{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T02:33:47Z","timestamp":1778726027819,"version":"3.51.4"},"reference-count":67,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2024,10,20]],"date-time":"2024-10-20T00:00:00Z","timestamp":1729382400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Snow mountain garlic is traditionally eaten by Himalayan locals for its medicinal properties. Although different species of the genus Allium are known to have other biological effects, such as antiplatelet and antithrombotic activities, little is known about the anticoagulant effect of Snow mountain garlic, a member of the genus Allium. Therefore, the present study examined the in vitro anticoagulant effect of the aqueous extract, the lyophilized aqueous extract, and the isoflavone extract from the lyophilized aqueous extract of Snow mountain garlic in samples from 50 human blood donors. Compared to the control, concentrations of 25, 12.5, and 6.25 mg\/100 \u00b5L lengthened the clotting times of prothrombin, and concentrations of 25 and 12.5 mg\/100 \u00b5L lengthened the activated partial thromboplastin time (p \u02c2 0.05). The isoflavone extract from the lyophilized aqueous extract containing isoflavones, organosulfur compounds, a polyphenol, and a steroid glycoside showed a significant effect (p \u02c2 0.05) on the prothrombin time and the activated partial thromboplastin time at a dose of 20 \u00b5L (volume) compared to the control. The results regarding the use of Snow mountain garlic as a preventive measure and aid in treating thromboembolic disease are promising.<\/jats:p>","DOI":"10.3390\/molecules29204958","type":"journal-article","created":{"date-parts":[[2024,10,21]],"date-time":"2024-10-21T09:58:24Z","timestamp":1729504704000},"page":"4958","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Anticoagulant Effect of Snow mountain garlic: In Vitro Evaluation of Aqueous Extract"],"prefix":"10.3390","volume":"29","author":[{"given":"Isabel","family":"Clark-Montoya","sequence":"first","affiliation":[{"name":"Faculty of Chemical Sciences, Autonomous University of San Luis Potosi, Dr. Manuel Nava Mart\u00ednez Avenue #6, University Zone, San Luis Potosi 78210, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6118-6393","authenticated-orcid":false,"given":"Yolanda","family":"Ter\u00e1n-Figueroa","sequence":"additional","affiliation":[{"name":"Faculty of Nursing and Nutrition, Autonomous University of San Luis Potosi, Ni\u00f1o Artillero Avenue #130, University Zone, San Luis Potosi 78240, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5291-2197","authenticated-orcid":false,"given":"Denisse","family":"de Loera","sequence":"additional","affiliation":[{"name":"Faculty of Chemical Sciences, Autonomous University of San Luis Potosi, Dr. Manuel Nava Mart\u00ednez Avenue #6, University Zone, San Luis Potosi 78210, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dar\u00edo","family":"Gayt\u00e1n-Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Faculty of Nursing and Nutrition, Autonomous University of San Luis Potosi, Ni\u00f1o Artillero Avenue #130, University Zone, San Luis Potosi 78240, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge Alejandro","family":"Alegr\u00eda-Torres","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University of Guanajuato, Noria Alta s\/n, Building I, Guanajuato 36050, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosa del Carmen","family":"Mil\u00e1n-Segovia","sequence":"additional","affiliation":[{"name":"Faculty of Chemical Sciences, Autonomous University of San Luis Potosi, Dr. Manuel Nava Mart\u00ednez Avenue #6, University Zone, San Luis Potosi 78210, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,10,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e583","DOI":"10.1016\/S2352-3026(20)30211-8","article-title":"Mortality risk associated with venous thromboembolism: A systematic review and Bayesian meta-analysis","volume":"7","author":"Klemen","year":"2020","journal-title":"Lancet Haematol."},{"key":"ref_2","unstructured":"Gobierno de M\u00e9xico, and Direcci\u00f3n General de Epidemiologia, Secretar\u00eda de Salud (2024). Bolet\u00edn Epidemiol\u00f3gico. Sistema Nacional de Vigilancia Epidemiol\u00f3gica. Sistema \u00danico de Informaci\u00f3n. Semana Epidemiol\u00f3gica, 2, 41. Available online: https:\/\/www.gob.mx\/cms\/uploads\/attachment\/file\/883562\/Bole_calendario.pdf."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.blre.2017.02.001","article-title":"Old and new oral anticoagulants: Food, herbal medicines, and drug interactions","volume":"31","author":"Frigerio","year":"2017","journal-title":"Revew. Blood Rev."