{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T10:17:13Z","timestamp":1785838633097,"version":"3.56.0"},"reference-count":44,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,1,21]],"date-time":"2025-01-21T00:00:00Z","timestamp":1737417600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Spanish Ministry of Economy and Competitiveness","award":["AGL2016-76699-R-AR"],"award-info":[{"award-number":["AGL2016-76699-R-AR"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Cyperus esculentus L. var sativus is cultivated in Spain, only in the L\u2019Horta Nord in the Valencia region. In this country, tubers are consumed fresh to make a popular beverage in the Valencia region called \u201chorchata de chufa\u201d (chufa milk). This drink is considered beneficial for human health thanks to its high nutritional value and medicinal importance in several treatments. This work evaluates the antifungal potential of the Thymus zygis essential oil against fungi found in tiger nut warehouses to preserve tubers under the best conditions. The analyzed commercial thyme essential oil belongs to the thymol\/p-cymene\/\u03b3-terpinene chemotype. Thymol was found in larger quantities (51.34%), followed by the identified biogenetic precursors p-cymene (35.16%) and \u03b3-terpinene (3.53%). Carvacrol also appeared, but in small quantities (3.53%). During in vitro tests, the T. zygis EO showed strong inhibition (98.85% to 91.81% MGI) against fungi Alternaria alternata, Fusarium andiyazi, Fusarium incarnatum, and Fusarium oxysporum at 300 \u00b5g\/mL. It totally inhibited their growth (100% MGI) at 400 \u00b5g\/mL, and did so strongly (75.94%, 72.02%, and 70.78%) with fungi Podospora australis, Penicillium commune, and Cladosporium subuliforme, respectively. Under in vivo conditions, formulated as a protective biofilm, and by forcing the environmental conditions of temperature and humidity to the maximum for fungus F. andiyazi growth on tiger nut tubers, the created film acted as a strong protector against fungal attacks.<\/jats:p>","DOI":"10.3390\/molecules30030436","type":"journal-article","created":{"date-parts":[[2025,1,21]],"date-time":"2025-01-21T04:19:51Z","timestamp":1737433191000},"page":"436","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Conservation of Tiger Nut Tubers with Natural Biofilm Formulated with Thymus zygis Essential Oil"],"prefix":"10.3390","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2461-9794","authenticated-orcid":false,"given":"M. Pilar","family":"Santamarina","sequence":"first","affiliation":[{"name":"Departamento de Ecosistemas Agroforestales, Universitat Polit\u00e8cnica de Val\u00e8ncia, Camino de Vera s\/n, 46022 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8021-4348","authenticated-orcid":false,"given":"Silvia","family":"Gim\u00e9nez-Santamarina","sequence":"additional","affiliation":[{"name":"Departamento de Ecosistemas Agroforestales, Universitat Polit\u00e8cnica de Val\u00e8ncia, Camino de Vera s\/n, 46022 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Santamarina","sequence":"additional","affiliation":[{"name":"Departamento Bayer Crop Science, Escuela T\u00e9cnica Superior de Ingenier\u00eda Agron\u00f3mica y del Medio Natural (ETSIAMN), Universitat Polit\u00e8cnica de Val\u00e8ncia, Camino de Vera s\/n, 46022 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvina","family":"Larran","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones de Fitopatolog\u00eda (CIDEFI), Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, La Plata 1900, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josefa","family":"Rosell\u00f3","sequence":"additional","affiliation":[{"name":"Departamento de Ecosistemas Agroforestales, Universitat Polit\u00e8cnica de Val\u00e8ncia, Camino de Vera s\/n, 46022 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.sajb.2021.08.010","article-title":"Traditional uses, phytochemistry, and anticancer potential of Cyperus rotundus L. (Cyperaceae): A systematic review","volume":"144","year":"2022","journal-title":"S. Afr. J. Bot."},{"key":"ref_2","first-page":"8","article-title":"Cyperus L.","volume":"Volume XVIII","author":"Castroviejo","year":"2007","journal-title":"Flora Ib\u00e9rica. Plantas vasculares de la Pen\u00ednsula Ib\u00e9rica e Islas Baleares"},{"key":"ref_3","unstructured":"Serrallach, J. (1927). Die wurzelknolle von Cyperus esculentus L.. [Ph.D. Thesis, University Frankfurt am Main]."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.ppees.2016.09.003","article-title":"Biological flora of Central Europe: Cyperus esculentus L.","volume":"23","author":"Follak","year":"2016","journal-title":"Perspect. Plant Ecol. Evol. Syst."