{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T16:41:24Z","timestamp":1790700084466,"version":"4.1.0"},"reference-count":49,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2024,11,28]],"date-time":"2024-11-28T00:00:00Z","timestamp":1732752000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Medical University Lodz","award":["503\/1-079-01\/503-11-001"],"award-info":[{"award-number":["503\/1-079-01\/503-11-001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Numerous compounds that are scavengers of hydroxyl radicals (\u2022OH) in Fenton systems have low solubility in water. Therefore, they are dissolved in organic solvents to reach suitable concentrations in the reaction milieu of the Fenton system. However, these solvents may react with \u2022OH and iron, leading to significant errors in the results. We evaluated 11 solvents (4 alcohols, acetone, 4 esters, dimethyl-sulfoxide, and acetonitrile) at concentrations ranging from 0.105 \u00b5mol\/L to 0.42 \u00b5mol\/L to assess their effects on light emission, a recognized measure of \u2022OH radical activity, in the Fe2+-EGTA-H2O2 system. Six solvents inhibited and four solvents enhanced light emission at all tested concentrations. Acetonitrile, which initially suppressed light emission, lost this effect at a concentration of 0.105 \u00b5mol\/L, (\u22121 \u00b1 13 (2; 0) %, p &gt; 0.05). Methanol, at the lowest tested concentration, inhibited light emission by 62 \u00b1 4% (p &lt; 0.05), while butyl butyrate enhanced it by 93 \u00b1 16% (p &lt; 0.05). These effects may be explained by solvent-driven \u2022OH-scavenging, inhibition or acceleration of Fe2+ regeneration, or photon emission from excited solvent molecules. Our findings suggest that acetonitrile seems suitable for preparing stock solutions to evaluate antioxidant activity in the Fe2+-EGTA-H2O2 system, provided that the final concentration of this solvent in the reaction milieu is kept below 0.105 \u00b5mol\/L.<\/jats:p>","DOI":"10.3390\/molecules29235635","type":"journal-article","created":{"date-parts":[[2024,11,28]],"date-time":"2024-11-28T07:28:01Z","timestamp":1732778881000},"page":"5635","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Effect of Selected Organic Solvents on Hydroxyl Radical-Dependent Light Emission in the Fe2+-EGTA-H2O2 System"],"prefix":"10.3390","volume":"29","author":[{"given":"Krzysztof","family":"Sasak","sequence":"first","affiliation":[{"name":"Department of Medical Imaging Techniques, Medical University of Lodz, Lindleya 6, 90-131 Lodz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Micha\u0142","family":"Nowak","sequence":"additional","affiliation":[{"name":"Radiation Protection, University Hospital No 2, Medical University of Lodz, Zeromskiego 113, 90-549 Lodz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Wlodarczyk","sequence":"additional","affiliation":[{"name":"Department of Sleep Medicine and Metabolic Disorders, Medical University of Lodz, Mazowiecka 6\/8, 92-215 Lodz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agata","family":"Sarniak","sequence":"additional","affiliation":[{"name":"Department of Clinical Physiology, Medical University of Lodz, Mazowiecka 6\/8, 92-215 Lodz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3445-6930","authenticated-orcid":false,"given":"Dariusz","family":"Nowak","sequence":"additional","affiliation":[{"name":"Department of Clinical Physiology, Medical University of Lodz, Mazowiecka 6\/8, 92-215 Lodz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,11,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1865","DOI":"10.1016\/S0024-3205(99)00439-7","article-title":"Chemistry, physiology and pathology of free radicals","volume":"65","author":"Bergendi","year":"1999","journal-title":"Life Sci."},{"key":"ref_2","first-page":"13659","article-title":"Reactive oxygen species: From health to disease","volume":"142","author":"Brieger","year":"2012","journal-title":"Swiss Med. Wkly."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/j.saa.2018.01.026","article-title":"Structural and immunological characterisation of hydroxyl radical modified human IgG: Clinical correlation in rheumatoid arthritis","volume":"194","author":"Islam","year":"2018","journal-title":"Spectrochim. Acta A Mol. Biomol. Spectrosc."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1007\/s11010-010-0563-x","article-title":"Metals, oxidative stress and neurodegenerative disorders","volume":"345","author":"Jomova","year":"2010","journal-title":"Mol. Cell Biochem."