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1111\/jth.15126","article-title":"New anticoagulants: Moving beyond the direct oral anticoagulants","volume":"19","author":"Fredenburgh","year":"2021","journal-title":"J. Thromb. Haemost."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"100615","DOI":"10.1016\/j.blre.2019.100615","article-title":"From multi-target anticoagulants to DOACs, and intrinsic coagulation factor inhibitors","volume":"39","author":"Lin","year":"2020","journal-title":"Blood Rev."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2987","DOI":"10.1016\/j.jacc.2021.04.061","article-title":"Meta-analysis of reversal agents for severe bleeding associated with direct oral anticoagulants","volume":"77","author":"Alcubilla","year":"2021","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_7","first-page":"69","article-title":"Development of new anticoagulant in 2023: Prime time for anti-factor XI and XIa inhibitors","volume":"48","author":"Bentounes","year":"2023","journal-title":"J. Med. Vasc."},{"key":"ref_8","unstructured":"Samuelsson, G. (1999). Drugs of Natural Origin: A Textbook of Pharmacognosy, Swedish Pharmaceutical Press. [4th ed.]."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"115939","DOI":"10.1016\/j.jep.2022.115939","article-title":"Validation of traditional claims of anti-arthritic efficacy of trans-Himalayan snow mountain garlic (Allium ampeloprasum L.) extract using adjuvant-induced arthritis rat model: A comparative evaluation with normal garlic (Allium sativum L.) and dexamethasone","volume":"303","author":"Kaur","year":"2023","journal-title":"J. Ethnopharmacol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Batiha, G.-S., Beshbishy, A.M., Adeyemi, O.S., Nadwa, E.H., Rashwan, E.M., Alkazmi, L.M., Elkelish, A.A., and Igarashi, I. (2020). Phytochemical screening and antiprotozoal effects of the methanolic Berberis vulgaris and acetonic Rhus coriaria extracts. Molecules, 25.","DOI":"10.3390\/molecules25030550"},{"key":"ref_11","first-page":"9679739","article-title":"In vitro inhibitory and proliferative cellular effects of different extracts of Struthanthus quercicola: A preliminary study","volume":"2022","year":"2022","journal-title":"Evid. Based Complement. Altern. Med."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"114577","DOI":"10.1016\/j.jep.2021.114577","article-title":"Metabolite profiling, anti-inflammatory, analgesic potentials of edible herb Colocasia gigantea and molecular docking study against COX-II enzyme","volume":"281","author":"Zilani","year":"2021","journal-title":"J. Ethnopharmacol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1007\/s43450-022-00263-9","article-title":"Antidiabetic Activity and Hepatotoxic Effect of Aqueous Extracts of Struthanthus quercicola","volume":"32","year":"2022","journal-title":"Rev. Bras. Farmacogn."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1007\/978-1-4939-3332-7_23","article-title":"In vitro and cryopreservation techniques for conservation of Snow Mountain Garlic","volume":"1391","author":"Mahajan","year":"2016","journal-title":"Methods Mol. Biol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.ygeno.2019.07.014","article-title":"Transcriptome analysis of Snow Mountain Garlic for unraveling the organosulfur metabolic pathway","volume":"112","author":"Mehra","year":"2020","journal-title":"Genomics"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1007\/s10722-012-9819-5","article-title":"Diversity in Allium ampeloprasum: From small and wild to large and cultivated","volume":"60","author":"Guenaoui","year":"2013","journal-title":"Genet. Resour. Crop. Evol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/j.scienta.2007.06.021","article-title":"Is single bulb producing garlic Allium sativum or Allium ampeloprasum?","volume":"114","author":"Figliuolo","year":"2007","journal-title":"Sci. Hortic."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Ter\u00e1n-Figueroa, Y., de Loera, D., Toxqui-Ter\u00e1n, A., Montero-Mor\u00e1n, G., and Saavedra-Leos, M.Z. (2022). Bromatological Analysis and Characterization of Phenolics in Snow Mountain Garlic. Molecules, 27.","DOI":"10.3390\/molecules27123712"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1308","DOI":"10.1111\/jam.15537","article-title":"A comparative study of in-vitro and in-silico anti-candidal activity and GC\u2013MS profiles of snow mountain garlic vs. normal garlic","volume":"133","author":"Kaur","year":"2022","journal-title":"J. Appl. Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"736s","DOI":"10.1093\/jn\/136.3.736S","article-title":"Garlic and cardiovascular disease: A critical review","volume":"136","author":"Rahman","year":"2006","journal-title":"J. Nutr."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Liu, M., Wang, G., Xu, R., Shen, C., Ni, H., and Lai, R. (2021). Soy isoflavones inhibit both GPIb-IX signaling and \u03b1IIb\u03b23 outside-in signaling via 14-3-3\u03b6 in platelet. Molecules, 26.","DOI":"10.3390\/molecules26164911"},{"key":"ref_22","first-page":"115","article-title":"Los productos herbolarios, la coagulaci\u00f3n sangu\u00ednea y la cirug\u00eda otorrinolaringol\u00f3gica","volume":"62","year":"2017","journal-title":"An. De Otorrinolaringol. Mex."},{"key":"ref_23","unstructured":"Kour, J., and Nayik, G.A. (2022). Genistein and daidzein. Nutraceuticals and Health Care, Academic Press. Chapter 19."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1079\/BJN2003820","article-title":"Effect of genistein and daidzein on platelet aggregation and monocyte and endothelial function","volume":"89","author":"Gottstein","year":"2003","journal-title":"Br. J. Nutr."},{"key":"ref_25","first-page":"1271","article-title":"Isoflavonas de soya y evidencias sobre la protecci\u00f3n cardiovascular","volume":"29","year":"2014","journal-title":"Nutr. Hosp."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"184","DOI":"10.3390\/ddc3010011","article-title":"Plant-derived natural products: A source for drug discovery and development","volume":"3","author":"Chaachouay","year":"2024","journal-title":"Drugs Drug Candidates"},{"key":"ref_27","unstructured":"World Health Organization (2024, June 24). WHO Strategy on Traditional Medicine 2014\u20132023. WHO Editions. Available online: https:\/\/iris.who.int\/bitstream\/handle\/10665\/95008\/9789243506098_spa.pdf."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"643269","DOI":"10.1093\/ecam\/nen070","article-title":"N-acetylcysteine an Allium plant compound improves high-sucrose diet-induced obesity and related effects","volume":"2011","author":"Souza","year":"2011","journal-title":"Evid. Based Complement. Altern. Med."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1280","DOI":"10.1021\/jf062587s","article-title":"Effect of cooking on garlic (Allium sativum L.) antiplatelet activity and thiosulfinates content","volume":"55","author":"Cavagnaro","year":"2007","journal-title":"J. Agric. Food Chem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/S0952-3278(96)90014-9","article-title":"An evaluation of garlic and onion as antithrombotic agents","volume":"54","author":"Bordia","year":"1996","journal-title":"Prostaglandins Leukot. Essent. Fat. Acids"},{"key":"ref_31","first-page":"86","article-title":"Protective effect of garlic oil on the changes produced by 3 weeks of fatty diet on serum cholesterol, serum triglycerides, fibrinolytic activity, and platelet adhesiveness in man","volume":"34","author":"Bordia","year":"1982","journal-title":"Indian Heart J."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1007\/s12291-020-00901-w","article-title":"Analysis of intrinsic and extrinsic coagulation pathway factors in OCP treated PCOS women","volume":"36","author":"Manzoor","year":"2021","journal-title":"Indian J. Clin. Biochem."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1016\/0021-9150(78)90129-6","article-title":"Effect of garlic on human platelet aggregation in vitro","volume":"30","author":"Bordia","year":"1978","journal-title":"Atherosclerosis"},{"key":"ref_34","first-page":"301","article-title":"Antihaemostatic effect of the combination of Allium sativum L. ethanol extract and warfarin in Wistar rats","volume":"20","author":"Musubika","year":"2015","journal-title":"Rev. Cuba. De Plantas Med."},{"key":"ref_35","first-page":"386","article-title":"Pharmacological effect of Allium sativum on coagulation, blood pressure, diabetic nephropathy, neurological disorders, spermatogenesis, antibacterial effects","volume":"4","author":"Abbas","year":"2019","journal-title":"Agric. Food"},{"key":"ref_36","first-page":"1256","article-title":"In vitro anticoagulant activity of Allium sativum plant extract","volume":"6","author":"Vaijayanthimala","year":"2017","journal-title":"WJPPS"},{"key":"ref_37","first-page":"1485","article-title":"Short-term impact of aged garlic extract on endothelial function in diabetes: A randomized, double-blind, placebo-controlled trial","volume":"19","author":"Hamal","year":"2019","journal-title":"Exp. Ther. Med."