{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T02:04:45Z","timestamp":1767924285829,"version":"3.49.0"},"reference-count":42,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T00:00:00Z","timestamp":1745452800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Here, we examined the antioxidant and anti-inflammatory activities of the ethyl acetate (EtOAc) fraction of Astilboides tabularis (Hemsl.) Engl. root extracts, initially prepared from a 70% ethanol extraction. This EtOAc fraction exhibited significant scavenging activity against DPPH radicals (IC50: 11.38 \u00b1 0.48 \u00b5g\/mL) and ABTS radicals (IC50: 7.46 \u00b1 0.58 \u00b5g\/mL), and had a high total phenolic content (i.e., 407.02 \u00b1 13.56 mg GAE\/g). In addition, the EtOAc fraction demonstrated concentration-dependent protective effects in a RAW264.7 macrophage cell model subjected to oxidative stress. In lipopolysaccharide (LPS)-stimulated RAW264.7 cells, nitric oxide (NO) production and the expression of inflammatory mediators (iNOS, COX-2, TNF-\u03b1, IL-1\u03b2, IFN-\u03b2) were inhibited in a concentration-dependent manner. Western blot and real-time PCR (RT-PCR) analyses revealed that the EtOAc fraction also suppressed inflammatory mediator expression via inhibiting the activation of the NF-\u03baB and MAPK signaling pathways. Finally, LC-QTOF-MS and LC-MS\/MS analyses identified gallic acid and bergenin as compounds contributing to observed antioxidant and anti-inflammatory effects. In conclusion, the EtOAc fraction of A. tabularis root extracts exhibited strong anti-oxidant and anti-inflammatory properties, suggesting potential usage for treating various inflammatory diseases.<\/jats:p>","DOI":"10.3390\/molecules30091892","type":"journal-article","created":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T06:18:02Z","timestamp":1745475482000},"page":"1892","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Antioxidant and Anti-Inflammatory Activities of Astilboides tabularis (Hemsl.) Engl. Root Extract"],"prefix":"10.3390","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5306-7418","authenticated-orcid":false,"given":"Nam Ho","family":"Yoo","sequence":"first","affiliation":[{"name":"Department of Bio-Resource Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9471-2567","authenticated-orcid":false,"given":"Young Sun","family":"Baek","sequence":"additional","affiliation":[{"name":"Department of Bio-Resource Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9390-6802","authenticated-orcid":false,"given":"Hee Kyu","family":"Kim","sequence":"additional","affiliation":[{"name":"Gangwondo Forest Science Institute, Chuncheon 24207, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3153-7938","authenticated-orcid":false,"given":"Chan Ok","family":"Lee","sequence":"additional","affiliation":[{"name":"Gangwondo Forest Science Institute, Chuncheon 24207, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4378-4071","authenticated-orcid":false,"given":"Myong Jo","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Bio-Resource Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1345","DOI":"10.3746\/jkfn.2019.48.12.1345","article-title":"Analysis of the Functional Components and Various Biological Activities of Korean Medicinal Herbs","volume":"48","author":"Park","year":"2019","journal-title":"J. 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