{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T18:22:58Z","timestamp":1785522178730,"version":"3.56.0"},"reference-count":68,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,1,5]],"date-time":"2025-01-05T00:00:00Z","timestamp":1736035200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["21545010"],"award-info":[{"award-number":["21545010"]}]},{"name":"National Natural Science Foundation of China","award":["31527803"],"award-info":[{"award-number":["31527803"]}]},{"name":"National Natural Science Foundation of China","award":["2020JJ4599"],"award-info":[{"award-number":["2020JJ4599"]}]},{"name":"National Natural Science Foundation of China","award":["24A0236"],"award-info":[{"award-number":["24A0236"]}]},{"name":"National Natural Science Foundation of China","award":["24B0305"],"award-info":[{"award-number":["24B0305"]}]},{"name":"National Natural Science Foundation of China","award":["CX20230921"],"award-info":[{"award-number":["CX20230921"]}]},{"name":"Natural Science Foundation of Hunan Province, China","award":["21545010"],"award-info":[{"award-number":["21545010"]}]},{"name":"Natural Science Foundation of Hunan Province, China","award":["31527803"],"award-info":[{"award-number":["31527803"]}]},{"name":"Natural Science Foundation of Hunan Province, China","award":["2020JJ4599"],"award-info":[{"award-number":["2020JJ4599"]}]},{"name":"Natural Science Foundation of Hunan Province, China","award":["24A0236"],"award-info":[{"award-number":["24A0236"]}]},{"name":"Natural Science Foundation of Hunan Province, China","award":["24B0305"],"award-info":[{"award-number":["24B0305"]}]},{"name":"Natural Science Foundation of Hunan Province, China","award":["CX20230921"],"award-info":[{"award-number":["CX20230921"]}]},{"name":"Scientific Research Fund of Hunan Provincial Education Department of China","award":["21545010"],"award-info":[{"award-number":["21545010"]}]},{"name":"Scientific Research Fund of Hunan Provincial Education Department of China","award":["31527803"],"award-info":[{"award-number":["31527803"]}]},{"name":"Scientific Research Fund of Hunan Provincial Education Department of China","award":["2020JJ4599"],"award-info":[{"award-number":["2020JJ4599"]}]},{"name":"Scientific Research Fund of Hunan Provincial Education Department of China","award":["24A0236"],"award-info":[{"award-number":["24A0236"]}]},{"name":"Scientific Research Fund of Hunan Provincial Education Department of China","award":["24B0305"],"award-info":[{"award-number":["24B0305"]}]},{"name":"Scientific Research Fund of Hunan Provincial Education Department of China","award":["CX20230921"],"award-info":[{"award-number":["CX20230921"]}]},{"name":"Postgraduate Scientific Research Innovation Project of Hunan Province","award":["21545010"],"award-info":[{"award-number":["21545010"]}]},{"name":"Postgraduate Scientific Research Innovation Project of Hunan Province","award":["31527803"],"award-info":[{"award-number":["31527803"]}]},{"name":"Postgraduate Scientific Research Innovation Project of Hunan Province","award":["2020JJ4599"],"award-info":[{"award-number":["2020JJ4599"]}]},{"name":"Postgraduate Scientific Research Innovation Project of Hunan Province","award":["24A0236"],"award-info":[{"award-number":["24A0236"]}]},{"name":"Postgraduate Scientific Research Innovation Project of Hunan Province","award":["24B0305"],"award-info":[{"award-number":["24B0305"]}]},{"name":"Postgraduate Scientific Research Innovation Project of Hunan Province","award":["CX20230921"],"award-info":[{"award-number":["CX20230921"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Compared to natural enzymes, the development of efficient artificial simulated enzymes, such as those based on bimetallic materials with high catalytic activity and good stability, is an important way until now. Herein, we employed ZnCo2O4 microspheres as carriers to synthesize Pt-doped composites with different amounts using a one-pot method. The morphology and structure of the synthesized materials were characterized using XRD, SEM, BET, FT-IR, XPS, and Zeta potential techniques. It was found that Pt0 adhered well to the surface of ZnCo2O4 microspheres, with a 12.5% Pt doped ratio exhibiting abundant oxygen vacancies, excellent