},{"key":"ref_38","first-page":"120","article-title":"Effect of Allium sativum (Garlic) extract on blood coagulation and fibrinolysis","volume":"2","author":"Narsimha","year":"2017","journal-title":"Adv. Pharmacol. Clin. Trials"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/S0952-3278(98)90034-5","article-title":"Effect of garlic (Allium sativum) on blood lipids, blood sugar, fibrinogen, and fibrinolytic activity in patients with coronary artery disease","volume":"58","author":"Bordia","year":"1998","journal-title":"Prostaglandins Leukot. Essent. Fat. Acids"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1271\/bbb.60380","article-title":"Antithrombotic effects of odorless garlic powder both in vitro and in vivo","volume":"71","author":"Fukao","year":"2007","journal-title":"J. Biosci. Biotechnol. Biochem."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1016\/j.fct.2006.10.005","article-title":"Effect of diallyl trisulfide-rich garlic oil on blood coagulation and plasma activity of anticoagulation factors in rats","volume":"45","author":"Chan","year":"2007","journal-title":"Food Chem. Toxicol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.cbi.2016.07.022","article-title":"Popular naturally occurring antioxidants as potential anticoagulant drugs","volume":"257","author":"Bijak","year":"2016","journal-title":"Chem. Biol. Interact."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"7035","DOI":"10.2174\/0929867325666180417170218","article-title":"Flavonoids and Platelet-Derived Thrombotic Disorders","volume":"26","author":"Lopez","year":"2019","journal-title":"Curr. Med. Chem."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1689","DOI":"10.1080\/10942912.2016.1220393","article-title":"Natural polyphenols: An overview","volume":"20","author":"Abbas","year":"2017","journal-title":"Int. J. Food Prop."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"151","DOI":"10.12688\/f1000research.130105.1","article-title":"Determination of pharmacological activity of bioactives in Allium sativum using computational analysis [version 1; peer review: 2 approved with reservations]","volume":"12","author":"Ouma","year":"2023","journal-title":"F1000Research"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"972","DOI":"10.1124\/jpet.107.125690","article-title":"The inducible nitric-oxide synthase modulates endothelin-1-dependent release of prostacyclin and inhibition of platelet aggregation ex vivo in the mouse","volume":"323","author":"Carrier","year":"2007","journal-title":"Pharmacol. Exp. Ther."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"100323","DOI":"10.1016\/j.prmcm.2023.100323","article-title":"Mechanisms of action by sulphur compounds in Allium sativum. A review","volume":"9","year":"2023","journal-title":"Pharmacol. Res. Mod. Chin. Med."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"5731","DOI":"10.1021\/jf0004412","article-title":"Differential inhibition of human platelet aggregation by selected Allium thiosulfinates","volume":"48","author":"Briggs","year":"2000","journal-title":"J. Agric. Food Chem."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Hong, H.J., Nam, G.S., and Nam, K.S. (2023). Daidzein inhibits human platelet activation by downregulating thromboxane A2 production and granule release, regardless of COX-1 activity. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms241511985"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.phymed.2017.08.023","article-title":"The isoflavonoid tectorigenin has better antiplatelet potential than acetylsalicylic acid","volume":"35","year":"2017","journal-title":"Phytomedicine"},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Lee, B.K., Jee, H.J., and Jung, Y.S. (2021). A\u03b21-40-induced platelet adhesion is ameliorated by rosmarinic acid through inhibition of NADPH Oxidase\/PKC-\u03b4\/Integrin \u03b1IIb\u03b23 signaling. Antioxidants, 10.","DOI":"10.3390\/antiox10111671"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1159\/000337380","article-title":"Allium ursinum L: Bioassay-guided isolation and identification of a galactolipid and a phytosterol exerting antiaggregatory effects","volume":"89","author":"Sabha","year":"2012","journal-title":"Pharmacology"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"128011","DOI":"10.1016\/j.foodchem.2020.128011","article-title":"Comparative phytochemical profile of the elephant garlic (Allium ampeloprasum var. holmense) and the common garlic (Allium sativum) from the Val di Chiana area (Tuscany, Italy) before and after in vitro gastrointestinal digestion","volume":"338","author":"Ceccanti","year":"2021","journal-title":"Food Chem."