{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T12:31:48Z","timestamp":1768480308530,"version":"3.49.0"},"reference-count":32,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2025,3,20]],"date-time":"2025-03-20T00:00:00Z","timestamp":1742428800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science KAKENHI","doi-asserted-by":"publisher","award":["24K09867"],"award-info":[{"award-number":["24K09867"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The screening of a small library of Japanese herbal tea extracts revealed significant anti-glycation activity in the leaves of Angelica keiskei. LC-MS analysis led to the identification of twenty compounds in this herb tea, including seven flavonoids, five phenylpropanoids, and eight coumarin derivatives, based on their chromatographic behavior and fragmentation patterns. Further LC-MS analysis of the methanol-eluted fraction after incubation with methylglyoxal (MGO) was performed on the reaction mixture, revealing quercetin 3-O-glucoside to be a key compound contributing to the anti-glycation activity of the leaves.<\/jats:p>","DOI":"10.3390\/molecules30061394","type":"journal-article","created":{"date-parts":[[2025,3,20]],"date-time":"2025-03-20T12:23:11Z","timestamp":1742473391000},"page":"1394","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Anti-Glycation Activities of Angelica keiskei Leaves"],"prefix":"10.3390","volume":"30","author":[{"given":"Yuno","family":"Takemoto","sequence":"first","affiliation":[{"name":"Faculty of Pharmaceutical Sciences, Toho University, Miyama 2-2-1, Funabashi 274-8510, Chiba, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8832-5720","authenticated-orcid":false,"given":"Takashi","family":"Kikuchi","sequence":"additional","affiliation":[{"name":"Faculty of Pharmaceutical Sciences, Toho University, Miyama 2-2-1, Funabashi 274-8510, Chiba, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenjun","family":"Qi","sequence":"additional","affiliation":[{"name":"Faculty of Pharmaceutical Sciences, Toho University, Miyama 2-2-1, Funabashi 274-8510, Chiba, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9780-136X","authenticated-orcid":false,"given":"Mi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Faculty of Pharmaceutical Sciences, Toho University, Miyama 2-2-1, Funabashi 274-8510, Chiba, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kouharu","family":"Otsuki","sequence":"additional","affiliation":[{"name":"Faculty of Pharmaceutical Sciences, Toho University, Miyama 2-2-1, Funabashi 274-8510, Chiba, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4143-8597","authenticated-orcid":false,"given":"Wei","family":"Li","sequence":"additional","affiliation":[{"name":"Faculty of Pharmaceutical Sciences, Toho University, Miyama 2-2-1, Funabashi 274-8510, Chiba, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1299","DOI":"10.2337\/dc15-2727","article-title":"Role of glycated proteins in the diagnosis and management of diabetes: Research gaps and future directions","volume":"39","author":"Welsh","year":"2016","journal-title":"Diabetes Care"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"969","DOI":"10.1002\/jms.842","article-title":"Mass spectrometric study of in vivo production of advanced glycation endproducts\/peptides","volume":"40","author":"Lapolla","year":"2005","journal-title":"J. Mass Spectrom."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1002\/mas.20090","article-title":"The role of mass spectrometry in the study of non-enzymatic protein glycation in diabetes: An update","volume":"25","author":"Lapolla","year":"2006","journal-title":"Mass Spectrom. Rev."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1021\/pr800858h","article-title":"A Perspective on the maillard reaction and the analysis of protein glycation by mass spectrometry: Probing the pathogenesis of chronic disease","volume":"8","author":"Zhang","year":"2009","journal-title":"J. Proteome Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1007\/s10719-021-09980-0","article-title":"Dicarbonyl stress, protein glycation and the unfolded protein response","volume":"38","author":"Rabbani","year":"2021","journal-title":"Glycoconj. J."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1152\/physrev.00001.2019","article-title":"Methylglyoxal, a highly reactive dicarbonyl compound, in diabetes, its vascular complications, and other age-related diseases","volume":"100","author":"Schalkwijk","year":"2020","journal-title":"Physiol. Rev."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Stratmann, B. (2022). Stress in diabetic vascular disease. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23116186"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1614","DOI":"10.1016\/j.drudis.2020.07.003","article-title":"Advanced glycation end products in diabetes, cancer and phytochemical therapy","volume":"25","author":"Dariya","year":"2020","journal-title":"Drug Discov. Today"},{"key":"ref_9","unstructured":"Mitsuhashi, H. (1988). Illustrated Medicinal Plants of the World in Clour, Hokuryukan Co., Ltd."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1007\/s12272-017-0892-3","article-title":"Angelica keiskei, an emerging medicinal herb with various bioactive constituents and biological activities","volume":"40","author":"Kil","year":"2017","journal-title":"Arch. Pharmacal Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1007\/s12272-011-0311-0","article-title":"Chalcones isolated from Angelica keiskei and their inhibition of IL-6 production in TNF-\u03b1-stimulated MG-63 cell","volume":"34","author":"Shin","year":"2011","journal-title":"Arch. Pharmacal Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.foodchem.2013.12.026","article-title":"Quantitative analysis of phenolic metabolites from different parts of Angelica keiskei by HPLC-ESI MS\/MS and their xanthine oxidase inhibition","volume":"153","author":"Kim","year":"2014","journal-title":"Food Chem."