{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:07:49Z","timestamp":1760058469096,"version":"build-2065373602"},"reference-count":27,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2025,4,2]],"date-time":"2025-04-02T00:00:00Z","timestamp":1743552000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Qiqihar Science and Technology Plan Joint Guidance Project","award":["LSFGG-2023038","LBH-Z24307","2022QNTJ019","QYZDXK-001"],"award-info":[{"award-number":["LSFGG-2023038","LBH-Z24307","2022QNTJ019","QYZDXK-001"]}]},{"name":"Heilongjiang Postdoctoral Fund","award":["LSFGG-2023038","LBH-Z24307","2022QNTJ019","QYZDXK-001"],"award-info":[{"award-number":["LSFGG-2023038","LBH-Z24307","2022QNTJ019","QYZDXK-001"]}]},{"name":"Young Elite Scientist Sponsorship Program by Heilongjiang Province","award":["LSFGG-2023038","LBH-Z24307","2022QNTJ019","QYZDXK-001"],"award-info":[{"award-number":["LSFGG-2023038","LBH-Z24307","2022QNTJ019","QYZDXK-001"]}]},{"name":"Construction Project of Dominant Characteristic Disciplines of Qiqihar Medical University","award":["LSFGG-2023038","LBH-Z24307","2022QNTJ019","QYZDXK-001"],"award-info":[{"award-number":["LSFGG-2023038","LBH-Z24307","2022QNTJ019","QYZDXK-001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Local anesthetics have been widely used in clinical analgesia due to their ability to provide effective regional pain management. Accurate measurement of local anesthetics in body fluids is crucial for ensuring patient medication safety and optimizing therapeutic efficacy. Herein, we present a convenient, economical, sensitive, and efficient TLC-SERS method for multiplex determination of six kinds of anesthetics (pro) in human plasma, including procaine hydrochloride (Pro), tetracaine hydrochloride (Tet), dibucaine (Dib), mepivacaine hydrochloride (Mep), lidocaine hydrochloride (Lid), and ropivacaine hydrochloride (Rop). The TLC method was adopted to separate six local anesthetics effectively. In order to improve the sensitivity, TLC spots were concentrated into smaller ones using methanol through solvent-driven enrichment, then Ag NPs staining was applied to enriched spots for a strong and unique SERS response of each anesthetic. As a result, linear calibration curves of SERS intensity ratio versus negative logarithm of spotting amounts sampled on TLC plates were obtained, along with the lowest detectable amounts in this study were 1 ng (Pro), 10 pg (Tet), 10 ng (Dib), 50 ng (Mep), 50 ng (Lid), and 0.1 \u03bcg (Rop), which were up to 2 \u00d7 104 times more sensitive than our previous TLC-Raman method. Moreover, the method was successfully applied to human plasma samples, demonstrating the feasibility and potential for multiplex analysis of local anesthetics in clinical practice, criminal forensics, and aquaculture.<\/jats:p>","DOI":"10.3390\/molecules30071585","type":"journal-article","created":{"date-parts":[[2025,4,2]],"date-time":"2025-04-02T07:47:44Z","timestamp":1743580064000},"page":"1585","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Solvent-Driven Enrichment and Multiplex Analysis of Local Anesthetics by Thin-Layer Chromatography Coupled with Surface-Enhanced Raman Spectroscopy"],"prefix":"10.3390","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6289-6143","authenticated-orcid":false,"given":"Huimin","family":"Sui","sequence":"first","affiliation":[{"name":"School of Pharmacy, Qiqihar Medical University, Qiqihar 161006, China"},{"name":"Postdoctoral Research Station, Qiqihar Institute of Medical and Pharmaceutical Sciences, Qiqihar 161006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miao","family":"Li","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Qiqihar Medical University, Qiqihar 161006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yangyang","family":"Gao","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Qiqihar Medical University, Qiqihar 161006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Luo","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Qiqihar Medical University, Qiqihar 161006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fangyuan","family":"Ban","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Qiqihar Medical University, Qiqihar 161006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Qiqihar Medical University, Qiqihar 161006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuang","family":"Fu","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Qiqihar Medical University, Qiqihar 161006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao-Yang","family":"Zhao","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Qiqihar Medical University, Qiqihar 161006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hailin","family":"Wen","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Qiqihar Medical University, Qiqihar 161006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cuiyan","family":"Han","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Qiqihar Medical University, Qiqihar 161006, China"},{"name":"Postdoctoral Research Station, Qiqihar Institute of Medical and Pharmaceutical Sciences, Qiqihar 161006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/S0889-8537(05)70161-9","article-title":"The pharmacology of local anesthetics","volume":"18","author":"Tetzlaff","year":"2000","journal-title":"Anesthesiol. 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