{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:19:59Z","timestamp":1792790399281,"version":"4.5.0"},"reference-count":50,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2024,10,22]],"date-time":"2024-10-22T00:00:00Z","timestamp":1729555200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Wound healing is a complex process that can be improved through advanced biomedical approaches. Incorporating nanopolymers and plant extracts into wound dressings offers a favorable strategy for promoting tissue repair. Nanopolymers provide a controlled environment for sustained drug release while also protecting the wound from external contaminants. When combined with bioactive compounds from plant extracts, which possess antioxidant, anti-inflammatory, and antimicrobial properties, this hybrid approach can accelerate healing, reduce infection, and improve tissue regeneration. Hence, in this study, we have synthesized alginate\/gelatin hydrogel blended with only nanosilver (Alg\/gel-Ag) and with nanosilver and plant extracts like aloe vera, curcumin, plantain peel extract, and Calendula flower petal extract (Alg\/gel-AgP). The synthesized hydrogels were characterized using different photophysical tools, and the cytotoxicity effect was studied using a fibroblast cell line (V79). The antibacterial effect of the hydrogels was also observed against E. coli and S. aureus, determining the MIC and MBC. The wound healing in vitro was also assessed using scratch assay which depicted a rapid wound closure for Alg\/gel-AgP compared to the untreated control and Alg\/gel-Ag. The combined effect between nanotechnology and natural extracts represents a novel and effective approach for enhancing the wound healing process.<\/jats:p>","DOI":"10.3390\/molecules29215004","type":"journal-article","created":{"date-parts":[[2024,10,23]],"date-time":"2024-10-23T04:28:43Z","timestamp":1729657723000},"page":"5004","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Enhancing Wound Healing with Nanohydrogel-Entrapped Plant Extracts and Nanosilver: An In Vitro Investigation"],"prefix":"10.3390","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-0985-8344","authenticated-orcid":false,"given":"Devadass","family":"Jessy Mercy","sequence":"first","affiliation":[{"name":"Medical Bionanotechnology, Faculty of Allied Health Sciences, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Chettinad Health City, Kelambakkam, Chennai 603103, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2659-5191","authenticated-orcid":false,"given":"Anbazhagan","family":"Thirumalai","sequence":"additional","affiliation":[{"name":"Medical Bionanotechnology, Faculty of Allied Health Sciences, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Chettinad Health City, Kelambakkam, Chennai 603103, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-3655-4689","authenticated-orcid":false,"given":"Saranya","family":"Udayakumar","sequence":"additional","affiliation":[{"name":"Medical Bionanotechnology, Faculty of Allied Health Sciences, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Chettinad Health City, Kelambakkam, Chennai 603103, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4441-7863","authenticated-orcid":false,"given":"Balasubramanian","family":"Deepika","sequence":"additional","affiliation":[{"name":"Medical Bionanotechnology, Faculty of Allied Health Sciences, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Chettinad Health City, Kelambakkam, Chennai 603103, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-7601-3832","authenticated-orcid":false,"given":"Gopalarethinam","family":"Janani","sequence":"additional","affiliation":[{"name":"Medical Bionanotechnology, Faculty of Allied Health Sciences, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Chettinad Health City, Kelambakkam, Chennai 603103, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0475-2544","authenticated-orcid":false,"given":"Agnishwar","family":"Girigoswami","sequence":"additional","affiliation":[{"name":"Medical Bionanotechnology, Faculty of Allied Health Sciences, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Chettinad Health City, Kelambakkam, Chennai 603103, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1554-5241","authenticated-orcid":false,"given":"Koyeli","family":"Girigoswami","sequence":"additional","affiliation":[{"name":"Medical Bionanotechnology, Faculty of Allied Health Sciences, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Chettinad Health City, Kelambakkam, Chennai 603103, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,10,22]]},"reference":[{"key":"ref_1","unstructured":"Barabadi, H., Saravanan, M., Mostafavi, E., and Vahidi, H. (2023). Chapter 2\u2014An overview of wound healing: Wound types and current therapeutics. Bioengineered Nanomaterials for Wound Healing and Infection Control, Woodhead Publishing."},{"key":"ref_2","first-page":"1","article-title":"Nanostructure-assisted wound dressing materials: A literature review","volume":"24","author":"Biswas","year":"2024","journal-title":"Macromol. Res."