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"4406","DOI":"10.1007\/s13197-018-3348-y","article-title":"Phytonutrient and anti-diabetic functional properties of flavonoid-rich ethanol extract from Angelica keiskei leaves","volume":"55","author":"Zhang","year":"2018","journal-title":"J. Food Sci. Technol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"6013","DOI":"10.1021\/jf070720q","article-title":"Antidiabetic Activities of Chalcones Isolated from a Japanese Herb, Angelica keiskei","volume":"55","author":"Enoki","year":"2007","journal-title":"J. Agric. Food Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2028","DOI":"10.1016\/j.bmcl.2015.04.003","article-title":"PTP1B inhibitors from stems of Angelica keiskei (Ashitaba)","volume":"25","author":"Li","year":"2015","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"ref_16","first-page":"1655","article-title":"Effects of Angelica keiskei chalcone on insulin resistance of skeletal muscle cells of type 2 diabetic rats","volume":"13","author":"Zhao","year":"2013","journal-title":"Xiandai Shengwuyixue Jinzhan"},{"key":"ref_17","unstructured":"Akaki, J., and Kojima, H. (2008). Glycation Inhibitor. (WO 2008038705 A1)."},{"key":"ref_18","first-page":"58","article-title":"Isolation and characterization of antioxidative compounds from the aerial parts of Angelica keiskei","volume":"14","author":"Kim","year":"2005","journal-title":"Food Sci. Biotechnol."},{"key":"ref_19","first-page":"221","article-title":"Further isolation of coumarin from Angelica pubescens Maxim f. Shan et Yuan","volume":"6","author":"Liu","year":"1997","journal-title":"J. Chin. Pharm. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"105758","DOI":"10.1016\/j.fitote.2023.105758","article-title":"Systematic characterization of the chemical constituents in vitro and in vivo of Qianghuo by UPLC-Q-TOF-MS\/MS","volume":"172","author":"Wu","year":"2024","journal-title":"Fitoterapia"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Tian, Z., Sun, L., Chi, B., Du, Z., Zhang, X., Liu, Y., and Zhou, H. (2022). Affinity ultrafiltration and UPLC-HR-Orbitrap-MS based screening of neuraminidase inhibitors from Angelica pubescens. J. Chromatogr. B Anal. Technol. Biomed. Life Sci., 1208.","DOI":"10.1016\/j.jchromb.2022.123398"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"992","DOI":"10.1016\/j.foodchem.2011.09.099","article-title":"Compounds from Angelica keiskei with NQO1 induction, DPPH\u00b7 scavenging and \u03b1-glucosidase inhibitory activities","volume":"131","author":"Luo","year":"2012","journal-title":"Food Chem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/S0304-3835(03)00466-X","article-title":"Chalcones, coumarins, and flavanones from the exudate of Angelica keiskei and their chemopreventive effects","volume":"201","author":"Akihisa","year":"2003","journal-title":"Cancer Lett."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1021\/np058080d","article-title":"Chalcones and other compounds from the exudates of Angelica keiskei and their cancer chemopreventive effects","volume":"69","author":"Akihisa","year":"2006","journal-title":"J. Nat. Prod."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.foodchem.2008.12.015","article-title":"Characterisation, extraction efficiency, stability and antioxidant activity of phytonutrients in Angelica keiskei","volume":"115","author":"Li","year":"2009","journal-title":"Food Chem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"100033","DOI":"10.1016\/j.fochx.2019.100033","article-title":"Comprehensive characterization of hydroxycinnamoyl derivatives in green and roasted coffee beans: A new group of methyl hydroxycinnamoyl quinate","volume":"2","author":"Asamenew","year":"2019","journal-title":"Food Chem. X"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Ding, M., Bai, Y., Li, J., Yang, X., Wang, H., Gao, X., and Chang, Y.-X. (2019). A diol-based-matrix solid-phase dispersion method for the simultaneous extraction and determination of 13 compounds from Angelicae Pubescentis Radix by ultra high-performance liquid chromatography. Front. Pharmacol., 10.","DOI":"10.3389\/fphar.2019.00227"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2300473","DOI":"10.1002\/jssc.202300473","article-title":"Chemical composition analysis of Angelica sinensis (Oliv.) Diels and its four processed products by ultra-high-performance liquid chromatography coupled with quadrupole-orbitrap mass spectrometry combining with nontargeted metabolomics","volume":"46","author":"Wang","year":"2023","journal-title":"J. Sep. Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"3202","DOI":"10.1021\/jf500204s","article-title":"Essential Structural Requirements and Additive Effects for Flavonoids to Scavenge Methylglyoxal","volume":"62","author":"Shao","year":"2014","journal-title":"J. Agric. Food Chem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.jff.2017.05.043","article-title":"Flavonoid glycosides from Japanese Camellia oil cakes and their inhibitory activity against advanced glycation end-products formation","volume":"35","author":"Sato","year":"2017","journal-title":"J. Funct. Foods"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"132008","DOI":"10.1016\/j.foodchem.2021.132008","article-title":"Identification and cytotoxic evaluation of the novel rutin-methylglyoxal adducts with dione structures in vivo and in foods","volume":"377","author":"Chen","year":"2022","journal-title":"Food Chem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"20374","DOI":"10.1021\/acs.jafc.4c01637","article-title":"Scavenging of Methylglyoxal by the Total Flavonoids of Apocyni Veneti Folium in Mice","volume":"72","author":"Zhou","year":"2024","journal-title":"J. Agric. Food Chem."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/30\/6\/1394\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T16:57:25Z","timestamp":1760029045000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/30\/6\/1394"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,20]]},"references-count":32,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2025,3]]}},"alternative-id":["molecules30061394"],"URL":"https:\/\/doi.org\/10.3390\/molecules30061394","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,20]]}}}