{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T20:56:54Z","timestamp":1775077014927,"version":"3.50.1"},"reference-count":56,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2024,12,7]],"date-time":"2024-12-07T00:00:00Z","timestamp":1733529600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University of Rouen Normandy, INSA Rouen Normandy, the Centre National de la Recherche Scientifique (CNRS), European Regional Development Fund (ERDF), Labex SynOrg","award":["ANR-11-LABX-0029"],"award-info":[{"award-number":["ANR-11-LABX-0029"]}]},{"name":"University of Rouen Normandy, INSA Rouen Normandy, the Centre National de la Recherche Scientifique (CNRS), European Regional Development Fund (ERDF), Labex SynOrg","award":["ANR-18-EURE-0020 XL CHEM"],"award-info":[{"award-number":["ANR-18-EURE-0020 XL CHEM"]}]},{"name":"University of Rouen Normandy, INSA Rouen Normandy, the Centre National de la Recherche Scientifique (CNRS), European Regional Development Fund (ERDF), Labex SynOrg","award":["BQI 2022"],"award-info":[{"award-number":["BQI 2022"]}]},{"name":"Carnot Institute I2C, the graduate school for research XL-Chem","award":["ANR-11-LABX-0029"],"award-info":[{"award-number":["ANR-11-LABX-0029"]}]},{"name":"Carnot Institute I2C, the graduate school for research XL-Chem","award":["ANR-18-EURE-0020 XL CHEM"],"award-info":[{"award-number":["ANR-18-EURE-0020 XL CHEM"]}]},{"name":"Carnot Institute I2C, the graduate school for research XL-Chem","award":["BQI 2022"],"award-info":[{"award-number":["BQI 2022"]}]},{"name":"University of Rouen Normandie","award":["ANR-11-LABX-0029"],"award-info":[{"award-number":["ANR-11-LABX-0029"]}]},{"name":"University of Rouen Normandie","award":["ANR-18-EURE-0020 XL CHEM"],"award-info":[{"award-number":["ANR-18-EURE-0020 XL CHEM"]}]},{"name":"University of Rouen Normandie","award":["BQI 2022"],"award-info":[{"award-number":["BQI 2022"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Pentadesma butyracea is a medicinal plant of which bark decoctions are used in traditional medicine for the treatment of diarrhea symptoms in Gabon. The aim of the present work was to perform phytochemical and pharmacological analyses of decoctions of P. butyracea bark. In a principal approach, spectrophotometric analyses were used to quantify phenolic compounds, followed by liquid chromatography coupled to mass spectrometry analysis that allowed the identification of flavanone\u2013flavone dimers as the main metabolites. Pharmacological analyses showed the absence of toxicity, thus confirming the safety of use of this decoction in traditional medicine. The antioxidant activity of the bark decoctions was demonstrated to depend on their phenolic contents. The decoction of stem barks harvested during the rainy season also induced a dose-dependent relaxation of isolated ileum fragments from Wistar rats. In addition, the antidiarrheal activity of P. butyracea barks was investigated against castor oil-induced diarrhea. The oral administration of different concentrations of this decoction led to a decrease in wet stools, indicating an antidiarrheal effect at the doses that were used. These results encourage the deepening of bio-guided research on P. butyracea bark decoctions in order to propose standard traditional medical treatments.<\/jats:p>","DOI":"10.3390\/molecules29235789","type":"journal-article","created":{"date-parts":[[2024,12,9]],"date-time":"2024-12-09T10:11:47Z","timestamp":1733739107000},"page":"5789","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Phytochemical Analysis and Antidiarrheal Activity of Stem Bark Decoctions of Pentadesma butyracea Sabine (Clusiaceae)"],"prefix":"10.3390","volume":"29","author":[{"given":"Ericka","family":"Mayindza Ekaghba","sequence":"first","affiliation":[{"name":"Institut de Pharmacop\u00e9e et M\u00e9decines Traditionnelles (IPHAMETRA), Centre National de la Recherche Scientifique et Technique (CENAREST), Libreville BP 12 141, Gabon"},{"name":"Universit\u00e9 de Rouen Normandie (UNIROUEN), Normandie University, GlycoMEV UR 4358, SFR Normandie V\u00e9g\u00e9tal FED 4277, Innovation Chimie Carnot, IRIB, GDR CNRS Chemobiologie, F-76000 Rouen, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manon","family":"Grenet","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Rouen Normandie (UNIROUEN), Normandie University, Inserm U1245, F-76000 Rouen, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0734-3343","authenticated-orcid":false,"given":"Pierrick","family":"Gandolfo","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Rouen