{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T02:53:08Z","timestamp":1780368788791,"version":"3.54.1"},"reference-count":66,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,4,18]],"date-time":"2025-04-18T00:00:00Z","timestamp":1744934400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Non-enzymatic antioxidants, such as polyphenols, can counteract free radicals and other potentially toxic oxidants produced by marine plants exposed to stress. In this study, we assessed different methods for measuring antioxidant capacities and condensed tannins in the seagrass Posidonia oceanica (L.) Delile. Two polyphenol extraction methods, direct and sequential, were compared to determine their efficiencies. Condensed tannins were assayed directly on leaf flour using a modified HCl-butanol-acetone-iron reagent method. Total antioxidant capacities were assayed with the ABTS, CUPRAC, and ORAC methods, both on extracts and on powdered samples (QUENCHER). The direct assays showed higher sensitivity compared to their in-solution counterparts. Our results indicate that in-depth measurement of antioxidant compounds and capacities can be achieved by direct assays on P. oceanica powder samples, and these data can be used to assess changes in the plant biochemistry due to the exposure to varying biotic and abiotic conditions.<\/jats:p>","DOI":"10.3390\/molecules30081828","type":"journal-article","created":{"date-parts":[[2025,4,20]],"date-time":"2025-04-20T20:15:06Z","timestamp":1745180106000},"page":"1828","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["A Comparison of Methods for Assessing the Antioxidant Expression in Posidonia oceanica (L.) Delile"],"prefix":"10.3390","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5519-993X","authenticated-orcid":false,"given":"Debora","family":"Fontanini","sequence":"first","affiliation":[{"name":"Department of Biology, University of Pisa, Via L. Ghini 13, 56126 Pisa, Italy"},{"name":"MARinePHARMA Center, University of Pisa, Via Bonanno Pisano 6, 56126 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7335-6791","authenticated-orcid":false,"given":"Fabio","family":"Bulleri","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Pisa, Via L. Ghini 13, 56126 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Ravaglioli","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Pisa, Via L. Ghini 13, 56126 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonella","family":"Capocchi","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Pisa, Via L. Ghini 13, 56126 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,18]]},"reference":[{"key":"ref_1","unstructured":"Larkum, A.W.D., Orth, R.J., and Duarte, C.M. (2007). Biology of Posidonia. Seagrasses: Biology, Ecology and Conservation, Springer."},{"key":"ref_2","unstructured":"Habitats Directive (1992). Council Directive 92\/43\/EEC of 21 May 1992 on the conservation of natural habitats and of wild fauna and flora. Off. J. Eur. Union, 206, 7\u201350."},{"key":"ref_3","first-page":"105557","article-title":"Posidonia oceanica L. (Delile) meadows regression: Long-term affection may be induced by multiple impacts","volume":"174","year":"2002","journal-title":"Mar. Environ. Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.marpolbul.2016.06.022","article-title":"From mechanical to chemical impact of anchoring in seagrasses: The premises of anthropogenic patch generation in Posidonia oceanica meadows","volume":"109","author":"Abadie","year":"2006","journal-title":"Mar. Pollut. Bull"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.aquabot.2014.01.006","article-title":"Getting turfed: The population and habitat impacts of Lophocladia lallemandii invasions on endemic Posidonia oceanica meadows","volume":"116","author":"Arthur","year":"2014","journal-title":"Aquat. Bot."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.biocon.2014.05.024","article-title":"Mediterranean seagrass (Posidonia oceanica) loss between 1842 and 2009","volume":"176","author":"Duarte","year":"2014","journal-title":"Biol. Conserv."