{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T02:38:52Z","timestamp":1788921532306,"version":"build-2803163510"},"reference-count":35,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2025,3,16]],"date-time":"2025-03-16T00:00:00Z","timestamp":1742083200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Hainan Province Science and Technology Special Fund","award":["ZDYF2024XDNY170"],"award-info":[{"award-number":["ZDYF2024XDNY170"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>High-melting point 1,3-diacylglycerols not only provide health benefits, but are also suitable for manufacture of foods containing various specialty fats. It is difficult to prepare such high-melting point diacylglycerols, as the activities of specific enzymes will severely reduce at their melting points. In the present study, a combined technique was developed to prepare 1,3-dipalmitoylglycerol (1,3-DPG) and 1,3-distearoylglycerol (1,3-DSG) using selective esterification, molecular distillation, and solvent fractionation. Lipozyme TL IM was suitable for use as the optimal enzyme to maintain relatively high activity levels at esterification temperatures of 73\u201375 \u00b0C. 1,3-DAG\/(DAG + TAG) was selected as the most important index to monitor the esterification and to evaluate the synthesized fats. The obtained 1,3-DPG and 1,3-DSG showed high purities, at more than 83%, and possessed hard attributes at room temperature. Both 1,3-DPG and 1,3-DSG exhibited fat crystals with \u03b2\u2032 and \u03b2 crystals. Needle-like and rod-like crystals were observed at 5\u201325 \u00b0C for 1,3-DPG, and closely packed feather-like crystals were found at 5\u201320 \u00b0C for 1,3-DSG, indicating their multiple abilities in modifying the crystallization stabilization of the fat matrix during food processing.<\/jats:p>","DOI":"10.3390\/molecules30061328","type":"journal-article","created":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T04:29:28Z","timestamp":1742185768000},"page":"1328","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Enzymatic Esterification of Functional Lipids for Specialty Fats: 1,3-Dipalmitoylglycerol and 1,3-Distearoylglycerol"],"prefix":"10.3390","volume":"30","author":[{"given":"Yuhuang","family":"Yang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juanjuan","family":"Chi","sequence":"additional","affiliation":[{"name":"Wilmar (Shanghai) Biotechnology Research & Development Center Co., Ltd., Shanghai 200137, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengyuan","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdelaziz","family":"Elbarbary","sequence":"additional","affiliation":[{"name":"Dairy Science Department, Faculty of Agriculture, Benha University, Moshtohor 13736, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yafei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Wilmar (Shanghai) Biotechnology Research & Development Center Co., Ltd., Shanghai 200137, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6186-6882","authenticated-orcid":false,"given":"Jun","family":"Jin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China"},{"name":"Food Laboratory of Zhongyuan, Luohe 462300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1108","DOI":"10.1194\/jlr.M600524-JLR200","article-title":"Effects of triacylglycerol and diacylglycerol oils on blood clearance, tissue uptake, and hepatic apolipoprotein B secretion in mice","volume":"48","author":"Koichi","year":"2007","journal-title":"J. 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