Normandie (UNIROUEN), Normandie University, Inserm U1245, F-76000 Rouen, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3361-1532","authenticated-orcid":false,"given":"Corinne","family":"Loutelier-Bourhis","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Rouen Normandie, INSA Rouen, Normandie University, CNRS, UMR6014\u2014COBRA, F-76000 Rouen, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0665-0074","authenticated-orcid":false,"given":"Isabelle","family":"Schmitz","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Rouen Normandie, INSA Rouen, Normandie University, CNRS, UMR6014\u2014COBRA, F-76000 Rouen, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2406-5664","authenticated-orcid":false,"given":"Carlos","family":"Afonso","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Rouen Normandie, INSA Rouen, Normandie University, CNRS, UMR6014\u2014COBRA, F-76000 Rouen, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrice","family":"Lerouge","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Rouen Normandie (UNIROUEN), Normandie University, GlycoMEV UR 4358, SFR Normandie V\u00e9g\u00e9tal FED 4277, Innovation Chimie Carnot, IRIB, GDR CNRS Chemobiologie, F-76000 Rouen, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Line","family":"Mengome","sequence":"additional","affiliation":[{"name":"Institut de Pharmacop\u00e9e et M\u00e9decines Traditionnelles (IPHAMETRA), Centre National de la Recherche Scientifique et Technique (CENAREST), Libreville BP 12 141, Gabon"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,12,7]]},"reference":[{"key":"ref_1","first-page":"15986","article-title":"Etude \u00e9pid\u00e9miologique des troubles fonctionnels intestinaux dans les structures sanitaires \u00e0 Libreville (Gabon)","volume":"155","year":"2020","journal-title":"J. Appl. Biosc."},{"key":"ref_2","first-page":"75","article-title":"Valorisation socio\u00e9conomique et p\u00e9r\u00e9nnite du Pentadesma butyraceae Sabine en galeries foresti\u00e8res au B\u00e9nin","volume":"12","author":"Sinsin","year":"2003","journal-title":"Cahier Agriculture"},{"key":"ref_3","first-page":"18","article-title":"Connaissances endog\u00e8nes et importance du Pentadesma butyracea (Clusiaceae) pour les populations autochtones au Nord-Ouest B\u00e9nin","volume":"4","author":"Natta","year":"2010","journal-title":"Fruit Veg. Cereal Sci. Biotechnol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"172397","DOI":"10.1155\/2013\/172397","article-title":"Phytochemical screening of Pentadesma butyracea Sabine (Clusiaceae) acclimated in Benin by GC\/MS","volume":"2013","author":"Noudogbessi","year":"2013","journal-title":"ISRN Anal. Chem."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"3137","DOI":"10.1002\/jsfa.7052","article-title":"Profiling the quality characteristics of the butter of Pentadesma butyracea with reference to shea butter","volume":"95","author":"Ayegnon","year":"2015","journal-title":"J. Sci. Food Agric."},{"key":"ref_6","first-page":"143","article-title":"Comparaison des compositions lipidiques des beurres de Pentadesma butyracea et de Karite","volume":"2","author":"Dencausse","year":"1995","journal-title":"OCL Corps Gras Lipides"},{"key":"ref_7","unstructured":"Courtin, O. (1988). Cosmetic Preparation to Retard the Ageing Skin, and Application Process. (Applications. EP0279136A3), European Patent."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Abbiw, D.K. (1990). Useful plants of Ghana, Intermadiate Technology Publications and the Royal Botanic Gardens Kew.","DOI":"10.3362\/9781780443737"},{"key":"ref_9","unstructured":"Adjanohoune, E.J., Ak\u00e9 Assi, L., Chibon, P., de V\u00e9cchy, H., Duboze, E., Eym\u00e9, J., Gassita, J.N., Goudote, E., Guinko, S., and Keita, A. (1984). Contribution Aux \u00c9tudes Ethnobotaniques et Floristiques au Gabon, Agence de Coop\u00e9ration Culturelle et Technique."},{"key":"ref_10","first-page":"151","article-title":"Ethnobotany of Pentadesma butyracea in Benin: A quantitative approach","volume":"9","author":"Avohou","year":"2011","journal-title":"Ethnobot. Res. Appl."