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"147","DOI":"10.33899\/iphr.2022.174809","article-title":"A Review in the View on Wounds","volume":"19","author":"Qasim","year":"2022","journal-title":"Iraqi J. Pharm."},{"key":"ref_4","first-page":"62","article-title":"A systematic review on wound healing","volume":"8","author":"Quraishi","year":"2023","journal-title":"Int. J. Indig. Herbs Drugs"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Sah, M.K., Kasoju, N., and Mano, J.F. (2022). Chapter 1\u2014Fundamentals of skin wound healing and repair: A brief review on cellular and molecular pathophysiologic basis of wound healing. Natural Polymers in Wound Healing and Repair, Elsevier.","DOI":"10.1016\/B978-0-323-90514-5.00017-1"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"128499","DOI":"10.1016\/j.ijbiomac.2023.128499","article-title":"Recent progress in polysaccharide and polypeptide based modern moisture-retentive wound dressings","volume":"256","author":"Kamaraj","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Sikka, M.P., Bargir, J.A., and Garg, S. (2024). Modern developments in burn wound dressing. Res. J. Text. Appar., ahead-of-print.","DOI":"10.1108\/RJTA-08-2023-0084"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1186\/s11671-024-04061-1","article-title":"Greener healing: Sustainable nanotechnology for advanced wound care","volume":"19","author":"Sharda","year":"2024","journal-title":"Discov. Nano"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1016\/j.jconrel.2021.07.017","article-title":"Nanomaterials applied in wound healing: Mechanisms, limitations and perspectives","volume":"337","author":"Wang","year":"2021","journal-title":"J. Control. Release"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Aderibigbe, B.A., and Buyana, B. (2018). Alginate in Wound Dressings. Pharmaceutics, 10.","DOI":"10.3390\/pharmaceutics10020042"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"4344","DOI":"10.1021\/acs.biomac.4c00406","article-title":"Remendable Cross-Linked Alginate\/Gelatin Hydrogels Incorporating Nanofibers for Wound Repair and Regeneration","volume":"25","author":"Hong","year":"2024","journal-title":"Biomacromolecules"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"123438","DOI":"10.1016\/j.ijbiomac.2023.123438","article-title":"Synthesis and evaluation of alginate, gelatin, and hyaluronic acid hybrid hydrogels for tissue engineering applications","volume":"233","author":"Serafin","year":"2023","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"50","DOI":"10.2174\/0124681873294822240517073406","article-title":"Nanomaterials in Wound Healing: Mechanisms, Applications, and Future Prospects","volume":"15","author":"Rani","year":"2024","journal-title":"Curr. Nanomed."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1007\/s40883-022-00265-y","article-title":"Plant Extract\u2013Derived Nanomaterials for Wound Healing: A Mini Review","volume":"9","author":"Bhoopathy","year":"2023","journal-title":"Regen. Eng. Transl. Med."},{"key":"ref_15","first-page":"212","article-title":"A review: Silver nanoparticles in wound healing","volume":"8","author":"Vyavahare","year":"2021","journal-title":"Eur. J. Pharm. Med. Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"3455","DOI":"10.1007\/s12668-024-01535-5","article-title":"Phytonanotherapeutic Applications of Plant Extract-Synthesized Silver Nanoparticles in Wound Healing\u2014A Prospective Overview","volume":"14","author":"Oselusi","year":"2024","journal-title":"BioNanoScience"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Diniz, F.R., Maia, R.C.A.P., de Andrade, L.R.M., Andrade, L.N., Vinicius Chaud, M., da Silva, C.F., Corr\u00eaa, C.B., de Albuquerque Junior, R.L.C., Pereira da Costa, L., and Shin, S.R. (2020). Silver Nanoparticles-Composing Alginate\/Gelatine Hydrogel Improves Wound Healing In Vivo. Nanomaterials, 10.","DOI":"10.3390\/nano10020390"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"6338","DOI":"10.1038\/s41598-020-63464-z","article-title":"Delayed application of silver nanoparticles reveals the role of early inflammation in burn wound healing","volume":"10","author":"Zhang","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"4186","DOI":"10.24294\/ti.v8.i2.4186","article-title":"A potential role of nanophytocompounds in diabetic foot ulcers","volume":"8","author":"Thapa","year":"2024","journal-title":"Trends Immunother."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1080\/07388551.2023.2193859","article-title":"Electrospun wound dressings with antibacterial function: A critical review of plant extract and essential oil incorporation","volume":"44","author":"Mouro","year":"2024","journal-title":"Crit. Rev. Biotechnol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1002\/jemt.24438","article-title":"Fabrication of wound dressings: Herbal extract-loaded nanoliposomes embedded in fungal chitosan\/polycaprolactone electrospun nanofibers for tissue regeneration","volume":"87","author":"Demirel","year":"2024","journal-title":"Microsc. Res. Tech."