{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T00:00:12Z","timestamp":1790899212564,"version":"4.1.0"},"reference-count":37,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T00:00:00Z","timestamp":1739923200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Regional Development Fund","award":["810630"],"award-info":[{"award-number":["810630"]}]},{"name":"European Regional Development Fund","award":["2021-2027.5.02.23-0095"],"award-info":[{"award-number":["2021-2027.5.02.23-0095"]}]},{"name":"European Union\u2019s Horizon 2020 Research and Innovation Program","award":["810630"],"award-info":[{"award-number":["810630"]}]},{"name":"European Union\u2019s Horizon 2020 Research and Innovation Program","award":["2021-2027.5.02.23-0095"],"award-info":[{"award-number":["2021-2027.5.02.23-0095"]}]},{"name":"European Regional Development Fund (ERDF)","award":["810630"],"award-info":[{"award-number":["810630"]}]},{"name":"European Regional Development Fund (ERDF)","award":["2021-2027.5.02.23-0095"],"award-info":[{"award-number":["2021-2027.5.02.23-0095"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Fruit processing by-products contain various classes of bioactive constituents, which may find applications as ingredients for foods, nutraceuticals or cosmeceuticals. This study explored the fractionation of lipophilic rowanberry pomace extracts isolated with pure supercritical CO2 and its mixtures with a co-solvent ethanol by their on-line separation at subcritical conditions. Rowanberry pomace lipids were extracted with supercritical CO2 (42.4 MPa, 53 \u00b0C) using 0\u20137% of ethanol, and then fractionated by reducing the first separator\u2019s (S1) pressure to 7 MPa and cooling it to 0, \u221210 and \u221220 \u00b0C to precipitate the \u2018heavier\u2019 fraction (HF). The second separator (S2) was depressurized at ambient temperatures to collect the \u2018lighter\u2019 fraction (LF). The yield of the LF increased by decreasing the S1 temperature and increasing the amount of the co-solvent. The concentration of \u03b2-carotene was increased in the LF by decreasing the S1 temperature and increasing the co-solvent concentration; at \u221220 \u00b0C it was 66.7% higher than in the non-fractionated extract. The concentrations of tocopherols and phytosterols were also remarkably higher in the LF. In total, 62 compounds were identified in the headspace volatile fraction of the LF, benzaldehyde and benzyl alcohol being the most abundant constituents. In conclusion, fractionation enabled us to obtain fractions with higher concentrations of the selected classes of lipophilic rowanberry constituents.<\/jats:p>","DOI":"10.3390\/molecules30040964","type":"journal-article","created":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T08:36:46Z","timestamp":1739954206000},"page":"964","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Supercritical Fluid Extraction of Lipids from Rowanberry Pomace with Pure CO2 and Its Mixtures with Ethanol Followed by the On-Line Separation of Fractions"],"prefix":"10.3390","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6241-8475","authenticated-orcid":false,"given":"Viive","family":"Sarv","sequence":"first","affiliation":[{"name":"Polli Horticultural Research Centre, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, 69108 Viljandi, Estonia"},{"name":"ERA-Chair for Food (By-) Products Valorisation Technologies (VALORTECH), Estonian University of Life Sciences, 51006 Tartu, Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3391-6624","authenticated-orcid":false,"given":"Rajeev","family":"Bhat","sequence":"additional","affiliation":[{"name":"ERA-Chair for Food (By-) Products Valorisation Technologies (VALORTECH), Estonian University of Life Sciences, 51006 Tartu, Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"J\u016brien\u0117","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology, Kaunas University of Technology, LT-50254 Kaunas, Lithuania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renata","family":"Baranauskien\u0117","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology, Kaunas University of Technology, LT-50254 Kaunas, Lithuania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2913-2739","authenticated-orcid":false,"given":"Dalia","family":"Urbonavi\u010dien\u0117","sequence":"additional","affiliation":[{"name":"Biochemistry and Technology Laboratory, Institute of Horticulture LAMMC, LT-54333 Kaunas, Lithuania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5546-0168","authenticated-orcid":false,"given":"Pranas","family":"Vi\u0161kelis","sequence":"additional","affiliation":[{"name":"Biochemistry and Technology Laboratory, Institute of Horticulture LAMMC, LT-54333 Kaunas, Lithuania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6063-8132","authenticated-orcid":false,"given":"Petras Rimantas","family":"Venskutonis","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology, Kaunas University of Technology, LT-50254 Kaunas, Lithuania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,19]]},"reference":[{"key":"ref_1","unstructured":"European Commission (2020). 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