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"32","DOI":"10.4314\/cajeb.v12i1.4","article-title":"Innocuity and antioxidant activities of Pentadesma butyracea (1824) leaves for its use in hormone replacement therapy","volume":"12","author":"Tindano","year":"2018","journal-title":"Cameroon J. Exp. Biol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1080\/10412905.2010.9700285","article-title":"Chemical and biological investigation on volatile constituents of Pentadesma butyracea Sabine (Clusiaceae) from Benin","volume":"22","author":"Alitonou","year":"2010","journal-title":"J. Essent. Oil Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"954","DOI":"10.1021\/np8005953","article-title":"Xanthones cytotoxiques et antiplasmodium de Pentadesma butyracea","volume":"72","author":"Guillet","year":"2009","journal-title":"J. Nat. Prod."},{"key":"ref_14","first-page":"1055","article-title":"Xanthones, biflavanones and triterpenes from Pentadesma grandifolia (Clusiaceae): Structural determination and bioactivity","volume":"5","author":"Djoufack","year":"2010","journal-title":"Nat. Prod. Comm."},{"key":"ref_15","first-page":"e01747","article-title":"Pentadesma butyracea in Ghana\u2013indigenous knowledge, uses, and seed characterization","volume":"21","author":"Timtey","year":"2023","journal-title":"Sci. Afr."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.foodchem.2016.12.002","article-title":"Polyphenols from Allanblackia floribunda seeds: Identification, quantification and antioxidant activity","volume":"222","author":"Akpanika","year":"2017","journal-title":"Food Chem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1016\/S1044-0305(01)00226-4","article-title":"Determination of flavone, flavonol, and flavanone aglycones by negative ion liquid chromatography electrospray ion trap mass spectrometry","volume":"12","author":"Fabre","year":"2001","journal-title":"J. Am. Soc. Mass Spectrom."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1556\/1326.2019.00688","article-title":"Characterization and identification of the major flavonoids in Phyllostachys edulis leaf extract by UPLC\u2014QTOF\u2014MS\/MS","volume":"32","author":"Shao","year":"2020","journal-title":"Acta Chromatogr."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"254","DOI":"10.4103\/0973-1296.137365","article-title":"Qualitative and quantitative analysis of the major constituents in traditional Chinese medicine Danmu injection using LC-ESI-MS (n) and LC-DAD","volume":"10","author":"Zhu","year":"2014","journal-title":"Pharmacogn. Mag."},{"key":"ref_20","first-page":"407","article-title":"Cytotoxic constituents of Mallotus Microcarpus","volume":"12","author":"Thai","year":"2017","journal-title":"Nat. Prod. Comm."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Fitzgerald, M., Heinrich, M., and Booker, A. (2020). Medicinal Plant Analysis: A Historical and Regional Discussion of Emergent Complex Techniques. Front. Pharmacol., 10.","DOI":"10.3389\/fphar.2019.01480"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Taher, M.A., Laboni, A.A., Shompa, S.A., Rahman, M.M., Hasan, M.M., Hasnat, H., and Khan, M. (2023). Bioactive compounds extracted from leaves of G. cyanocarpa using various solvents in chromatographic separation showed anti-cancer and anti-microbial potentiality in in silico approach. Chin. J. Anal. Chem, 51.","DOI":"10.1016\/j.cjac.2023.100336"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"656","DOI":"10.5897\/JMPR2017.6469","article-title":"Phytochemical composition, acute toxicity and phytohormonal Garcinia madruno activity of hydroalcoholic extract of Pentadesma butyracea (Clusiaceae Sabine (1824)) seeds","volume":"11","author":"Tindano","year":"2017","journal-title":"J. Med. Plant Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1016\/j.jep.2016.09.033","article-title":"Allanblackia floribunda Oliv.: An aphrodisiac plant with vasorelaxant properties","volume":"192","author":"Brusotti","year":"2016","journal-title":"J. Ethnopharmacol."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Ayegnon, B.P., Chabi, I.B., Akogou, F.U.G., and Kayod\u00e9, A.P.P. (2021). Physicochemical characterization and microbiology quality of the Pentadesma butyracea fruit pulp collected from various parks in Benin. Sci. Rep., 11.","DOI":"10.1038\/s41598-021-96211-z"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1016\/j.jff.2016.10.001","article-title":"Comprehensive characterization and antioxidant activities of the main biflavonoids of: A novel tropical species for developing functional products","volume":"27","author":"Cossio","year":"2016","journal-title":"J. Funct. Foods"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"e35422","DOI":"10.1016\/j.heliyon.2024.e35422","article-title":"Phyto-pharmacological and computational profiling of Bombax ceiba Linn. Leaves revealed pharmacological properties against oxidation, hyperglycemia, pain, and diarrhea","volume":"10","author":"Taher","year":"2024","journal-title":"Heliyon"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"13","DOI":"10.5530\/fra.2017.1.3","article-title":"In vitro antioxidant and RBC membrane stabilization activity of Euphorbia wallichii","volume":"7","author":"Tantary","year":"2017","journal-title":"Free Radic. Antioxid."