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"3356","DOI":"10.1038\/s41467-019-11340-4","article-title":"Recent trend reversal for declining European seagrass meadows","volume":"10","author":"Alcoverro","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Pazzaglia, J., Santill\u00e1n-Sarmiento, A., Helber, S.B., Ruocco, M., Terlizzi, A., Mar\u00edn-Guirao, L., and Procaccini, G. (2020). Does warming enhance the effects of eutrophication in the seagrass Posidonia oceanica?. Front. Mar. Sci., 7.","DOI":"10.3389\/fmars.2020.564805"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"89","DOI":"10.3354\/meps236089","article-title":"Depth-acclimation of photosynthesis, morphology and demography of Posidonia oceanica and Cymodocea nodosa in the Spanish Mediterranean Sea","volume":"236","author":"Olesen","year":"2002","journal-title":"Mar. Ecol. Prog. Ser."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/j.marenvres.2014.07.010","article-title":"Response of the seagrass Posidonia oceanica to different light environments: Insights from a combined molecular and photo-physiological study","volume":"101","author":"Dattolo","year":"2014","journal-title":"Mar. Environ. Res."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Procaccini, G., Ruocco, M., Mar\u00edn-Guirao, L., Dattolo, E., Brunet, C., D\u2019Esposito, D., Lauritano, C., Mazzuca, S., Serra, I.A., and Bernardo, L. (2017). Depth-specific fluctuations of gene expression and protein abundance modulate the photophysiology in the seagrass Posidonia oceanica. Sci. Rep., 7.","DOI":"10.1038\/srep42890"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1111\/1365-2745.13479","article-title":"A king and vassals\u2019 tale: Molecular signatures of clonal integration in Posidonia oceanica under chronic light shortage","volume":"109","author":"Ruocco","year":"2021","journal-title":"J. Ecol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"856","DOI":"10.1111\/tpj.13299","article-title":"Reactive oxygen species, abiotic stress and stress combination","volume":"90","author":"Choudhury","year":"2017","journal-title":"Plant J."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1146\/annurev-arplant-071720-015522","article-title":"Dissipation of light energy absorbed in excess: The molecular mechanisms","volume":"72","author":"Bassi","year":"2021","journal-title":"Ann. Rev. Plant Biol."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Lingvay, M., Akhtar, P., Seb\u0151k-Nagy, K., P\u00e1li, T., and Lambrev, P.H. (2020). Photobleaching of chlorophyll in light-harvesting complex II increases in lipid environment. Front. Plant Sci., 11.","DOI":"10.3389\/fpls.2020.00849"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/S1360-1385(97)01018-2","article-title":"Antioxidant properties of phenolic compounds","volume":"2","author":"Miller","year":"1997","journal-title":"Trends Plant Sci."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Zeb, A. (2020). Concept, mechanism, and applications of phenolic antioxidants in foods. J. Food Biochem., 44.","DOI":"10.1111\/jfbc.13394"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1016\/S0031-9422(97)01118-7","article-title":"Distribution of phenolic compounds in the seagrass Posidonia oceanica","volume":"48","author":"Agostini","year":"1998","journal-title":"Phytochemistry"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1071\/FP18303","article-title":"Physiological response of Posidonia oceanica to heavy metal pollution along the Tyrrhenian coast","volume":"46","author":"Bertini","year":"2019","journal-title":"Funct. Plant Biol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"139601","DOI":"10.1016\/j.scitotenv.2020.139601","article-title":"Hypersaline water from desalinization plants causes oxidative damage in Posidonia oceanica meadows","volume":"736","author":"Tejada","year":"2020","journal-title":"Sci. Total Environ."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Costa, M.M., Barrote, I., Silva, J., Oliv\u00e9, I., Alexandre, A., Albano, S., and Santos, R. (2015). Epiphytes modulate Posidonia oceanica photosynthetic production, energetic balance, antioxidant mechanisms, and oxidative damage. Front. Mar. Sci., 2.","DOI":"10.3389\/fmars.2015.00111"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"3211","DOI":"10.1016\/j.phytochem.2004.09.003","article-title":"Variations in the concentration of phenolic compounds in the seagrass Posidonia oceanica under conditions of competition","volume":"65","author":"Dumay","year":"2004","journal-title":"Phytochemistry"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"114507","DOI":"10.1016\/j.marpolbul.2022.114507","article-title":"Sunscreen exposure interferes with physiological processes while inducing oxidative stress in seagrass Posidonia oceanica (L.) Delile","volume":"187","author":"Agawin","year":"2023","journal-title":"Mar. Pollut. Bull."