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.tox.2011.03.001","article-title":"Advances in metal-induced oxidative stress and human disease","volume":"283","author":"Jomova","year":"2011","journal-title":"Toxicology"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1016\/S1570-0232(02)00620-7","article-title":"Hydroxyl radical in living systems and its separation methods","volume":"781","author":"Cheng","year":"2002","journal-title":"J. Chromatogr. B Analyt. Technol. Biomed. Life Sci."},{"key":"ref_7","first-page":"1","article-title":"Are antioxidants helpful for disease prevention?","volume":"1","author":"Hajhashemi","year":"2010","journal-title":"Res. Pharm. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"21138","DOI":"10.3390\/molecules201219753","article-title":"Antioxidant phytochemicals for the prevention and treatment of chronic diseases","volume":"20","author":"Zhang","year":"2015","journal-title":"Molecules"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1515\/pac-2020-0902","article-title":"Methods to evaluate the scavenging activity of antioxidants toward reactive oxygen and nitrogen species (IUPAC Technical Report)","volume":"94","author":"Apak","year":"2022","journal-title":"Pure Appl. Chem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1515\/hfsg.1997.51.6.557","article-title":"Detection of hydroxyl radicals by a chemiluminescence method\u2014A critical review","volume":"51","author":"Backa","year":"1997","journal-title":"Holzforschung"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Ran, Y., Moursy, M., Hider, R.C., and Cilibrizzi, A. (2023). The colourimetric detection of the hydroxyl radical. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24044162"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"5321","DOI":"10.1039\/C7RA13209C","article-title":"Towards reliable quantification of hydroxyl radicals in the Fenton reaction using chemical probes","volume":"8","author":"Rutely","year":"2018","journal-title":"RSC Adv."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1002\/jctb.4634","article-title":"Insight on the fundamentals of advanced oxidation processes. Role and review of the determination methods of reactive oxygen species","volume":"90","author":"Vallejo","year":"2015","journal-title":"J. Chem. Technol. Biotechnol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2976","DOI":"10.1039\/C6CS00192K","article-title":"Recent progress in the development of fluorescent, luminescent and colourimetric probes for detection of reactive oxygen and nitrogen species","volume":"45","author":"Chen","year":"2016","journal-title":"Chem. Soc. Rev."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"132531","DOI":"10.1016\/j.foodchem.2022.132531","article-title":"Plant flavonoids: Classification, distribution, biosynthesis, and antioxidant activity","volume":"383","author":"Shen","year":"2022","journal-title":"Food Chem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"270","DOI":"10.4161\/oxim.2.5.9498","article-title":"Plant polyphenols as dietary antioxidants in human health and disease","volume":"5","author":"Pandey","year":"2009","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Rana, A., Samtiya, M., Dhewa, T., Mishra, V., and Aluko, R.E. (2022). Health benefits of polyphenols: A concise review. J. Food. Biochem., 46.","DOI":"10.1111\/jfbc.14264"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2862","DOI":"10.1007\/s13197-012-0759-z","article-title":"Extraction and quantification of phenolic acids and flavonols from Eugenia pyriformis using different solvents","volume":"5","author":"Haminiuk","year":"2014","journal-title":"J. Food Sci. Technol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Nowak, M., Tryniszewski, W., Sarniak, A., Wlodarczyk, A., Nowak, P.J., and Nowak, D. (2018). Light emission from the Fe2+-EGTA-H2O2 system: Possible application for the determination of antioxidant activity of plant phenolics. Molecules, 23.","DOI":"10.3390\/molecules23040866"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Nowak, M., Tryniszewski, W., Sarniak, A., Wlodarczyk, A., Nowak, P.J., and Nowak, D. (2022). Concentration dependence of the anti- and pro-oxidant activity of polyphenols as evaluated with a light-emitting Fe2+-EGTA-H2O2 system. Molecules, 27.","DOI":"10.3390\/molecules27113453"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Nowak, M., Tryniszewski, W., Sarniak, A., Wlodarczyk, A., Nowak, P.J., and Nowak, D. (2021). Effect of physiological concentrations of vitamin C on the inhibition of hydroxyl radical-induced light emission from Fe2+-EGTA-H2O2 and Fe3+-EGTA-H2O2 systems In vitro. Molecules, 26.","DOI":"10.3390\/molecules26071993"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"27885","DOI":"10.1039\/C8CP05411H","article-title":"Water Catalysis of the reaction between hydroxyl radicals and linear saturated alcohols (ethanol and n-propanol) at 294 K","volume":"20","author":"Garavagno","year":"2018","journal-title":"Phys. Chem. Chem. Phys."