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Cooperative Research Grant(s) of NEKKEN, 2020 and 2021","award":["16H05174"],"award-info":[{"award-number":["16H05174"]}]},{"name":"Health and Labor Sciences Research Grants: the Cooperative Research Grant(s) of NEKKEN, 2020 and 2021","award":["17H04661"],"award-info":[{"award-number":["17H04661"]}]},{"name":"Health and Labor Sciences Research Grants: the Cooperative Research Grant(s) of NEKKEN, 2020 and 2021","award":["24K01930"],"award-info":[{"award-number":["24K01930"]}]},{"name":"Health and Labor Sciences Research Grants: the Cooperative Research Grant(s) of NEKKEN, 2020 and 2021","award":["2020-Ippan-09"],"award-info":[{"award-number":["2020-Ippan-09"]}]},{"name":"Health and Labor Sciences Research Grants: the Cooperative Research Grant(s) of NEKKEN, 2020 and 2021","award":["2020-Ippan-24"],"award-info":[{"award-number":["2020-Ippan-24"]}]},{"name":"Health and Labor Sciences Research Grants: the Cooperative Research Grant(s) of NEKKEN, 2020 and 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This study evaluated the potential of 111In-labeled anti-SFTSV antibodies targeting SFTSV structural proteins as single-photon emission computed tomography (SPECT) imaging agents for the selective visualization of SFTSV-infected sites. This study used nuclear medicine imaging to elucidate the pathology of SFTS and assess its therapeutic efficacy. Immunostaining experiments confirmed that the anti-SFTSV antibody (N-mAb), which targets the N protein, specifically accumulated in SFTSV-infected Vero E6 cells. 111In-labeled N-mAb was successfully prepared using a diethylenetriaminepentaacetic acid (DTPA) chelator, resulting in [111In]In-DTPA-N-mAb with high radiochemical purity exceeding 95% and a radiochemical yield of 55%. Cell-binding assays using SFTSV-infected Vero E6 cells demonstrated that [111In]In-DTPA-N-mAb binding was detectable even without membrane permeabilization, with the binding intensity correlating with infection levels. In vivo studies using SFTSV-infected A129 mice showed high spleen accumulation of [111In]In-DTPA-N-mAb (87.5% ID\/g), consistent with SFTSV tropism, compared to 12.3% ID\/g in mock-infected mice. SPECT\/CT imaging clearly revealed high radioactivity in these regions. Although nonspecific accumulation was noted in the liver and spleen, this issue may be mitigated through antibody modifications such as fragmentation or PEGylation. Overall, [111In]In-DTPA-N-mAb is a promising imaging agent for non-invasive visualization of SFTSV-infected sites and may aid in elucidating SFTS pathology and assessing therapeutic efficacy.<\/jats:p>","DOI":"10.3390\/molecules30010038","type":"journal-article","created":{"date-parts":[[2024,12,26]],"date-time":"2024-12-26T21:13:18Z","timestamp":1735247598000},"page":"38","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Development of 111In-Labeled Monoclonal Antibodies Targeting SFTSV Structural Proteins for Molecular Imaging of SFTS Infectious Diseases by SPECT"],"prefix":"10.3390","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8141-1212","authenticated-orcid":false,"given":"Takeshi","family":"Fuchigami","sequence":"first","affiliation":[{"name":"Laboratory of Clinical Analytical Sciences, Graduate School of Medical Sciences, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8986-6307","authenticated-orcid":false,"given":"Mya Myat","family":"Ngwe Tun","sequence":"additional","affiliation":[{"name":"Center for Vaccines and Therapeutic Antibodies for Emerging Infectious Diseases, Shimane University, Izumo 690-8504, Japan"},{"name":"Department of Virology, Department of Tropical Viral Vaccine Development, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yusuke","family":"Tanahara","sequence":"additional","affiliation":[{"name":"Department of Hygienic Chemistry, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4919-7639","authenticated-orcid":false,"given":"Kodai","family":"Nishi","sequence":"additional","affiliation":[{"name":"Department of Radioisotope Medicine, Atomic Bomb Disease Institute, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5198-4711","authenticated-orcid":false,"given":"Sakura","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Department of Hygienic Chemistry, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1691-7302","authenticated-orcid":false,"given":"Kazuma","family":"Ogawa","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Analytical Sciences, Graduate School of Medical Sciences, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan"},{"name":"Institute for Frontier Science Initiative, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Morio","family":"Nakayama","sequence":"additional","affiliation":[{"name":"Department of Hygienic Chemistry, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3099-0434","authenticated-orcid":false,"given":"Daisuke","family":"Hayasaka","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Microbiology, Joint Graduate School of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8511, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,12,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1523","DOI":"10.1056\/NEJMoa1010095","article-title":"Fever with thrombocytopenia associated with a novel bunyavirus in China","volume":"364","author":"Yu","year":"2011","journal-title":"N. 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