{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T22:08:12Z","timestamp":1781647692978,"version":"3.54.5"},"reference-count":43,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2025,2,14]],"date-time":"2025-02-14T00:00:00Z","timestamp":1739491200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NIH","award":["R21AI151483"],"award-info":[{"award-number":["R21AI151483"]}]},{"name":"NIH","award":["HHSN272201800013C"],"award-info":[{"award-number":["HHSN272201800013C"]}]},{"name":"Laulima Government Solutions, LLC","award":["R21AI151483"],"award-info":[{"award-number":["R21AI151483"]}]},{"name":"Laulima Government Solutions, LLC","award":["HHSN272201800013C"],"award-info":[{"award-number":["HHSN272201800013C"]}]},{"name":"NIAID Division of Microbiology and Infectious Diseases (MRH)","award":["R21AI151483"],"award-info":[{"award-number":["R21AI151483"]}]},{"name":"NIAID Division of Microbiology and Infectious Diseases (MRH)","award":["HHSN272201800013C"],"award-info":[{"award-number":["HHSN272201800013C"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Algal lectin Griffithsin (GRFT) is a well-known mannose-binding protein which has broad-spectrum antiviral activity against several important infectious viruses including HIV, HCV, and SARS-CoV-2. Therefore, GRFT has been brought great attention to antiviral therapeutic development. In this report, we have tested GRFT\u2019s activity against the lethal Ebola virus in vitro and in vivo. Our data have shown that the IC50 value is about 42 nM for inhibiting Zaire Ebola virus (EBOV) infection in vitro. The preliminary in vivo mice model using mouse-adapted EBOV has also shown a certain efficacy for delayed mortality compared to the control animals. A GRFT pull-down experiment using viral particles demonstrates that GRFT can bind to N-glycans of EBOV. Thus, it can be concluded that GRFT, through binding to viral glycans, may block Ebola virus infection and has potential for the treatment of Ebola virus disease (EVD).<\/jats:p>","DOI":"10.3390\/molecules30040892","type":"journal-article","created":{"date-parts":[[2025,2,17]],"date-time":"2025-02-17T12:36:57Z","timestamp":1739795817000},"page":"892","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Algal Lectin Griffithsin Inhibits Ebola Virus Infection"],"prefix":"10.3390","volume":"30","author":[{"given":"Leah Liu","family":"Wang","sequence":"first","affiliation":[{"name":"Nebraska Center for Virology, School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE 68588, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6031-5360","authenticated-orcid":false,"given":"Kendra","family":"Alfson","sequence":"additional","affiliation":[{"name":"Texas Biomedical Research Institute, 8715 W. Military Drive, San Antonio, TX 78227, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brett","family":"Eaton","sequence":"additional","affiliation":[{"name":"Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, Frederick, MD 21702, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc E.","family":"Mattix","sequence":"additional","affiliation":[{"name":"Nonclinical Pathology Services, LLC, 5920 Clubhouse Pointe Dr., Medina 44256, OH, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yenny","family":"Goez-Gazi","sequence":"additional","affiliation":[{"name":"Texas Biomedical Research Institute, 8715 W. Military Drive, San Antonio, TX 78227, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0824-2667","authenticated-orcid":false,"given":"Michael R.","family":"Holbrook","sequence":"additional","affiliation":[{"name":"Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, Frederick, MD 21702, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Ricardo","family":"Carrion","sequence":"additional","affiliation":[{"name":"Texas Biomedical Research Institute, 8715 W. Military Drive, San Antonio, TX 78227, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5656-5698","authenticated-orcid":false,"given":"Shi-Hua","family":"Xiang","sequence":"additional","affiliation":[{"name":"Nebraska Center for Virology, School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE 68588, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"9345","DOI":"10.1074\/jbc.M411122200","article-title":"Isolation and characterization of griffithsin, a novel HIV-inactivating protein, from the red alga Griffithsia sp","volume":"280","author":"Mori","year":"2005","journal-title":"J. Biol. Chem."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Lee, C. (2019). 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