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Mannino, A.M., and Micheli, C. (2020). Ecological function of phenolic compounds from mediterranean fucoid algae and seagrasses: An overview on the genus Cystoseira sensu lato and Posidonia oceanica (L.) Delile. J. Mar. Sci. Eng., 8.","DOI":"10.3390\/jmse8010019"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.marenvres.2018.02.019","article-title":"Macro-grazer herbivory regulates seagrass response to pulse and press nutrient loading","volume":"136","author":"Ravaglioli","year":"2018","journal-title":"Mar. Environ. Res."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Garc\u00eda-M\u00e1rquez, M.G., Fern\u00e1ndez-Ju\u00e1rez, V., Rodr\u00edguez-Casta\u00f1eda, J.C., and Agawin, N.S. (2022). Response of Posidonia oceanica (L.) Delile and its associated N2 fixers to different combinations of temperature and light levels. Front. Mar. Sci., 8.","DOI":"10.3389\/fmars.2021.757572"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"117629","DOI":"10.1016\/j.envres.2023.117629","article-title":"Ocean acidification impairs seagrass performance under thermal stress in shallow and deep water","volume":"241","author":"Ravaglioli","year":"2024","journal-title":"Environ. Res."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Kesraoui, O., Marzouki, M.N., Maugard, T., and Limam, F. (2011). In vitro evaluation of antioxidant activities of free and bound phenolic compounds from Posidonia oceanica (L.) Delile leaves. Afr. J. Biotechnol., 10.","DOI":"10.5897\/AJB10.847"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1080\/19336918.2015.1008330","article-title":"Hydrophilic extract from Posidonia oceanica inhibits activity and expression of gelatinases and prevents HT1080 human fibrosarcoma cell line invasion","volume":"9","author":"Barletta","year":"2015","journal-title":"Cell Adh. Migr."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Leri, M., Ramazzotti, M., Vasarri, M., Peri, S., Barletta, E., Pretti, C., and Degl\u2019Innocenti, D. (2018). Bioactive Compounds from Posidonia oceanica (L.) Delile Impair Malignant Cell Migration through Autophagy Modulation. Mar. Drugs, 16.","DOI":"10.3390\/md16040137"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Cornara, L., Pastorino, G., Borghesi, B., Salis, A., Clericuzio, M., Marchetti, C., Damonte, G., and Burlando, B. (2018). Posidonia oceanica (L.) Delile Ethanolic Extract Modulates Cell Activities with Skin Health Applications. Mar. Drugs, 16.","DOI":"10.3390\/md16010021"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"112252","DOI":"10.1016\/j.jep.2019.112252","article-title":"Anti-inflammatory properties of the marine plant Posidonia oceanica (L.) Delile","volume":"247","author":"Vasarri","year":"2020","journal-title":"J. Ethnopharmacol."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Messina, C.M., Arena, R., Manuguerra, S., Pericot, Y., Curcuraci, E., Kerninon, F., Renda, G., Hellio, C., and Santulli, A. (2021). Antioxidant Bioactivity of Extracts from Beach Cast Leaves of Posidonia oceanica (L.) Delile. Mar. Drugs, 19.","DOI":"10.3390\/md19100560"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Sharma, A., Shahzad, B., Rehman, A., Bhardwaj, R., Landi, M., and Zheng, B. (2019). Response of Phenylpropanoid Pathway and the Role of Polyphenols in Plants under Abiotic Stress. Molecules, 24.","DOI":"10.3390\/molecules24132452"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"e09094","DOI":"10.1016\/j.heliyon.2022.e09094","article-title":"Reinforcing the bulwark: Unravelling the efficient applications of plant phenolics and tannins against environmental stresses","volume":"8","author":"Dehghanian","year":"2022","journal-title":"Heliyon"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"6910","DOI":"10.1021\/jf020665f","article-title":"Antioxidant and antiproliferative activity of common vegetables","volume":"50","author":"Chu","year":"2002","journal-title":"J. Agric. Food Chem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1021\/jf00110a028","article-title":"Free, esterified, and insoluble bound phenolic-acids. 1. Extraction and purification procedure","volume":"30","author":"Krygier","year":"1982","journal-title":"J. Agric. Food Chem."