{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T13:53:54Z","timestamp":1761746034076,"version":"build-2065373602"},"reference-count":34,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T00:00:00Z","timestamp":1740096000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Education, Science and Technological development of the Republic of Serbia","award":["451-03-47\/2023-01\/200019"],"award-info":[{"award-number":["451-03-47\/2023-01\/200019"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>A fast and simple biotech method is presented for the simultaneous isolation and purification of transferrin (Tf) and immunoglobulin G (IgG) from the same pool-sample of human serum, yielding &gt;98% pure proteins. Serum sample preparation was achieved by precipitation with ethacridine lactate (rivanol). Protein purification was performed with AKTA Avant 150 FPLC, using a Resource Q column. Three different buffers at pH 6.2 (MES, phosphate, and Bis-Tris) were tested. Isolated and purified proteins retained their native 3D structure, as shown by spectrofluorimetric measurements. Tf functionality was preserved, as confirmed by the retention of both the iron binding capacity and its ability to interact with the transferrin receptor (immunofluorescent staining), as well as the immunogenicity of IgG, as shown by Western blot analysis with immunodetection. The formation of IgG aggregates was avoided. This biotech method is a rapid, simple, and time-saving alternative to other methods for the isolation of extremely pure IgG and Tf, while it is also the only method so far described for their simultaneous isolation.<\/jats:p>","DOI":"10.3390\/molecules30050993","type":"journal-article","created":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T07:53:06Z","timestamp":1740124386000},"page":"993","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Simultaneous Isolation and Purification of Transferrin and Immunoglobulin G from Human Serum\u2014A New Biotech Solution"],"prefix":"10.3390","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9705-3237","authenticated-orcid":false,"given":"Danilo","family":"\u010ceti\u0107","sequence":"first","affiliation":[{"name":"Institute for the Application of Nuclear Energy INEP, University of Belgrade, Banatska 31 b, 11000 Belgrade, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Goran","family":"Milju\u0161","sequence":"additional","affiliation":[{"name":"Institute for the Application of Nuclear Energy INEP, University of Belgrade, Banatska 31 b, 11000 Belgrade, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4652-7719","authenticated-orcid":false,"given":"Zorana","family":"Dobrijevi\u0107","sequence":"additional","affiliation":[{"name":"Institute for the Application of Nuclear Energy INEP, University of Belgrade, Banatska 31 b, 11000 Belgrade, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8691-2486","authenticated-orcid":false,"given":"Nikola","family":"Gligorijevi\u0107","sequence":"additional","affiliation":[{"name":"Institute for the Application of Nuclear Energy INEP, University of Belgrade, Banatska 31 b, 11000 Belgrade, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aleksandra","family":"Viloti\u0107","sequence":"additional","affiliation":[{"name":"Institute for the Application of Nuclear Energy INEP, University of Belgrade, Banatska 31 b, 11000 Belgrade, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2042-0056","authenticated-orcid":false,"given":"Olgica","family":"Nedi\u0107","sequence":"additional","affiliation":[{"name":"Institute for the Application of Nuclear Energy INEP, University of Belgrade, Banatska 31 b, 11000 Belgrade, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2539-4507","authenticated-orcid":false,"given":"Ana","family":"Penezi\u0107","sequence":"additional","affiliation":[{"name":"Institute for the Application of Nuclear Energy INEP, University of Belgrade, Banatska 31 b, 11000 Belgrade, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.freeradbiomed.2018.06.037","article-title":"Transferrin and transferrin receptors update","volume":"133","author":"Kawabata","year":"2019","journal-title":"Free Radic. Biol. Med."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"214186","DOI":"10.1016\/j.ccr.2021.214186","article-title":"Human transferrin: An inorganic biochemistry perspective","volume":"449","author":"Silva","year":"2021","journal-title":"Coord. Chem. Rev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0009-8981(90)90278-Z","article-title":"The biology of transferrin","volume":"190","year":"1990","journal-title":"Clin. Chim. Acta"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"7317","DOI":"10.1073\/pnas.1914830117","article-title":"Iron sequestration by transferrin 1 mediates nutritional immunity in Drosophila melanogaster","volume":"117","author":"Iatsenko","year":"2020","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1016\/0014-4827(89)90241-3","article-title":"Growth-Stimulating Effect of Transferrin on a Hybridoma Cell Line: Relation to Transferrin Iron-Transporting Function","volume":"182","author":"Kovar","year":"1989","journal-title":"Exp. Cell Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1124\/pr.54.4.561","article-title":"Targeted Drug Delivery via the Transferrin Receptor-Mediated Endocytosis Pathway","volume":"54","author":"Qian","year":"2002","journal-title":"Pharmacol. Rev."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1016\/j.blre.2017.07.005","article-title":"Therapeutic use of transferrin to modulate anemia and conditions of iron toxicity","volume":"31","author":"Boshuizen","year":"2017","journal-title":"Blood Rev."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/j.bbagen.2011.07.016","article-title":"The transferrin receptor and the targeted delivery of therapeutic agents against cancer","volume":"1820","author":"Daniels","year":"2012","journal-title":"Biochim. Biophys. Acta Gen. Subj."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1016\/j.str.2004.02.011","article-title":"Structure and flexibility of individual immunoglobulin G molecules in solution","volume":"12","author":"Sandin","year":"2004","journal-title":"Structure"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"121904","DOI":"10.1063\/1.4811345","article-title":"Phase transitions in human IgG solutions","volume":"139","author":"Wang","year":"2013","journal-title":"J. Chem. Phys."