{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T02:17:24Z","timestamp":1792376244471,"version":"4.4.0"},"reference-count":47,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2024,11,7]],"date-time":"2024-11-07T00:00:00Z","timestamp":1730937600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Project of ESI Discipline Construction of Changsha Medical College","award":["2022CYY018"],"award-info":[{"award-number":["2022CYY018"]}]},{"name":"Project of ESI Discipline Construction of Changsha Medical College","award":["2022YFE0105700"],"award-info":[{"award-number":["2022YFE0105700"]}]},{"name":"Project of ESI Discipline Construction of Changsha Medical College","award":["2022B02021"],"award-info":[{"award-number":["2022B02021"]}]},{"name":"National key R &amp; D program of China","award":["2022CYY018"],"award-info":[{"award-number":["2022CYY018"]}]},{"name":"National key R &amp; D program of China","award":["2022YFE0105700"],"award-info":[{"award-number":["2022YFE0105700"]}]},{"name":"National key R &amp; D program of China","award":["2022B02021"],"award-info":[{"award-number":["2022B02021"]}]},{"name":"Key R&amp;D Program of Xinjiang Uygur Autonomous Region","award":["2022CYY018"],"award-info":[{"award-number":["2022CYY018"]}]},{"name":"Key R&amp;D Program of Xinjiang Uygur Autonomous Region","award":["2022YFE0105700"],"award-info":[{"award-number":["2022YFE0105700"]}]},{"name":"Key R&amp;D Program of Xinjiang Uygur Autonomous Region","award":["2022B02021"],"award-info":[{"award-number":["2022B02021"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Suitable fermentation substrates and fermentation modes can effectively improve the fermentation ethanol yield. In this study, we optimised the hydrothermal pretreatment conditions by orthogonal optimisation using waste tissue paper as substrate. These conditions consisted of 50 min duration in a high-pressure reactor with pure water as solvent at a temperature of 160 \u00b0C. The biomass to water ratio was maintained at a constant level. The cellulose content of the pretreated TP was 81.19 \u00b1 4.06%, which was an increase of 21.59% compared to the blank control. The 72 h reducing sugar yield of pretreated TP was 0.61 g sugar\/g paper, which was 38.64% higher than that of untreated TP. Subsequently, the pretreated TP was fermented under optimal conditions. The mixed group of Saccharomyces cerevisiae and Candida shehatae (SC) showed a distributed saccharification fermentation pattern, with an ethanol yield of 28.11 g\/L in 72 h. On the other hand, the single Saccharomyces cerevisiae (S) exhibited a homobloc saccharification fermentation pattern, with an ethanol yield of 35.15 g\/L in 72 h.<\/jats:p>","DOI":"10.3390\/molecules29225266","type":"journal-article","created":{"date-parts":[[2024,11,7]],"date-time":"2024-11-07T06:41:22Z","timestamp":1730961682000},"page":"5266","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":106,"title":["Optimisation of the Ethanol Fermentation Process Using Hydrothermal Pretreatment of Cellulose Waste\u2014Effect of Fermentation Pattern and Strain"],"prefix":"10.3390","volume":"29","author":[{"given":"Jun","family":"Zhou","sequence":"first","affiliation":[{"name":"School of Management, Changsha Medical University, Changsha 410219, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pin","family":"Lv","sequence":"additional","affiliation":[{"name":"Department of Environmental Science and Engineering, University of Science and Technology Beijing, Beijing Key Laboratory of Resource-Oriented Treatment of Industrial Pollutants, Beijing 100083, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Binsheng","family":"He","sequence":"additional","affiliation":[{"name":"School of Management, Changsha Medical University, Changsha 410219, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjing","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Management, Changsha Medical University, Changsha 410219, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2093-6191","authenticated-orcid":false,"given":"Gao","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Management, Changsha Medical University, Changsha 410219, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7402-4097","authenticated-orcid":false,"given":"Hongzhi","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Environmental Science and Engineering, University of Science and Technology Beijing, Beijing Key Laboratory of Resource-Oriented Treatment of Industrial Pollutants, Beijing 100083, China"},{"name":"Xinjiang Key Laboratory of Clean Conversion and High Value Utilization of Biomass Resources, School of Resource and Environmental Science, Yili Normal University, Yining 835000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yueyao","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Environmental Science and Engineering, University of Science and Technology Beijing, Beijing Key Laboratory of Resource-Oriented Treatment of Industrial Pollutants, Beijing 100083, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guiyun","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Management, Changsha Medical University, Changsha 410219, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,11,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"26265","DOI":"10.1016\/j.ijhydene.2022.06.224","article-title":"Key prospects and major development of hydrogen and bioethanol production","volume":"47","author":"Khan","year":"2022","journal-title":"Int. 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