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Zhang, S., Li, P., Wei, Z., Cheng, Y., Liu, J., Yang, Y., Wang, Y., and Mu, Z. (2022). Cyperus (Cyperus esculentus L.): A Review of Its Compositions, Medical Efficacy, Antibacterial Activity and Allelopathic Potentials. Plants, 11.","DOI":"10.3390\/plants11091127"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Marticorena, C., and Marchesi, E. (2008). Cat\u00e1logo de las Plantas Vasculares del Cono Sur (Argentina, Sur de Brasil, Chile, Paraguay y Uruguay), Missouri Botanical Garden Press.","DOI":"10.4067\/S0716-078X2009000400012"},{"key":"ref_7","first-page":"287","article-title":"Die Cyperaceen des k\u00f6niglichen Herbariums zu Berlin","volume":"36","author":"Boeckeler","year":"1870","journal-title":"Linnaea"},{"key":"ref_8","first-page":"178","article-title":"On the Indian species of Cyperus","volume":"21","author":"Clarke","year":"1884","journal-title":"J. Linn. Soc., Bot."},{"key":"ref_9","first-page":"1","article-title":"The sedges of Jamaica (Cyperaceae)","volume":"5","author":"Britton","year":"1907","journal-title":"Bull. Dept. Agric. Jamaica"},{"key":"ref_10","first-page":"101","article-title":"Cyperaceae\u2013Scirpoideae\u2013Cypereae","volume":"Volume 4","author":"Engler","year":"1936","journal-title":"Das Pflanzenreich. Regni Vegetabilis Conspectus"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1614\/P2002-131","article-title":"Genetic and morphological analysis of two novel nutsedge biotypes from California","volume":"51","author":"Tayyar","year":"2003","journal-title":"Weed Sci."},{"key":"ref_12","first-page":"192","article-title":"Characteristics and Research Progress of Cyperus esculentus L.","volume":"17","author":"Yang","year":"2017","journal-title":"North. Hortic."},{"key":"ref_13","first-page":"219","article-title":"Cyperus Linnaeus","volume":"Volume 23","author":"Wu","year":"2010","journal-title":"Flora of China"},{"key":"ref_14","first-page":"1174","article-title":"Research Progress on main nutritional components and physiological functions of tiger nut (Cyperus esculentus L.)","volume":"43","author":"Guo","year":"2021","journal-title":"Chin. J. Oil Crop Sci."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Zheng, X., Liu, J., Cheng, Z., Sun, Y., Li, L., and Wang, J. (2024). Improving Tuber Yield of Tiger Nut (Cyperus esculentus L.) through Nitrogen Fertilization in Sandy Farmland. Plants, 13.","DOI":"10.3390\/plants13081063"},{"key":"ref_16","unstructured":"(2024, December 10). Consejo Regulador Denominaci\u00f3n de Origen Chufa de Valencia. Available online: https:\/\/chufadevalencia.org\/la-chufa\/."},{"key":"ref_17","first-page":"627","article-title":"Sintomatolog\u00eda y control de la necrosis foliar de la chufa (Cyperus esculentus L.)","volume":"3","year":"2012","journal-title":"Revista Cient\u00edfica UDO Agr\u00edcola"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"386","DOI":"10.21273\/HORTSCI.48.3.386","article-title":"\u2018Alboraia\u2019 and \u2018Bonrepos\u2019: The First Registered Chufa (Cyperus esculentus L. var. sativus Boeck.) Cultivars","volume":"48","author":"Maroto","year":"2013","journal-title":"HortScience"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Hollowell, J.E., and Shew, B.B. (2001). Yellow nutsedge (Cyperus esculentus L.) as a host of Sclerotinia minor. Plant Dis., 85.","DOI":"10.1094\/PDIS.2001.85.5.562C"},{"key":"ref_20","first-page":"148","article-title":"Fungi for biological control of weedy Cyperaceae, with emphasis on purple and yellow nutsedges (Cyperus rotundus and C. esculentus)","volume":"14","author":"Charudattan","year":"2005","journal-title":"Outlooks Pest Manag."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1080\/00275514.1988.12025561","article-title":"Cercospora caricis from Cyperus esculentus (yellow nutsedge): Morphology and cercosporin production","volume":"80","author":"Blaney","year":"1988","journal-title":"Mycologia"},{"key":"ref_22","first-page":"144","article-title":"La podredumbre negra o \u201calquitranat\u201d: Un grave problema de la chufa en Valencia","volume":"183","author":"Busto","year":"1997","journal-title":"Agr\u00edcola Vergel"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1281","DOI":"10.1094\/PDIS.1998.82.11.1281A","article-title":"A tuber rot of Cyperus esculentus caused by Rosellinia necatrix","volume":"82","author":"Busto","year":"1998","journal-title":"Plant Dis."},{"key":"ref_24","first-page":"50","article-title":"Identificaci\u00f3n de un virus como el causante de una nueva enfermedad que afecta al cultivo de chufa en Valencia","volume":"344","author":"Rubio","year":"2022","journal-title":"Phytoma"},{"key":"ref_25","first-page":"205","article-title":"Mejora de la sanidad y calidad de la chufa en valencia: Situaci\u00f3n actual","volume":"402","author":"Marsal","year":"2017","journal-title":"Agr\u00edcola Vergel Frutic. Hortic. Floric."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.crfs.2019.11.005","article-title":"Cherry tomato and persimmon kaki conservation with a natural and biodegradable film","volume":"2","author":"Verdeguer","year":"2020","journal-title":"Curr. Res. Food Sci."