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1111\/j.1530-0277.1985.tb05525.x","article-title":"Ethanol oxidation by hydroxyl radicals: Role of iron chelates, superoxide, and hydrogen peroxide","volume":"9","author":"Feierman","year":"1985","journal-title":"Alcohol. Clin. Exp. Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"13051","DOI":"10.1021\/cr500310b","article-title":"Environmental implications of hydroxyl radicals ((\u2022)OH)","volume":"115","author":"Gligorovski","year":"2015","journal-title":"Chem. Rev."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1016\/S0969-806X(03)00310-4","article-title":"\u2022OH reactions with aliphatic alcohols: Evaluation of kinetics by direct optical absorption measurement. A pulse radiolysis study","volume":"67","author":"Alam","year":"2003","journal-title":"Radiat. Phys. Chem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/S0166-1280(03)00248-3","article-title":"A QSAR study on the kinetics of the reactions of aliphatic alcohols with the photogenerated hydroxyl radicals","volume":"631","year":"2003","journal-title":"J. Mol. Struct. THEOCHEM"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2622","DOI":"10.1039\/b101385h","article-title":"A pulse radiolysis study of H atom reactions with aliphatic alcohols: Evaluation of kinetics by direct optical absorption measurement","volume":"3","author":"Alam","year":"2001","journal-title":"Phys. Chem. Chem. Phys."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"3386","DOI":"10.1021\/acs.est.1c03796","article-title":"Overlooked formation of H2O2 during the hydroxyl radical-scavenging process when using alcohols as scavengers","volume":"56","author":"Wang","year":"2022","journal-title":"Environ. Sci. Technol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"397","DOI":"10.3184\/146867817X14954764850496","article-title":"Strong Inhibition of the Fe3+ + H2O2 reaction by ethanol: Evidence against the free radical theory","volume":"42","author":"Kremer","year":"2017","journal-title":"Prog. React. Kinet. Mech."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"109185","DOI":"10.1016\/j.cclet.2023.109185","article-title":"Complexes of cupric ion and tartaric acid enhanced calcium peroxide Fenton-like reaction for metronidazole degradation","volume":"35","author":"Pan","year":"2024","journal-title":"Chin. Chem. Lett."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1016\/j.exger.2008.01.010","article-title":"DCFH2 interactions with hydroxyl radicals and other oxidants\u2014Influence of organic solvents","volume":"43","author":"Silber","year":"2008","journal-title":"Exp. Gerontol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1016\/0003-9861(90)90288-A","article-title":"Quantitation of the hydroxyl radical by reaction with dimethyl sulfoxide","volume":"278","author":"Steiner","year":"1990","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/0891-5849(94)E0139-A","article-title":"Oxidation of DMSO and methanesulfinic acid by the hydroxyl radical","volume":"18","author":"Scaduto","year":"1995","journal-title":"Free Radic. Biol. Med."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"3182","DOI":"10.1021\/es304600z","article-title":"Atmospheric chemistry of benzyl alcohol: Kinetics and mechanism of reaction with OH radicals","volume":"47","author":"Bernard","year":"2013","journal-title":"Environ. Sci. Technol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1039\/p29930000289","article-title":"Oxidation of benzene by the OH radical. A product and pulse radiolysis study in oxygenated aqueous solution","volume":"2","author":"Pan","year":"1993","journal-title":"J. Chem. Soc. Perkin Trans."