substrate affinity, and high peroxidase-like activity. Using fluorescent probes and electrochemical methods, the peroxidase-like catalytic mechanism has been explored that Pt@ZnCo2O4 microspheres can accelerate the electron transfer between H2O2 and 3,3\u2032,5,5\u2032-tetramethylbenzidine (TMB). Based on the optimal loading ratio of 12.5% of Pt@ZnCo2O4, a colorimetric sensor for visual detection of L-cysteine (L-Cys) was constructed, exhibiting a wide linear range of 0.1~50 \u00b5M and a low detection limit of 0.0163 \u00b5M. The sensor possesses good selectivity, reusability, and usage stability, which can be well applied to the determination of L-Cys in health product capsules with recovery rates of 96.9%~103.7% and RSD of 1.07%~6.50%. This work broadens the application prospects of spinel materials such as ZnCo2O4 in the field of biological analysis and also provides inspiration for the development of new artificial simulated enzymes.<\/jats:p>","DOI":"10.3390\/molecules30010187","type":"journal-article","created":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T06:43:04Z","timestamp":1736145784000},"page":"187","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Pt@ZnCo2O4 Microspheres as Peroxidase Mimics: Enhanced Catalytic Activity and Application for L-Cysteine Detection"],"prefix":"10.3390","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-5325-2537","authenticated-orcid":false,"given":"Shuqi","family":"Liao","sequence":"first","affiliation":[{"name":"Hunan Provincial Key Laboratory of Materials Protection for Electric Power and Transportation & Hunan Provincial Key Laboratory of Cytochemistry, School of Chemistry and Chemical Engineering, Changsha University of Science and Technology, Changsha 410114, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weisen","family":"Deng","sequence":"additional","affiliation":[{"name":"Hunan Provincial Key Laboratory of Materials Protection for Electric Power and Transportation & Hunan Provincial Key Laboratory of Cytochemistry, School of Chemistry and Chemical Engineering, Changsha University of Science and Technology, Changsha 410114, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Yang","sequence":"additional","affiliation":[{"name":"Hunan Provincial Key Laboratory of Materials Protection for Electric Power and Transportation & Hunan Provincial Key Laboratory of Cytochemistry, School of Chemistry and Chemical Engineering, Changsha University of Science and Technology, Changsha 410114, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jutao","family":"Zhou","sequence":"additional","affiliation":[{"name":"Hunan Provincial Key Laboratory of Materials Protection for Electric Power and Transportation & Hunan Provincial Key Laboratory of Cytochemistry, School of Chemistry and Chemical Engineering, Changsha University of Science and Technology, Changsha 410114, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Wu","sequence":"additional","affiliation":[{"name":"Hunan Provincial Key Laboratory of Materials Protection for Electric Power and Transportation & Hunan Provincial Key Laboratory of Cytochemistry, School of Chemistry and Chemical Engineering, Changsha University of Science and Technology, Changsha 410114, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3035-5035","authenticated-orcid":false,"given":"Donghong","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Bioscience, Aalborg University, DK-9220 Aalborg, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8521-2552","authenticated-orcid":false,"given":"Zhong","family":"Cao","sequence":"additional","affiliation":[{"name":"Hunan Provincial Key Laboratory of Materials Protection for Electric Power and Transportation & Hunan Provincial Key Laboratory of Cytochemistry, School of Chemistry and Chemical Engineering, Changsha University of Science and Technology, Changsha 410114, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"155354","DOI":"10.1016\/j.cej.2024.155354","article-title":"Reduced spinel oxide ZnCo2O4 with tetrahedral Co2+ sites for electrochemical nitrate reduction to ammonia and energy conversion","volume":"498","author":"Ye","year":"2024","journal-title":"Chem. 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