{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T22:31:53Z","timestamp":1790980313925,"version":"4.1.0"},"reference-count":46,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2025,2,24]],"date-time":"2025-02-24T00:00:00Z","timestamp":1740355200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University of Buenos Aires","award":["UBACYT 20020220300118BA"],"award-info":[{"award-number":["UBACYT 20020220300118BA"]}]},{"name":"University of Buenos Aires","award":["PICT 2020 SERIEA-03061"],"award-info":[{"award-number":["PICT 2020 SERIEA-03061"]}]},{"name":"University of Buenos Aires","award":["PICT 2019 No. 3367"],"award-info":[{"award-number":["PICT 2019 No. 3367"]}]},{"name":"University of Buenos Aires","award":["P123RT0138"],"award-info":[{"award-number":["P123RT0138"]}]},{"name":"National Agency for Science and Technology Promotion","award":["UBACYT 20020220300118BA"],"award-info":[{"award-number":["UBACYT 20020220300118BA"]}]},{"name":"National Agency for Science and Technology Promotion","award":["PICT 2020 SERIEA-03061"],"award-info":[{"award-number":["PICT 2020 SERIEA-03061"]}]},{"name":"National Agency for Science and Technology Promotion","award":["PICT 2019 No. 3367"],"award-info":[{"award-number":["PICT 2019 No. 3367"]}]},{"name":"National Agency for Science and Technology Promotion","award":["P123RT0138"],"award-info":[{"award-number":["P123RT0138"]}]},{"name":"CONICET","award":["UBACYT 20020220300118BA"],"award-info":[{"award-number":["UBACYT 20020220300118BA"]}]},{"name":"CONICET","award":["PICT 2020 SERIEA-03061"],"award-info":[{"award-number":["PICT 2020 SERIEA-03061"]}]},{"name":"CONICET","award":["PICT 2019 No. 3367"],"award-info":[{"award-number":["PICT 2019 No. 3367"]}]},{"name":"CONICET","award":["P123RT0138"],"award-info":[{"award-number":["P123RT0138"]}]},{"name":"Programa Iberoamericano de Ciencia y Tecnolog\u00eda para el Desarrollo (CYTED)","award":["UBACYT 20020220300118BA"],"award-info":[{"award-number":["UBACYT 20020220300118BA"]}]},{"name":"Programa Iberoamericano de Ciencia y Tecnolog\u00eda para el Desarrollo (CYTED)","award":["PICT 2020 SERIEA-03061"],"award-info":[{"award-number":["PICT 2020 SERIEA-03061"]}]},{"name":"Programa Iberoamericano de Ciencia y Tecnolog\u00eda para el Desarrollo (CYTED)","award":["PICT 2019 No. 3367"],"award-info":[{"award-number":["PICT 2019 No. 3367"]}]},{"name":"Programa Iberoamericano de Ciencia y Tecnolog\u00eda para el Desarrollo (CYTED)","award":["P123RT0138"],"award-info":[{"award-number":["P123RT0138"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Leishmaniasis is a neglected tropical disease caused by protozoan parasites of the genus Leishmania. An estimated 700,000 to 1 million new cases occur annually. Current therapies are limited by high toxicity, cost, prolonged treatment period, and rising resistance in endemic regions. The Asteraceae family has emerged as a promising source of bioactive compounds with proven leishmanicidal activity. In this study, the assessment of the antileishmanial activity of Helianthus tuberosus and Vernonanthura squamulosa extracts, the isolation of the sesquiterpene lactones heliangin and glaucolide A, respectively, and the evaluation of the activity of the compounds were conducted. Dichloromethane extracts of H. tuberosus and V. squamulosa were active on Leishmania amazonensis promastigotes, inhibiting the replication of the parasites in 97.2 \u00b1 3.1% and 89.1 \u00b1 1.1%, respectively, at 100 \u03bcg\/mL. Heliangin was active against promastigotes of L. amazonensis (IC50 = 9.3 \u03bcM) and intracellular amastigotes (IC50 = 0.8 \u03bcM), while glaucolide A exhibited moderate activity against promastigotes (IC50 = 46.7 \u03bcM) and did not show activity against intracellular amastigotes. Based on these results, heliangin was further evaluated in an animal model of cutaneous leishmaniasis using BALB\/c mice infected with L. amazonensis. Heliangin (8 mg\/Kg), when administered in combination with Glucantime, significantly reduced lesion progression and parasite load compared to the vehicle-treated group (p &lt; 0.001). These findings show that heliangin is a potential candidate for leishmaniasis treatment, especially in combination with therapeutic drugs.