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1232","DOI":"10.1021\/j100156a037","article-title":"Kinetics and mechanism of the reaction of hydroxyl radicals with acetonitrile under atmospheric conditions","volume":"95","author":"Hynes","year":"1991","journal-title":"J. Phys. Chem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"5086","DOI":"10.1021\/jp0708345","article-title":"Mechanism of OH radical reactions with HCN and CH3CN: OH regeneration in the presence of O2","volume":"111","author":"Galano","year":"2007","journal-title":"J. Phys. Chem. A"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1002\/kin.550250704","article-title":"Stone: Kinetics of the reactions of OH radicals with selected acetates and other esters under simulated atmospheric conditions","volume":"25","author":"Williams","year":"1993","journal-title":"Int. J. Chem. Kinet."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1002\/kin.550200210","article-title":"The gas phase reactions of hydroxyl radicals with a series of esters over the temperature range 240\u2013440 K","volume":"20","author":"Wallington","year":"1988","journal-title":"J. Chem. Kinet."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"8146","DOI":"10.3390\/ijms12118146","article-title":"Protective effect of the ethyl acetate fraction of Sargassum muticum against ultraviolet B-irradiated damage in human keratinocytes","volume":"12","author":"Piao","year":"2011","journal-title":"Int. J. Mol. Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"7763","DOI":"10.1021\/acs.est.8b00582","article-title":"Elucidating the elementary reaction pathways and kinetics of hydroxyl radical-induced acetone degradation in aqueous phase advanced oxidation processes","volume":"52","author":"Kamath","year":"2018","journal-title":"Environ. Sci. Technol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"5021","DOI":"10.1021\/jp0273023","article-title":"Reaction of hydroxyl radical with acetone. 2. products and reaction mechanism","volume":"107","author":"Ranajit","year":"2003","journal-title":"J. Phys. Chem. A"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1016\/j.jssc.2012.07.019","article-title":"From dihydrated iron(III) phosphate to monohydrated ammonium\u2013iron(II) phosphate: Solvothermal reaction mediated by acetone\u2013urea mixtures","volume":"196","author":"Trobajo","year":"2012","journal-title":"J. Solid State Chem."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1007\/BF03051288","article-title":"The photo-reduction of ferric chloride in the presence of aqueous acetone and anhydrous ether","volume":"18","author":"Prasad","year":"1943","journal-title":"Proc. Indian Acad. Sci."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1007\/BF01672410","article-title":"Photochemical reduction of iron trichloride in ethyl acetate: Synthesis, M\u00f6ssbauer spectra and the crystal structure at 80 K of hexakis(ethyl acetate)iron(II) bis-tetrachloroironate(III)","volume":"24","author":"Szafert","year":"1994","journal-title":"J. Chem. Crystallogr."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"3858","DOI":"10.1021\/ja01524a016","article-title":"The photolysis and fluorescence of acetone and acetone-biacetyl mixtures","volume":"81","author":"Heicklen","year":"1959","journal-title":"J. Am. Chem. Soc."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Sasak, K., Nowak, M., Wlodarczyk, A., Sarniak, A., Tryniszewski, W., and Nowak, D. (2024). Light emission from Fe2+-EGTA-H2O2 system depends on the pH of the reaction milieu within the range that may occur in cells of the human body. Molecules, 29.","DOI":"10.3390\/molecules29174014"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"230S","DOI":"10.1093\/ajcn\/81.1.230S","article-title":"Bioavailability and bioefficacy of polyphenols in humans. I. Review of 97 bioavailability studies","volume":"81","author":"Manach","year":"2005","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"8006","DOI":"10.1021\/jf060783t","article-title":"Bioavailability of genistein and its glycoside genistin as measured in the portal vein of freely moving unanesthetized rats","volume":"54","author":"Steensma","year":"2006","journal-title":"J. Agric. Food Chem."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/29\/23\/5635\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:41:40Z","timestamp":1760114500000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/29\/23\/5635"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,28]]},"references-count":49,"journal-issue":{"issue":"23","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["molecules29235635"],"URL":"https:\/\/doi.org\/10.3390\/molecules29235635","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,28]]}}}