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Astudillo-Pascual, M., Dom\u00ednguez, I., Aguilera, P.A., and Garrido Frenich, A. (2021). New Phenolic Compounds in Posidonia oceanica Seagrass: A Comprehensive Array Using High Resolution Mass Spectrometry. Plants, 10.","DOI":"10.3390\/plants10050864"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1016\/j.trac.2016.03.010","article-title":"Chemical perspective and criticism on selected analytical methods used to estimate the total content of phenolic compounds in food matrices","volume":"80","author":"Granato","year":"2016","journal-title":"Trends Anal. Chem."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"17543","DOI":"10.1021\/acs.jafc.3c04022","article-title":"The Chemistry Behind the Folin-Ciocalteu Method for the Estimation of (Poly)phenol Content in Food: Total Phenolic Intake in a Mediterranean Dietary Pattern","volume":"71","year":"2023","journal-title":"J. Agric. Food. Chem."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1515\/bot-2014-0098","article-title":"Phenolic fingerprint of the seagrass Posidonia oceanica from four locations in the Mediterranean Sea: First evidence for the large predominance of chicoric acid","volume":"58","author":"Rezzonico","year":"2015","journal-title":"Bot. Mar."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1083","DOI":"10.1007\/s10886-007-9290-5","article-title":"The influence of tissue handling on the flavonoid content of the aquatic plant Posidonia oceanica","volume":"33","author":"Cannac","year":"2007","journal-title":"J. Chem. Ecol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.scitotenv.2006.07.016","article-title":"Effects of fish farming on flavonoids in Posidonia oceanica","volume":"370","author":"Cannac","year":"2006","journal-title":"Sci. Total Environ."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.foodchem.2014.08.038","article-title":"Effect of esterification condensation on the Folin\u2013Ciocalteu method for the quantitative measurement of total phenols","volume":"170","author":"Chen","year":"2015","journal-title":"Food Chem."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"111932","DOI":"10.1016\/j.lwt.2021.111932","article-title":"Determination of total flavonoid content by aluminum chloride assay: A critical evaluation","volume":"150","author":"Shraim","year":"2021","journal-title":"LWT"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1919","DOI":"10.1023\/A:1020737609151","article-title":"Marine tannins: The importance of a mechanistic framework for predicting ecological roles","volume":"28","author":"Arnold","year":"2002","journal-title":"J. Chem. Ecol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"879","DOI":"10.12681\/mms.30709","article-title":"Patterns of Posidonia oceanica (L.) Delile tannin cells and leaf characteristics according to environmental conditions","volume":"23","author":"Boumaza","year":"2022","journal-title":"Mediterr. Mar. Sci."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"2906","DOI":"10.1021\/acs.jafc.9b01307","article-title":"Direct versus Sequential Analysis of Procyanidin- and Prodelphinidin-Based Condensed Tannins by the HCl\u2212Butanol\u2212Acetone\u2212Iron Assay","volume":"68","author":"Grabber","year":"2020","journal-title":"J. Agric. Food Chem."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1186\/s13007-017-0213-3","article-title":"An improved butanol-HCl assay for quantification of water-soluble, acetone:methanol-soluble, and insoluble proanthocyanidins (condensed tannins)","volume":"13","author":"Shay","year":"2017","journal-title":"Plant Methods"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/S0031-9422(00)94533-3","article-title":"The Conversion of Procyanidins and Prodelphinidins to Cyanidin and Delphinidin","volume":"25","author":"Porter","year":"1986","journal-title":"Phytochem."},{"key":"ref_51","first-page":"8","article-title":"Powdered hide model for vegetable tanning","volume":"109","author":"Brown","year":"2014","journal-title":"J. Am. Leather Chem. Assoc."