},{"key":"ref_22","first-page":"100199","article-title":"Calendula in modern medicine: Advancements in wound healing and drug delivery applications","volume":"12","author":"Ejiohuo","year":"2024","journal-title":"Eur. J. Med. Chem. Rep."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"e20837","DOI":"10.1590\/s2175-97902022e20837","article-title":"Aloe vera and wound healing: A brief review","volume":"58","author":"Massoud","year":"2023","journal-title":"Braz. J. Pharm. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Al-Musawi, S., Albukhaty, S., Al-Karagoly, H., Sulaiman, G.M., Alwahibi, M.S., Dewir, Y.H., Soliman, D.A., and Rizwana, H. (2020). Antibacterial activity of honey\/chitosan nanofibers loaded with capsaicin and gold nanoparticles for wound dressing. Molecules, 25.","DOI":"10.3390\/molecules25204770"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1111\/jop.12789","article-title":"Aloe vera in treatment of oral submucous fibrosis: A systematic review and meta-analysis","volume":"48","author":"Ashraf","year":"2019","journal-title":"J. Oral Pathol. Med."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1080\/24694193.2020.1727065","article-title":"Efficacy of Aloe-Vera Use for Prevention of Chemotherapy-Induced Oral Mucositis in Children with Acute Lymphoblastic Leukemia: A Randomized Controlled Clinical Trial","volume":"44","author":"Alkhouli","year":"2020","journal-title":"Compr. Child Adolesc. Nurs."},{"key":"ref_27","first-page":"216","article-title":"Anti-Inflammatory Activity of the Peel Extract of Ambon Bananas (Musa paradisiaca L.) Examined with the Human Red Blood Cell Membrane Stabilization Method","volume":"2022","author":"Khairinisa","year":"2022","journal-title":"KnE Med."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Oyeyinka, B.O., and Afolayan, A.J. (2020). Potentials of Musa Species Fruits against Oxidative Stress-Induced and Diet-Linked Chronic Diseases: In Vitro and In Vivo Implications of Micronutritional Factors and Dietary Secondary Metabolite Compounds. Molecules, 25.","DOI":"10.3390\/molecules25215036"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"53","DOI":"10.5937\/scriptamed54-42580","article-title":"The Gastroprotective Role of Yellow Kepok Banana (Musa x paradisiaca L. var. Kepok) Peel Extract and Influence on Markers of Oxidative Stress: Malondialdehyde and Nitric Oxide","volume":"54","author":"Samiasih","year":"2023","journal-title":"Scr. Medica"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"4503","DOI":"10.2147\/DDDT.S327378","article-title":"Anti-Inflammatory Effects of Curcumin in the Inflammatory Diseases: Status, Limitations and Countermeasures","volume":"15","author":"Peng","year":"2021","journal-title":"Drug Des. Dev. Ther."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"4293","DOI":"10.1021\/acsabm.4c00111","article-title":"Stimuli-Responsive Codelivery System-Embedded Polymeric Nanofibers with Synergistic Effects of Growth Factors and Low-Intensity Pulsed Ultrasound to Enhance Osteogenesis Properties","volume":"7","author":"Malekmohammadi","year":"2024","journal-title":"ACS Appl. Bio Mater."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"4405","DOI":"10.1021\/acsbiomaterials.9b00237","article-title":"Design and Synthesis of Gatekeeper Coated Dendritic Silica\/Titania Mesoporous Nanoparticles with Sustained and Controlled Drug Release Properties for Targeted Synergetic Chemo-Sonodynamic Therapy","volume":"5","author":"Malekmohammadi","year":"2019","journal-title":"ACS Biomater. Sci. Eng."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"134814","DOI":"10.1016\/j.ijbiomac.2024.134814","article-title":"Exploring the amyloid degradation potential of nanoformulated carrageenan-bridging in vitro and in vivo perspectives","volume":"279","author":"Udayakumar","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1","DOI":"10.47545\/etrj.2020.5.1.054","article-title":"Comparative characterization of plantain peel, pawpaw peel and watermelon rind using FTIR","volume":"5","author":"Akinyemi","year":"2020","journal-title":"J. Eng. Technol. Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"102910","DOI":"10.1016\/j.bcab.2023.102910","article-title":"A systemic review on Aloe vera derived natural biomaterials for wound healing applications","volume":"54","author":"Rajesh","year":"2023","journal-title":"Biocatal. Agric. Biotechnol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"327","DOI":"10.51470\/PLANTARCHIVES.2023.v23.no2.056","article-title":"Phytochemistry, pharmacology and toxicity of Aloe vera: A versatile plant with extensive therapeutic potential","volume":"23","author":"Pradhan","year":"2023","journal-title":"Plant Arch."