/molecules29204927","type":"journal-article","created":{"date-parts":[[2024,10,18]],"date-time":"2024-10-18T06:46:52Z","timestamp":1729234012000},"page":"4927","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Modification of 316L Stainless Steel, Nickel Titanium, and Cobalt Chromium Surfaces by Irreversible Immobilization of Fibronectin: Towards Improving the Coronary Stent Biocompatibility"],"prefix":"10.3390","volume":"29","author":[{"given":"Hesam","family":"Dadafarin","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering, McGill University, 3610 University St., Montreal, QC H3A 0C5, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evgeny","family":"Konkov","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, McGill University, 3610 University St., Montreal, QC H3A 0C5, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hojatollah","family":"Vali","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology, McGill University, 3640 University St., Montreal, QC H3A 0C7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irshad","family":"Ali","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Faculty of Mechanical, Chemical and Industrial Engineering, University of Engineering & Technology, Jamrud Road, Peshawar 25000, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9932-4272","authenticated-orcid":false,"given":"Sasha","family":"Omanovic","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, McGill University, 3610 University St., Montreal, QC H3A 0C5, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,10,18]]},"reference":[{"key":"ref_1","first-page":"1","article-title":"A Review on Surface Coatings on 316 L Stainless Steel to Improve Biomedical Properties","volume":"4","author":"GouthamMurari","year":"2015","journal-title":"Int. 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