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.phytochem.2011.10.006","article-title":"Analysis of commercial proanthocyanidins. Part 1: The chemical composition of quebracho (Schinopsis lorentzii and Schinopsis balansae) heartwood extract","volume":"73","author":"Venter","year":"2012","journal-title":"Phytochemistry"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1007\/s10886-010-9784-4","article-title":"Preparation of an acid butanol standard from fresh apples","volume":"36","author":"Li","year":"2010","journal-title":"J. Chem. Ecol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"4021","DOI":"10.1021\/jf063061a","article-title":"Structural Constituents of the Seagrass Posidonia australis","volume":"55","author":"Torbatinejad","year":"2007","journal-title":"J. Agric. Food Chem."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"125474","DOI":"10.1016\/j.talanta.2023.125474","article-title":"The power of the QUENCHER method in measuring total antioxidant capacity of foods: Importance of interactions between different forms of antioxidants","volume":"269","year":"2024","journal-title":"Talanta"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1016\/j.tifs.2009.03.010","article-title":"Direct measurement of the total antioxidant capacity of foods: The \u2018QUENCHER\u2019 approach","volume":"20","author":"Serpen","year":"2009","journal-title":"Trends Food Sci. Technol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1021\/acs.jafc.5b04739","article-title":"Antioxidant Activity\/Capacity Measurement. 1. Classification, Physicochemical Principles, Mechanisms, and Electron Transfer (ET)-Based Assays","volume":"64","author":"Apak","year":"2016","journal-title":"J. Agric. Food. Chem."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"144","DOI":"10.5344\/ajev.1965.16.3.144","article-title":"Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents","volume":"16","author":"Singleton","year":"1965","journal-title":"Am. J. Enol. Vitic."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"3010","DOI":"10.1021\/jf0115589","article-title":"Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity","volume":"50","author":"Dewanto","year":"2002","journal-title":"J. Agric. Food Chem."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1016\/S0891-5849(98)00315-3","article-title":"Antioxidant activity applying an improved ABTS radical cation decolorization assay","volume":"26","author":"Re","year":"1999","journal-title":"Free Radical Biol. Med."},{"key":"ref_61","first-page":"1","article-title":"Metodologia Cientifica: Determinacao da Atividade Antioxidanta Total em Frutas pela Captura do Radical Livre ABTS+","volume":"128","author":"Rufino","year":"2007","journal-title":"Comun. Tec."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.talanta.2013.02.061","article-title":"Direct measurement of total antioxidant capacity of cereals: QUENCHER-CUPRAC method","volume":"108","author":"Tufan","year":"2013","journal-title":"Talanta"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"7970","DOI":"10.1021\/jf048741x","article-title":"Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method","volume":"52","author":"Apak","year":"2004","journal-title":"J. Agric. Food Chem."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"4437","DOI":"10.1021\/jf0201529","article-title":"High-Throughput Assay of Oxygen Radical Absorbance Capacity (ORAC) Using a Multichannel Liquid Handling System Coupled with a Microplate Fluorescence Reader in 96-Well Format","volume":"50","author":"Huang","year":"2002","journal-title":"J. Agric. Food Chem."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1007\/s00217-010-1308-5","article-title":"Are the major antioxidants derived from soy protein and fructo-oligosaccharides model systems colored aqueous soluble or insoluble compounds?","volume":"231","author":"Fogliano","year":"2010","journal-title":"Eur. Food Res. Technol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1372","DOI":"10.5740\/jaoacint.13-175","article-title":"Determination of Total Antioxidant Capacity by Oxygen Radical Absorbance Capacity (ORAC) Using Fluorescein as the Fluorescence Probe: First Action 2012.23","volume":"6","author":"Ou","year":"2013","journal-title":"J. AOAC Int."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/30\/8\/1828\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:17:35Z","timestamp":1760030255000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/30\/8\/1828"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,18]]},"references-count":66,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2025,4]]}},"alternative-id":["molecules30081828"],"URL":"https:\/\/doi.org\/10.3390\/molecules30081828","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,18]]}}}