{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T07:11:07Z","timestamp":1788246667504,"version":"build-2803163510"},"reference-count":112,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2025,2,18]],"date-time":"2025-02-18T00:00:00Z","timestamp":1739836800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004901","name":"FAPEMIG (Foundation of Support Research of the State of Minas Gerais)","doi-asserted-by":"publisher","award":["APQ 03057-22"],"award-info":[{"award-number":["APQ 03057-22"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Diplopterys pubipetala (Malpighiaceae) is a liana native to the Brazilian Cerrado biome, traditionally used in Ayahuasca preparations. Despite its cultural importance, research on its chemical composition and biological activities, which may have therapeutic potential, is limited. This study investigated the volatile and non-volatile secondary metabolites of D. pubipetala leaves, their antioxidant capacity, and their antibacterial and antifungal activities. Volatile compounds were identified using gas chromatography-mass spectrometry (GC-MS) coupled to solid-phase microextraction (SPME), while non-volatile compounds were annotated using UHPLC-MS\/MS-ESI-Q-TOF. Antioxidant capacity was evaluated by DPPH assay, and antimicrobial activity was assessed in vitro against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Candida species (C. albicans, C. tropicalis, C. glabrata). GC-MS analysis revealed 25 predominant volatile compounds, including ethyl dodecanoate, ethyl tetradecanoate, nonanoic acid, and 5-methylhexan-2-one, with documented antifungal, antioxidant, and antimicrobial activities. The crude extract and ethyl acetate fraction showed strong antioxidant capacity (EC50 9.83 \u00b5g\/mL and 6.42 \u00b5g\/mL, respectively), and antifungal effects were observed against Candida species. This study provides the first comprehensive investigation of the antioxidant capacity and antibacterial and antifungal activities of D. pubipetala, together with a detailed chemical profile of its volatile compounds.<\/jats:p>","DOI":"10.3390\/molecules30040946","type":"journal-article","created":{"date-parts":[[2025,2,18]],"date-time":"2025-02-18T08:36:19Z","timestamp":1739867779000},"page":"946","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Diplopterys pubipetala (Malpighiaceae): Insights into Antioxidant, Antibacterial, and Antifungal Activities with Chemical Composition Analysis via UHPLC-MS\/MS and GC\/MS"],"prefix":"10.3390","volume":"30","author":[{"given":"Veronica","family":"de Melo Sacramento","sequence":"first","affiliation":[{"name":"Natural Products Laboratory, State University of Montes Claros, Montes Claros 39401-089, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4842-3569","authenticated-orcid":false,"given":"Vanessa","family":"de Andrade Royo","sequence":"additional","affiliation":[{"name":"Natural Products Laboratory, State University of Montes Claros, Montes Claros 39401-089, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2802-1244","authenticated-orcid":false,"given":"Pedro Henrique","family":"Fonseca Veloso","sequence":"additional","affiliation":[{"name":"Natural Products Laboratory, State University of Montes Claros, Montes Claros 39401-089, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-1084-1679","authenticated-orcid":false,"given":"Kamila Soares","family":"Freitas Souto","sequence":"additional","affiliation":[{"name":"Natural Products Laboratory, State University of Montes Claros, Montes Claros 39401-089, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0998-1939","authenticated-orcid":false,"given":"Alisson Samuel","family":"Portes Caldeira","sequence":"additional","affiliation":[{"name":"Laboratory of Bioactive Natural Products Chemistry, Ren\u00e9 Rachou Institute, Oswaldo Cruz Foundation, Belo Horizonte 30190-002, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8634-6878","authenticated-orcid":false,"given":"Carlos Henrique","family":"Gomes Martins","sequence":"additional","affiliation":[{"name":"Antimicrobial Testing Laboratory, Institute of Biomedical Sciences, Federal University of Uberl\u00e2ndia, Uberl\u00e2ndia 38405-320, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara Lemes","family":"de Souza","sequence":"additional","affiliation":[{"name":"Antimicrobial Testing Laboratory, Institute of Biomedical Sciences, Federal University of 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MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dario Alves de","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Bioprospecting and Genetic Resources Laboratory, State University of Montes Claros, Montes Claros 39401-089, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elytania Veiga","family":"Mnezes","sequence":"additional","affiliation":[{"name":"Bioprospecting and Genetic Resources Laboratory, State University of Montes Claros, Montes Claros 39401-089, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0582-3968","authenticated-orcid":false,"given":"T\u00e2nia Maria de Almeida","family":"Alves","sequence":"additional","affiliation":[{"name":"Laboratory of Bioactive Natural Products Chemistry, Ren\u00e9 Rachou Institute, Oswaldo Cruz Foundation, Belo Horizonte 30190-002, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1590\/S0100-40422002000300016","article-title":"Plantas medicinais: A necessidade de estudos multidisciplinares","volume":"2","author":"Maciel","year":"2002","journal-title":"Qu\u00edm. 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