{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T19:50:57Z","timestamp":1790884257443,"version":"4.1.0"},"reference-count":37,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2025,5,6]],"date-time":"2025-05-06T00:00:00Z","timestamp":1746489600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["82060787"],"award-info":[{"award-number":["82060787"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Hepatitis B is a serious infectious disease that threatens the health of all mankind. In this study, we isolated and extracted hydroxytyrosol from Lindernia ruellioides with anti-hepatitis B virus (HBV) activity. The structure of hydroxytyrosol was identified by the nuclear magnetic resonance technique. HepG2.2.15 cell models were used to detect the anti-HBV activity and liver protection of hydroxytyrosol in vitro. Hydroxytyrosol can inhibit hepatitis B surface antigen (HBsAg) and hepatitis B e-antigen (HBeAg). The IC50 values of HBsAg and HBeAg were 4.02 mg\/L and 5.19 mg\/L, respectively. At the highest concentration of hydroxytyrosol, the inhibition rates of supernatant and intracellular HBV DNA were 75.99% and 66.33%, respectively. Hydroxytyrosol was less toxic to normal human hepatocytes. Molecular docking showed that hydroxytyrosol was bound to three amino acid residues of HBV polymerase with a binding energy of \u22127.0 kcal\/mol. This study provided data for the development and utilization of Lindernia ruellioides and the research and development of anti-hepatitis B virus drugs.<\/jats:p>","DOI":"10.3390\/molecules30092063","type":"journal-article","created":{"date-parts":[[2025,5,6]],"date-time":"2025-05-06T09:08:56Z","timestamp":1746522536000},"page":"2063","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["In Vitro Anti-Hepatitis B Virus Activity of Hydroxytyrosol from Lindernia ruellioides"],"prefix":"10.3390","volume":"30","author":[{"given":"Tong-Shi-Yao","family":"Zhao","sequence":"first","affiliation":[{"name":"Key Laboratory of Molecular Medical Engineering, Education Department of Guangxi Zhuang Autonomous Region, Guilin Medical University, Guilin 541100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kang-Zhi","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Medical Engineering, Education Department of Guangxi Zhuang Autonomous Region, Guilin Medical University, Guilin 541100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"He-Ling","family":"Su","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Medical Engineering, Education Department of Guangxi Zhuang Autonomous Region, Guilin Medical University, Guilin 541100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Liang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Medical Engineering, Education Department of Guangxi Zhuang Autonomous Region, Guilin Medical University, Guilin 541100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng-Qin","family":"Liang","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Guilin Medical University, Guilin 541100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3991-9706","authenticated-orcid":false,"given":"Jin-Tao","family":"Gao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Medical Engineering, Education Department of Guangxi Zhuang Autonomous Region, Guilin Medical University, Guilin 541100, China"},{"name":"Key Laboratory of Medical Biotechnology and Translational Medicine, Education Department of Guangxi Zhuang Autonomous Region, Guilin Medical University, Guilin 541100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1021-1525","authenticated-orcid":false,"given":"Xian-Li","family":"Zhou","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Medical Engineering, Education Department of Guangxi Zhuang Autonomous Region, Guilin Medical University, Guilin 541100, China"},{"name":"Key Laboratory of Medical Biotechnology and Translational Medicine, Education Department of Guangxi Zhuang Autonomous Region, Guilin Medical University, Guilin 541100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,6]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (WHO) (2024, April 04). 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