},{"key":"ref_54","unstructured":"Genetic Alliance, and The New York-Mid-Atlantic Consortium for Genetic and Newborn Screening Services (2024, June 17). Understanding Genetics: A Guide for Patients and Medical Professionals in the New York and Mid-Atlantic Region, Available online: https:\/\/www.ncbi.nlm.nih.gov\/books\/NBK132199\/."},{"key":"ref_55","unstructured":"United States of Mexico (2014). Regulations of the General Health Law on Research for Health. Off. J. Fed., 5\u201331. Available online: https:\/\/www.diputados.gob.mx\/LeyesBiblio\/regley\/Reg_LGS_MIS.pdf."},{"key":"ref_56","unstructured":"(2024, June 18). World Medical Assembly. Helsinki Declaration. Ethical Principles for Medical Research in Humans. 18. World Medical Assembly. Available online: https:\/\/www.wma.net\/es\/policies-post\/declaracion-de-helsinki-de-la-amm-principios-eticos-para-las-investigaciones-medicas-en-seres-humanos\/."},{"key":"ref_57","unstructured":"United States of Mexico (2003). Ministry of Environment and Natural Resources the Management of Biological Waste Will Be Carried out as Established by the Official Mexican Standard NOM-087-ECOL-SSA1-2002, Environmental Protection-Environmental Health-Biological-Infectious Hazardous Waste-Classification and Management Specifications. Off. J. Fed., 10\u201323. Available online: https:\/\/www.cndh.org.mx\/sites\/default\/files\/doc\/Programas\/VIH\/Leyes%20y%20normas%20y%20reglamentos\/Norma%20Oficial%20Mexicana\/NOM-087-SEMARNAT-SSA1-2002%20Proteccion%20ambiental-salud.pdf."},{"key":"ref_58","unstructured":"Bruneton, J. (1991). Elementos de Fitoqu\u00edmica y de Farmacognosia, Acriba."},{"key":"ref_59","unstructured":"Dom\u00ednguez, X.A. (1985). M\u00e9todos de Investigaci\u00f3n Fitoqu\u00edmica, Limusa."},{"key":"ref_60","unstructured":"Trease, G.E., and Evans, W.C. (1987). Tratado de Farmacognosia, Interamericana."},{"key":"ref_61","first-page":"21","article-title":"Preliminary phytochemical analysis of leaf extracts Ofthuja orientalis (L.) Endl","volume":"2","author":"Trang","year":"2015","journal-title":"Int. J. Res. Sci. Mang."},{"key":"ref_62","unstructured":"S\u00e1nchez Cubides, Y., Santacruz Garc\u00eda, Y.A.C., T\u00e9llez Chac\u00f3n, N.A., Villa Jim\u00e9nez, D.Y., De Antonio Rinc\u00f3n, M.A., Mateus G\u00f3mez, A., Corzo Gualdr\u00f3n, A.M., Moreno Ospina, A.A., Cort\u00e9s Santana, M., and Mart\u00ednez C\u00e1rdenas, L.E. (2010). Fitoqu\u00edmica De Cinco Especies Del G\u00e9nero Baccharis (B. boyacensis Cuatr, B. lehmannii Klatt, B. macrantha Kunth, B. bogotensis Kunth, B. mutisiana Cuatrec) End\u00e9micas del Altiplano Cundiboyacense. [Bachelor Degree Thesis, Universidad Distrital Francisco Jos\u00e9 de Caldas, Facultad de Ciencias y Educaci\u00f3n]. Available online: http:\/\/hdl.handle.net\/11349\/910."},{"key":"ref_63","first-page":"30","article-title":"An\u00e1lisis fitoqu\u00edmico preliminar de Pachira quinata (Jacq.) W.S. Alverson, Bogot\u00e1, Colombia","volume":"11","year":"2017","journal-title":"Bolet\u00edn Semillas Ambient. Bogot\u00e1 Colomb."},{"key":"ref_64","unstructured":"AOAC (Association of Official Agricultural Chemists) (1990). Official Methods of Analysis 954.01, AOAC. [15th ed.]."},{"key":"ref_65","unstructured":"United States of Mexico, Ministry of Economy, Undersecretary of Competitiveness and Regulations (2017). General Directorate of Standards. Mexican Standard NMX-F-312-NORMEX-2016, Food-Determination of Reducing Sugars in Foods and Non-Alcoholic Beverages-Test Method. Off. J. Fed., Available online: https:\/\/dof.gob.mx\/nota_detalle.php?codigo=5495910&fecha=01\/09\/2017#gsc.tab=0."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1674","DOI":"10.1021\/jf00044a017","article-title":"Isoflavone composition of American and Japanese soybeans in Iowa: Effects of variety, crop year, and location","volume":"42","author":"Wang","year":"1994","journal-title":"J. Agric. Food Chem."},{"key":"ref_67","unstructured":"Sabha, D. (2011). Pharmaceutical and chemical analysis of the components carrying the antiplatelet activity of extracts from Allium ursinum and Allium sativum. [Doctoral Thesis, Medical Faculty the University of Leipzig]. Available online: https:\/\/katalog.slub-dresden.de\/en\/id\/0-682964964api."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/29\/20\/4958\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:16:59Z","timestamp":1760113019000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/29\/20\/4958"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,20]]},"references-count":67,"journal-issue":{"issue":"20","published-online":{"date-parts":[[2024,10]]}},"alternative-id":["molecules29204958"],"URL":"https:\/\/doi.org\/10.3390\/molecules29204958","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,20]]}}}