},{"key":"ref_11","unstructured":"Janeway, C.A., Travers, P., Walport, M., and Shlomchik, M.J. (2023, October 16). The distribution and functions of immunoglobulin isotypes, Immunobiology: The Immune System in Health and Disease, Available online: https:\/\/www.ncbi.nlm.nih.gov\/books\/NBK27162\/."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1084\/jem.20061788","article-title":"The antiinflammatory activity of IgG: The intravenous IgG paradox","volume":"204","author":"Nimmerjahn","year":"2007","journal-title":"J. Exp. Med."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1128\/CDLI.8.5.859-863.2001","article-title":"Use of intravenous immunoglobulins for prophylaxis or treatment of infectious diseases","volume":"8","author":"Hemming","year":"2001","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1016\/j.tips.2013.05.009","article-title":"Polyclonal immunoglobulin G for autoimmune demyelinating nervous system disorders","volume":"34","author":"Buttmann","year":"2013","journal-title":"Trends Pharmacol. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"910","DOI":"10.1016\/j.chroma.2008.12.004","article-title":"Purification of human immunoglobulin G via Fc-specific small peptide ligand affinity chromatography","volume":"1216","author":"Yang","year":"2009","journal-title":"J. Chromatogr. A"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/S0378-4347(98)00470-8","article-title":"Purification of immunoglobulins G by protein A\/G affinity membrane chromatography","volume":"723","author":"Dancette","year":"1999","journal-title":"J. Chromatogr. B Biomed. Sci. Appl."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Walker, J.M. (2002). IgG Purification with Ion-Exchange HPLC. The Protein Protocols Handbook, Humana Press Inc.. [2nd ed.].","DOI":"10.1385\/1592591698"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1093\/glycob\/cwg016","article-title":"The effects of ethanol on the glycosylation of human transferrin","volume":"13","author":"Flahaut","year":"2003","journal-title":"Glycobiology"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1042\/BA20100156","article-title":"A simple method for large-scale purification of plasma-derived apo-transferrin","volume":"57","author":"Ascione","year":"2010","journal-title":"Biotechnol. Appl. Biochem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1042\/BA20050065","article-title":"Purification of transferrin from Cohn supernatant I using ion-exchange chromatography","volume":"42","author":"McCann","year":"2005","journal-title":"Biotechnol. Appl. Biochem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.cccn.2005.01.021","article-title":"HPLC analysis of carbohydrate deficient transferrin isoforms isolated by the Axis-Shield %CDT method","volume":"356","author":"Ohlson","year":"2005","journal-title":"Clin. Chim. Acta"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2087","DOI":"10.1016\/j.jchromb.2010.06.009","article-title":"A new process of IgG purification by negative chromatography: Adsorption aspects of human serum proteins onto \u03c9-aminodecyl-agarose","volume":"878","author":"Bresolin","year":"2010","journal-title":"J. Chromatogr. B Analyt Technol. Biomed. Life Sci."},{"key":"ref_23","unstructured":"Page, M., and Thorpe, R. (2002). DEAE-Sepharose Chromatography Purification of IgG Using DEAE-Sepharose Chromatography. The Protein Protocols Handbook, Springer."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1260\/0263-6174.30.8-9.701","article-title":"Dye Ligand Epoxide Chitosan\/Alginate: A Potential New Stationary Phase for Human IgG Purification","volume":"30","author":"Gondim","year":"2012","journal-title":"Adsorpt. Sci. Technol."},{"key":"ref_25","first-page":"34","article-title":"A Large-Scale Method for the Purification of Human Transferrin","volume":"6","author":"Inman","year":"1961","journal-title":"Vox Sang."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1016\/0003-9861(83)90306-5","article-title":"DEAE-Affi-Gel Blue Chromatography of Human Serum: Use for Purification of Native Transferrin","volume":"226","author":"Werner","year":"1983","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.cca.2017.05.013","article-title":"A microscale protocol for the isolation of transferrin directly from serum","volume":"471","year":"2017","journal-title":"Clin. Chim. Acta"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1021\/bp030070k","article-title":"Antibodies and genetically engineered related molecules: Production and purification","volume":"20","author":"Roque","year":"2004","journal-title":"Biotechnol. Prog."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/0304-4165(94)00187-3","article-title":"Isoelectric restriction of human immunoglobulin isotypes","volume":"1243","author":"Prin","year":"1995","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1111\/j.0954-6820.1956.tb14351.x","article-title":"The Isolation of Gamma Globulin from Blood-Serum by Rivanol","volume":"155","author":"Horejsi","year":"1956","journal-title":"Acta Med. Scand."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/0304-4165(65)90407-1","article-title":"Adsorption of rivanol by potato starch in the isolation of transferrins","volume":"107","author":"Sutton","year":"1965","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_32","unstructured":"Yi, Y., and Zang, L. (2018). Factors Influencing Biotherapeutic Monoclonal Antibody Aggregation, American Pharmaceutical Review."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1559","DOI":"10.1007\/s00436-019-06257-2","article-title":"Susceptibility of human villous (BeWo) and extravillous (HTR-8\/SVneo) trophoblast cells to Toxoplasma gondii infection is modulated by intracellular iron availability","volume":"118","author":"Almeida","year":"2019","journal-title":"Parasitol. Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2703","DOI":"10.1002\/jps.20461","article-title":"Solution behavior of a novel type 1 interferon, interferon-\u03c4","volume":"94","author":"Katayama","year":"2005","journal-title":"J. Pharm. Sci."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/30\/5\/993\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T16:39:31Z","timestamp":1760027971000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/30\/5\/993"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,21]]},"references-count":34,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2025,3]]}},"alternative-id":["molecules30050993"],"URL":"https:\/\/doi.org\/10.3390\/molecules30050993","relation":{},"ISSN":["1420-3049"],"issn-type":[{"type":"electronic","value":"1420-3049"}],"subject":[],"published":{"date-parts":[[2025,2,21]]}}}