<\/jats:p>","DOI":"10.3390\/molecules30051039","type":"journal-article","created":{"date-parts":[[2025,2,25]],"date-time":"2025-02-25T03:40:12Z","timestamp":1740454812000},"page":"1039","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Bioactive Compounds with Leishmanicidal Potential from Helianthus tuberosus and Vernonanthura squamulosa"],"prefix":"10.3390","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-5901-9166","authenticated-orcid":false,"given":"Rachel","family":"N\u00e1poles Rodr\u00edguez","sequence":"first","affiliation":[{"name":"CONICET-Universidad de Buenos Aires, Instituto de Qu\u00edmica y Metabolismo del F\u00e1rmaco (IQUIMEFA), Autonomous City of Buenos Aires C1113AAD, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Laura","family":"Arreguez","sequence":"additional","affiliation":[{"name":"CONICET-Universidad Nacional de Salta, Instituto de Patolog\u00eda Experimental (IPE), Salta A4400, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-5603-8887","authenticated-orcid":false,"given":"Aldana M.","family":"Corlatti","sequence":"additional","affiliation":[{"name":"CONICET-Universidad de Buenos Aires, Instituto de Qu\u00edmica y Metabolismo del F\u00e1rmaco (IQUIMEFA), Autonomous City of Buenos Aires C1113AAD, Argentina"},{"name":"Universidad de Buenos Aires, Facultad de Farmacia y Bioqu\u00edmica, C\u00e1tedra de Farmacognosia, Autonomous City of Buenos Aires C1113AAD, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hern\u00e1n G.","family":"Bach","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Tecnolog\u00eda Agropecuaria, Buenos Aires B1686, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7409-5303","authenticated-orcid":false,"given":"C\u00e9sar A. N.","family":"Catal\u00e1n","sequence":"additional","affiliation":[{"name":"Instituto de Qu\u00edmica Org\u00e1nica, Facultad de Bioqu\u00edmica, Qu\u00edmica y Farmacia, Universidad Nacional de Tucum\u00e1n, San Miguel de Tucum\u00e1n, Tucum\u00e1n T4000INI, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura C.","family":"Laurella","sequence":"additional","affiliation":[{"name":"CONICET-Universidad de Buenos Aires, Instituto de Qu\u00edmica y Metabolismo del F\u00e1rmaco (IQUIMEFA), Autonomous City of Buenos Aires C1113AAD, Argentina"},{"name":"Universidad de Buenos Aires, Facultad de Farmacia y Bioqu\u00edmica, C\u00e1tedra de Farmacognosia, Autonomous City of Buenos Aires C1113AAD, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4406-2575","authenticated-orcid":false,"given":"Paola A.","family":"Barroso","sequence":"additional","affiliation":[{"name":"CONICET-Universidad Nacional de Salta, Instituto de Patolog\u00eda Experimental (IPE), Salta A4400, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9322-5748","authenticated-orcid":false,"given":"Valeria P.","family":"S\u00fclsen","sequence":"additional","affiliation":[{"name":"CONICET-Universidad de Buenos Aires, Instituto de Qu\u00edmica y Metabolismo del F\u00e1rmaco (IQUIMEFA), Autonomous City of Buenos Aires C1113AAD, Argentina"},{"name":"Universidad de Buenos Aires, Facultad de Farmacia y Bioqu\u00edmica, C\u00e1tedra de Farmacognosia, Autonomous City of Buenos Aires C1113AAD, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,24]]},"reference":[{"key":"ref_1","unstructured":"(2024, December 26). 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