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1004","DOI":"10.1111\/j.1440-1746.2012.07108.x","article-title":"Oxidative stress in inflammation-based gastrointestinal tract diseases: Challenges and opportunities","volume":"27","author":"Kim","year":"2012","journal-title":"J. Gastroentero.l Hepatol."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Miranda-Bautista, J., Ba\u00f1ares, R., and Vaquero, J. (2017). The Gastrointestinal System: Anatomy and sources of oxidative stress. Gastrointestinal Tissue, Academic Press.","DOI":"10.1016\/B978-0-12-805377-5.00001-1"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"31","DOI":"10.4314\/epj.v36i1.4","article-title":"Antidiarrhoeal activity of the 80% methanol root extract of alictrum ryhnchocarpum Dill. & A. Rich and its major constituent against castor oil-induced diarrhoea in mice","volume":"36","author":"Feyisa","year":"2020","journal-title":"Ethiop. Pharm. J."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1007\/s10298-012-0734-x","article-title":"Potentiel pharmacologique des \u00e9corces de tige de Spondias mombin L. (Anacardiaceae) sur la motricit\u00e9 in vitro du duod\u00e9num de lapin; une plante m\u00e9dicinale utilis\u00e9e dans le traitement traditionnel des troubles digestifs","volume":"10","author":"Diby","year":"2012","journal-title":"Phytoth\u00e9rapie"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"929","DOI":"10.4314\/tjpr.v13i6.15","article-title":"Antidiarrheal activity of aqueous extract of the stem bark of Sapium Ellipticum (Euphorbiaceae)","volume":"13","author":"Wansi","year":"2014","journal-title":"Trop. J. Pharm. Res."},{"key":"ref_34","first-page":"36","article-title":"Antidiarrhea effect of aqueous extracts of Momordica balsamina and Stachytarpheta indica in Rats","volume":"1","author":"Otimenyin","year":"2008","journal-title":"J. Nat. Prod."},{"key":"ref_35","first-page":"11","article-title":"Mise en \u00e9vidence d\u2019une action myorelaxante et cholinolytique de Bitter GG (BGG), un antidiarrh\u00e9ique de source v\u00e9g\u00e9tale","volume":"5","author":"Bahi","year":"2000","journal-title":"Afr. Biomed."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1007\/s10298-010-0600-7","article-title":"Effets pharmacodynamiques d\u2019un extrait hydroalcoolique de Curcuma longa Linn\u00e9 (Zingiberaceae) sur le syst\u00e8me cardiovasculaire, la respiration et l\u2019activit\u00e9 m\u00e9canique intestinale de mammif\u00e8res","volume":"9","author":"Bleu","year":"2011","journal-title":"Phytoth\u00e9rapie"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1453","DOI":"10.1016\/j.biopha.2017.11.147","article-title":"Evidence based traditional anti-diarrheal medicinal plants and their phytocompounds","volume":"96","author":"Rawat","year":"2017","journal-title":"Biomed. Pharmacother."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"5922487","DOI":"10.1155\/2024\/5922487","article-title":"In Vivo Antidiarrheal potential of the leaf extract of Maytenus addat (Loes.) Sebsebe and its major compound","volume":"2024","author":"Mogose","year":"2024","journal-title":"J. Trop. Med."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.1016\/j.vaccine.2010.11.065","article-title":"Diarrhea: Case definition and guidelines for collection, analysis, and presentation of immunization safety data","volume":"29","author":"Gidudu","year":"2011","journal-title":"Vaccine"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"4994","DOI":"10.3390\/molecules25214994","article-title":"Evaluation of various solvent extracts of Tetrastigma leucostaphylum (Dennst.) Alston leaves, a Bangladeshi traditional medicine used for the treatment of Diarrhea","volume":"25","author":"Rudra","year":"2020","journal-title":"Molecules"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1007\/BF00500077","article-title":"Loperamide reduces the intestinal secretion but not the mucosal cAMP accumulation induced by cholera toxin","volume":"317","author":"Farack","year":"1981","journal-title":"Naunyn-Schmiedeberg\u2019s Arch. Pharmacol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/0090-6980(77)90011-9","article-title":"The effect of the loperamide on prostaglandin-induced diarrhea in rat and man","volume":"13","author":"Karim","year":"1977","journal-title":"Prostaglandins"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1093\/toxsci\/62.2.330","article-title":"The frequency of U-shaped dose responses in the toxicological literature","volume":"62","author":"Calabrese","year":"2001","journal-title":"Toxicol. Sci."