{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T10:52:24Z","timestamp":1788173544227,"version":"build-2803163510"},"reference-count":107,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2024,12,18]],"date-time":"2024-12-18T00:00:00Z","timestamp":1734480000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100016981","name":"Necmettin Erbakan University Scientific Research Rrojects Unit","doi-asserted-by":"publisher","award":["201215001"],"award-info":[{"award-number":["201215001"]}],"id":[{"id":"10.13039\/501100016981","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>In the present study, ultra-small, magnetic, oleyl amine-coated Fe3O4 nanoparticles were synthesized and stabilized with a cationic ligand, cetyltrimethylammonium bromide, and an anticancer drug, methotrexate, was incorporated into a micelle-like nanoparticle structure for glioblastoma treatment. Nanoparticles were further characterized for their physicochemical properties using spectroscopic methods. Drug incorporation efficiency, drug loading, and drug release profile of the nanoparticles were investigated. According to the results, max incorporation efficiency% of 89.5 was found for 25 \u00b5g\/mL of methotrexate-loaded nanoparticles. The cumulative amount of methotrexate released reached 40% at physiological pH and 85% at a pH of 5.0 up to 12 h. The toxicity and anticancer efficacy of the nanoparticles were also studied on U87 cancer and L929 cells. IC50 concentration of nanoparticles reduced cell viability to 49% in U87 and 72% in L929 cells. The cellular uptake of nanoparticles was found to be 1.92-fold higher in U87 than in L929 cells. The total apoptosis% in U87 cells was estimated to be ~10-fold higher than what was observed in the L929 cells. Nanoparticles also inhibited the cell motility and prevented the metastasis of U87 cell lines. Overall, designed nanoparticles are a promising controlled delivery system for methotrexate to the cancer cells to achieve better therapeutic outcomes.<\/jats:p>","DOI":"10.3390\/molecules29245977","type":"journal-article","created":{"date-parts":[[2024,12,18]],"date-time":"2024-12-18T09:43:03Z","timestamp":1734514983000},"page":"5977","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Cationic Micelle-like Nanoparticles as the Carrier of Methotrexate for Glioblastoma Treatment"],"prefix":"10.3390","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5516-3603","authenticated-orcid":false,"given":"Tu\u011fba Nur","family":"Aslan","sequence":"first","affiliation":[{"name":"Department of Molecular Biology and Genetics, Faculty of Arts and Science, Necmettin Erbakan University, Konya 42090, Turkey"},{"name":"Science and Technology Research and Application Center (BITAM), Necmettin Erbakan University, Konya 42140, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,12,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1007\/s11060-016-2331-6","article-title":"Impact of Concurrent Chemotherapy with Radiation Therapy for Elderly Patients with Newly Diagnosed Glioblastoma: A Review of the National Cancer Data Base","volume":"131","author":"Huang","year":"2017","journal-title":"J. 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