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"130","DOI":"10.21276\/apjhs.2022.9.2.26","article-title":"Antioxidant activity in the leaves and petals of Calendula officinalis Linn","volume":"9","author":"Pandey","year":"2022","journal-title":"Asian Pac. J. Health Sci."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Xu, X.-Y., Meng, X., Li, S., Gan, R.-Y., Li, Y., and Li, H.-B. (2018). Bioactivity, Health Benefits, and Related Molecular Mechanisms of Curcumin: Current Progress, Challenges, and Perspectives. Nutrients, 10.","DOI":"10.3390\/nu10101553"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2289","DOI":"10.1002\/jbm.a.37778","article-title":"Doxycycline-loaded carboxymethyl cellulose\/sodium alginate\/gelatin hydrogel: An approach for enhancing pressure ulcer healing in a rat model","volume":"112","author":"Aldaghi","year":"2024","journal-title":"J. Biomed. Mater. Res. Part A"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"135425","DOI":"10.1016\/j.ijbiomac.2024.135425","article-title":"A multifunctional polyacrylonitrile fibers\/alginate-based hydrogel loaded with chamomile extract and silver sulfadiazine for full-thickness wound healing","volume":"279","author":"Moshfeghi","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"34177","DOI":"10.1007\/s11356-018-3850-9","article-title":"Utilization of extracts of Musa paradisica (banana) peels and Dolichos lablab (Indian bean) seeds as low-cost natural coagulants for turbidity removal from water","volume":"26","author":"Daverey","year":"2019","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"106118","DOI":"10.1016\/j.jddst.2024.106118","article-title":"Evaluation of scarless wound healing through nanohydrogel infused with selected plant extracts","volume":"100","author":"Mercy","year":"2024","journal-title":"J. Drug Deliv. Sci. Technol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"108439","DOI":"10.1016\/j.inoche.2020.108439","article-title":"Green synthesis of nanoparticles using Calendula officinalis extract from silver sulfate and their antibacterial effects on Pectobacterium caratovorum","volume":"125","author":"Olfati","year":"2021","journal-title":"Inorg. Chem. Commun."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"977","DOI":"10.1007\/s10735-024-10245-x","article-title":"Harmony in nature\u2019s elixir: A comprehensive exploration of ethanol and nano-formulated extracts from Passiflora incarnata leaves: Unveiling in vitro cytotoxicity, acute and sub-acute toxicity profiles in Swiss albino mice","volume":"55","author":"Deepika","year":"2024","journal-title":"J. Mol. Histol."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Janani, G., Girigoswami, A., Deepika, B., Udayakumar, S., and Girigoswami, K. (2024). Unveiling the Role of Nano-Formulated Red Algae Extract in Cancer Management. Molecules, 29.","DOI":"10.3390\/molecules29092077"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2679","DOI":"10.1007\/s00289-019-02873-1","article-title":"The potential use of cross-linked alginate\/gelatin hydrogels containing silver nanoparticles for wound dressing applications","volume":"77","author":"Baukum","year":"2020","journal-title":"Polym. Bull."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2400054","DOI":"10.1002\/adtp.202400054","article-title":"Hydrogels of Alginate Derivative-Encased Nanodots Featuring Carbon-Coated Manganese Ferrite Cores with Gold Shells to Offer Antiangiogenesis with Multimodal Imaging-Based Theranostics","volume":"7","author":"Gowtham","year":"2024","journal-title":"Adv. Ther."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"2891","DOI":"10.1016\/j.xphs.2023.08.014","article-title":"Combinatorial effect of Doxorubicin entrapped in Alginate-Chitosan hybrid polymer and Cerium oxide Nanocomposites on skin cancer management in mice","volume":"112","author":"Shurfa","year":"2023","journal-title":"J. Pharm. Sci."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Harini, K., Alomar, S.Y., Vajagathali, M., Manoharadas, S., Thirumalai, A., Girigoswami, K., and Girigoswami, A. (2024). Niosomal Bupropion: Exploring Therapeutic Frontiers through Behavioral Profiling. Pharmaceuticals, 17.","DOI":"10.3390\/ph17030366"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"10406","DOI":"10.1038\/s41598-024-59842-6","article-title":"Photocatalytic degradation of antibiotics and antimicrobial and anticancer activities of two-dimensional ZnO nanosheets","volume":"14","author":"Bhuin","year":"2024","journal-title":"Sci. Rep."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/29\/21\/5004\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:18:10Z","timestamp":1760113090000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/29\/21\/5004"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,22]]},"references-count":50,"journal-issue":{"issue":"21","published-online":{"date-parts":[[2024,11]]}},"alternative-id":["molecules29215004"],"URL":"https:\/\/doi.org\/10.3390\/molecules29215004","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,22]]}}}