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1016\/j.biopha.2018.11.131","article-title":"Antidiarrheal activity of \u03b1-terpineol in mice","volume":"110","author":"Nunes","year":"2019","journal-title":"Biomed. Pharmacother."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1016\/j.jep.2015.08.020","article-title":"Antidiarrheal activity of cashew GUM, a complex heteropolysaccharide extracted from exudate of Anacardium occidentale L. in rodents","volume":"174","author":"Costa","year":"2015","journal-title":"J. Ethnopharmacol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/0304-3959(83)90201-4","article-title":"Ethical guidelines for investigations of experimental pain in conscious animals","volume":"16","author":"Zimmermann","year":"1983","journal-title":"Pain"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Singleton, V.L., Orthofer, R., and Lamuela-Ravent\u00f3s, R.M. (1999). Analysis of Total Phenols and Other Oxidation Substrates and Antioxidants by Means of Folin-Ciocalteu Reagent, Methods in Enzymology, Academic Press.","DOI":"10.1016\/S0076-6879(99)99017-1"},{"key":"ref_48","unstructured":"Rib\u00e9reau-Gayon, J., Peynaud, M., Rib\u00e9reau-Gayon, P., and Sudraud, P. (1972). Sciences et Techniques du VIN. Tome 1, Analyse et Controle Des VINs, Dunod, Ed."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.bcdf.2014.02.001","article-title":"Screening of antioxidant activities of polysaccharides extracts from endemic plants in Gabon","volume":"3","author":"Mengome","year":"2014","journal-title":"Bioact. Carbohydr. Diet. Fibre"},{"key":"ref_50","unstructured":"Organisation for Economic Co-operation and Development (OECD) (2002). The Organization of Economic Co-Operation and Development Guidelines Test NO. 423 Acute Oral Toxicity-Acute Toxic Class Method. Guidelines for the Testing of Chemicals, \u00c9ditions OCDE. Section 4."},{"key":"ref_51","first-page":"84","article-title":"The antispasmodic effect of aqueous root bark extract of Carissa edulis (Forssk.) Vahl on isolated rabbit jejunum is mediated through blockade of calcium channels","volume":"7","author":"Oluoch","year":"2020","journal-title":"Discovery Phytomed."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"3037120","DOI":"10.1155\/2018\/3037120","article-title":"Antidiarrheal activity of 80% methanolic leaf extract of Justicia schimperiana","volume":"6","author":"Mekonnen","year":"2018","journal-title":"Evid. -Based Complement. Altern. Med."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Rehman, N.U., Ansari, M.N., and Samad, A. (2020). In silico, ex vivo and in vivo studies of roflumilast as a potential antidiarrheal and antispasmodic agent: Inhibition of the PDE-4 enzyme and voltage-gated Ca++ ion channels. Molecules, 25.","DOI":"10.3390\/molecules25041008"},{"key":"ref_54","unstructured":"The National Academies Press (2011). National Research Council (US) Committee for the Update of the Guide for the Care and Use of Laboratory Animals. Guide for the Care and Use of Laboratory Animals, The National Academies Press. [8th ed.]."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1016\/0090-6980(76)90189-1","article-title":"Enteropooling assay: A test for diarrhea produced by prostaglandins","volume":"11","author":"Robert","year":"1976","journal-title":"Prostaglandins"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1080\/13880200701575304","article-title":"Antidiarrheal Activity of the Aqueous extract of Punica granatum (Pomegranate) peels","volume":"45","author":"Qnais","year":"2007","journal-title":"Pharm. Biol."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/29\/23\/5789\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:49:21Z","timestamp":1760114961000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/29\/23\/5789"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,7]]},"references-count":56,"journal-issue":{"issue":"23","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["molecules29235789"],"URL":"https:\/\/doi.org\/10.3390\/molecules29235789","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,7]]}}}