{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,4]],"date-time":"2026-04-04T13:48:40Z","timestamp":1775310520201,"version":"3.50.1"},"reference-count":54,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2024,11,7]],"date-time":"2024-11-07T00:00:00Z","timestamp":1730937600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"\u201cConseil R\u00e9gional de Bourgogne Franche-Comt\u00e9\u201d","award":["ANR-17-EURE-0002"],"award-info":[{"award-number":["ANR-17-EURE-0002"]}]},{"name":"\u201cConseil R\u00e9gional de Bourgogne Franche-Comt\u00e9\u201d","award":["ANR-15-IDEX-0003"],"award-info":[{"award-number":["ANR-15-IDEX-0003"]}]},{"name":"EIPHI Graduate School","award":["ANR-17-EURE-0002"],"award-info":[{"award-number":["ANR-17-EURE-0002"]}]},{"name":"EIPHI Graduate School","award":["ANR-15-IDEX-0003"],"award-info":[{"award-number":["ANR-15-IDEX-0003"]}]},{"name":"French \u201cInvestissements d\u2019Avenir\u201d program","award":["ANR-17-EURE-0002"],"award-info":[{"award-number":["ANR-17-EURE-0002"]}]},{"name":"French \u201cInvestissements d\u2019Avenir\u201d program","award":["ANR-15-IDEX-0003"],"award-info":[{"award-number":["ANR-15-IDEX-0003"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>To obtain versatile nanoplatforms comparable for various bio-applications, synthesis and functionalization of two inorganic nanoparticles (NPs), i.e., gold (AuNPs) and iron oxide (SPIONs), are described for different NP diameters. Chosen ligands have adapted chemical function to graft to the surfaces of the NPs (thiols and phosphonates, respectively) and the identical frequently used external carboxyl group for comparison of the NPs\u2019 material effect on their final behavior. To further evaluate molecular length effect, AuNPs are functionalized by different ligands. Numerous characterizations highlight the colloidal stability when grafting organic molecules on NPs. The potentiality of the functionalized NPs to react efficiently with a protein monolayer is finally evaluated by grafting them on a protein covered chip, characterized by atomic force microscopy. Comparison of the NPs\u2019 surface densities and measured heights enable observation of different NPs\u2019 reactivity and infer the influence of the inorganic core material, as well as the NPs\u2019 size and ligand length. AuNPs have higher affinities to biomolecules, especially when covered by shorter ligands. NP ligands should be chosen not only based on their length but also on their chemical chain, which affects proteic layer interactions. This original multiplex comparison method using AFM is of great interest to screen the effects of used NP materials and functionalization when developing theranostic nanoplatforms.<\/jats:p>","DOI":"10.3390\/molecules29225270","type":"journal-article","created":{"date-parts":[[2024,11,7]],"date-time":"2024-11-07T09:24:13Z","timestamp":1730971453000},"page":"5270","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Multiplex Evaluation of Biointerface-Targeting Abilities and Affinity of Synthetized Nanoparticles\u2014A Step Towards Improved Nanoplatforms for Biomedical Applications"],"prefix":"10.3390","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3355-8257","authenticated-orcid":false,"given":"M\u00e9lanie","family":"Romain","sequence":"first","affiliation":[{"name":"Laboratoire Interdisciplinaire Carnot de Bourgogne, UMR 6303 CNRS\u2014Universit\u00e9 de Bourgogne, 21078 Dijon, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C\u00e9line","family":"Elie-Caille","sequence":"additional","affiliation":[{"name":"Institut FEMTO-ST, UMR 6174 CNRS\u2014Universit\u00e9 de Franche-Comt\u00e9, 25030 Besanc\u0327on, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dorra","family":"Ben Elkadhi","sequence":"additional","affiliation":[{"name":"Laboratoire Interdisciplinaire Carnot de Bourgogne, UMR 6303 CNRS\u2014Universit\u00e9 de Bourgogne, 21078 Dijon, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olivier","family":"Heintz","sequence":"additional","affiliation":[{"name":"Laboratoire Interdisciplinaire Carnot de Bourgogne, UMR 6303 CNRS\u2014Universit\u00e9 de Bourgogne, 21078 Dijon, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-9750-4228","authenticated-orcid":false,"given":"Micha\u00eble","family":"Herbst","sequence":"additional","affiliation":[{"name":"Laboratoire Interdisciplinaire Carnot de Bourgogne, UMR 6303 CNRS\u2014Universit\u00e9 de Bourgogne, 21078 Dijon, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6346-7623","authenticated-orcid":false,"given":"Lionel","family":"Maurizi","sequence":"additional","affiliation":[{"name":"Laboratoire Interdisciplinaire Carnot de Bourgogne, UMR 6303 CNRS\u2014Universit\u00e9 de Bourgogne, 21078 Dijon, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wilfrid","family":"Boireau","sequence":"additional","affiliation":[{"name":"Institut FEMTO-ST, UMR 6174 CNRS\u2014Universit\u00e9 de Franche-Comt\u00e9, 25030 Besanc\u0327on, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0127-3858","authenticated-orcid":false,"given":"Nadine","family":"Millot","sequence":"additional","affiliation":[{"name":"Laboratoire Interdisciplinaire Carnot de Bourgogne, UMR 6303 CNRS\u2014Universit\u00e9 de Bourgogne, 21078 Dijon, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,11,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1252","DOI":"10.1016\/j.addr.2008.03.018","article-title":"Magnetic Nanoparticles in MR Imaging and Drug Delivery","volume":"60","author":"Sun","year":"2008","journal-title":"Adv. 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