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Allagui, M.B., Moumni, M., and Romanazzi, G. (2024). Antifungal activity of thirty essential oils to control pathogenic fungi of postharvest decay. Antibiotics, 13.","DOI":"10.3390\/antibiotics13010028"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Ji, F., He, D., Olaniran, A.O., Mokoena, M.P., Xu, J., and Shi, J. (2019). Occurrence, toxicity, production and detection of Fusarium mycotoxin: A review. Food Prod. Process. Nutr., 1.","DOI":"10.1186\/s43014-019-0007-2"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Ekwomadu, T.I., Akinola, S.A., and Mwanza, M. (2021). Fusarium mycotoxins, their metabolites (free, emerging, and masked), food safety concerns, and health impacts. Int. J. Environ. Res. Public Health, 18.","DOI":"10.3390\/ijerph182211741"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Alonso, P., Fern\u00e1ndez-Pastor, S., and Guerrero, A. (2024). Application of Cinnamon Essential Oil in Active Food Packaging: A Review. Appl. Sci., 14.","DOI":"10.3390\/app14156554"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Gan, N., Song, Y., Li, Y., Liu, P., Chen, S., He, Y., Zeng, T., Wang, W., and Wu, D. (2025). Characterization of the effects of bridging linker on the \u03b2-Lactoglobulin binding mechanism on the nanoscale metal-organic framework. Food Chemistry, 464 Pt 2.","DOI":"10.1016\/j.foodchem.2024.141715"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1669","DOI":"10.1016\/S0278-6915(02)00120-5","article-title":"Effect of oil extracted from some medicinal plants on different mycotoxigenic fungi","volume":"40","author":"Soliman","year":"2002","journal-title":"Food Chem. Toxicol."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Rosell\u00f3, J., Llorens-Molina, J.A., Larran, S., Sempere-Ferre, F., and Santamarina, M.P. (2024). Biofilm containing the Thymus serpyllum essential oil for rice and cherry tomato conservation. Front. Plant Sci., 15.","DOI":"10.3389\/fpls.2024.1362569"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1218","DOI":"10.4315\/0362-028X.JFP-12-554","article-title":"In vitro antioxidant and antifungal properties of essential oils obtained from aromatic herbs endemic to the southeast of Spain","volume":"76","author":"Sendra","year":"2013","journal-title":"J. Food Prot."},{"key":"ref_35","unstructured":"Adams, R.P. (2007). Identification of Essential Components by Gas Chromatography\/Mass Spectrometry, Allured Publishing Corporation. [4th ed.]."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1016\/j.foodcont.2011.11.004","article-title":"Occurrence of fourteen mycotoxins in tiger-nuts","volume":"25","author":"Rubert","year":"2012","journal-title":"Food Control"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.jfutfo.2022.03.002","article-title":"An overview on the major mycotoxins in food products: Characteristics, toxicity, and analysis","volume":"2","author":"Jebur","year":"2022","journal-title":"J. Future Foods"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Sempere-Ferre, F., Asamar, J., Castell, V., Rosell\u00f3, J., and Santamarina, M.P. (2021). Evaluating the antifungal potential of botanical compounds to control Botryotinia fuckeliana and Rhizoctonia solani. Molecules, 26.","DOI":"10.3390\/molecules26092472"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1111\/j.1472-765X.2006.02032.x","article-title":"Antifungal activity of thyme (Thymus vulgaris L.) essential oil and thymol against moulds from damp dwellings","volume":"44","author":"Kosalec","year":"2007","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1016\/j.foodcont.2018.05.004","article-title":"Antifungal and functional properties of starch-gellan films containing thyme (Thymus zygis) essential oil","volume":"92","author":"Sapper","year":"2018","journal-title":"Food Control"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Sapper, M., Palou, L., P\u00e9rez-Gago, M.B., and Chiralt, A. (2019). Antifungal starch\u2013gellan edible coatings with Thyme essential oil for the postharvest preservation of apple and persimmon. Coatings, 9.","DOI":"10.3390\/coatings9050333"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"6241","DOI":"10.1073\/pnas.1117018109","article-title":"Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for fungi","volume":"109","author":"Schoch","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1080\/26895293.2021.2022007","article-title":"Chemical composition of essential oils of three Mentha species and their antifungal activity against selected phytopathogenic and post-harvest fungi","volume":"15","author":"Santamarina","year":"2022","journal-title":"All Life"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1590\/S1516-89132006000500001","article-title":"de L.R.; Willadino, L.; Marcelino Junior, C.; Ulisses, C. Antimicrobial action of the essential oil of Lippia gracilis Schauer","volume":"49","author":"Albuquerque","year":"2006","journal-title":"Braz. Arch. Biol. Technol."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/30\/3\/436\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T10:32:35Z","timestamp":1759919555000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/30\/3\/436"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,21]]},"references-count":44,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2025,2]]}},"alternative-id":["molecules30030436"],"URL":"https:\/\/doi.org\/10.3390\/molecules30030436","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,21]]}}}