{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T16:58:40Z","timestamp":1786640320330,"version":"build-2736575974"},"reference-count":37,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2025,6,24]],"date-time":"2025-06-24T00:00:00Z","timestamp":1750723200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The increasing environmental concerns and regulatory restrictions on toxic conventional solvents have driven the search for sustainable alternatives. Dimethyl isosorbide (DMI), a bio-renewable solvent, has shown potential as a replacement for short-chain glycol ethers, although its use as solvent in liquid chromatography (LC) is underexplored. This study presents a physicochemical characterization of DMI with a particular focus on its application as an innovative solvent in LC analyses. The partition coefficient (log P = \u22120.44) was determined using the OECD 107 method, and viscosity measurements for DMI and its mixtures with water and ethanol were conducted at 25 \u00b0C, 40 \u00b0C, and 60 \u00b0C. Viscosity ranged from 1.28 mPa\u00b7s at 60 \u00b0C to 2.62 mPa\u00b7s at 40 \u00b0C. The Central Composite Face 23 experimental design for studying the chromatographic behavior of DMI confirmed that 50% (v\/v) DMI can be effectively utilized in the mobile phases, at a column temperature of 40 \u00b0C, with backpressures ranging from 160 to 300 bar and a UV cut-off at 240 nm. Its effectiveness as an eluent in LC was demonstrated for the quantification of methylparaben and propylparaben in pharmaceutical formulations. This study highlights DMI\u2019s promise as a sustainable bio-renewable alternative to conventional organic solvents used as eluents in LC, supporting eco-friendly practices in pharmaceutical analysis.<\/jats:p>","DOI":"10.3390\/molecules30132713","type":"journal-article","created":{"date-parts":[[2025,6,24]],"date-time":"2025-06-24T06:49:38Z","timestamp":1750747778000},"page":"2713","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Dimethyl Isosorbide: An Innovative Bio-Renewable Solvent for Sustainable Chromatographic Applications"],"prefix":"10.3390","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-3248-0434","authenticated-orcid":false,"given":"Aleksandra","family":"Damjanoska","sequence":"first","affiliation":[{"name":"Institute of Applied Chemistry and Pharmaceutical Analysis, Faculty of Pharmacy, Ss Cyril and Methodius University in Skopje, Mother Tereza 47, 1000 Skopje, North Macedonia"},{"name":"Manufacturing Technical Operations, ALKALOID AD, Blvd. Aleksandar Makedonski 12, 1000 Skopje, North Macedonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristina","family":"Mitreska","sequence":"additional","affiliation":[{"name":"Institute of Applied Chemistry and Pharmaceutical Analysis, Faculty of Pharmacy, Ss Cyril and Methodius University in Skopje, Mother Tereza 47, 1000 Skopje, North Macedonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-8426-0658","authenticated-orcid":false,"given":"Matilda","family":"Petrova","sequence":"additional","affiliation":[{"name":"Manufacturing Technical Operations, ALKALOID AD, Blvd. Aleksandar Makedonski 12, 1000 Skopje, North Macedonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3887-5753","authenticated-orcid":false,"given":"Jelena","family":"Acevska","sequence":"additional","affiliation":[{"name":"Institute of Applied Chemistry and Pharmaceutical Analysis, Faculty of Pharmacy, Ss Cyril and Methodius University in Skopje, Mother Tereza 47, 1000 Skopje, North Macedonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4628-6986","authenticated-orcid":false,"given":"Katerina","family":"Brezovska","sequence":"additional","affiliation":[{"name":"Institute of Applied Chemistry and Pharmaceutical Analysis, Faculty of Pharmacy, Ss Cyril and Methodius University in Skopje, Mother Tereza 47, 1000 Skopje, North Macedonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2221-9850","authenticated-orcid":false,"given":"Natalija","family":"Nakov","sequence":"additional","affiliation":[{"name":"Institute of Applied Chemistry and Pharmaceutical Analysis, Faculty of Pharmacy, Ss Cyril and Methodius University in Skopje, Mother Tereza 47, 1000 Skopje, North Macedonia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3687","DOI":"10.1039\/C5GC00611B","article-title":"Perspectives on the Replacement of Harmful Organic Solvents in Analytical Methodologies: A Framework toward the Implementation of a Generation of Eco-Friendly Alternatives","volume":"17","author":"Kloskowski","year":"2015","journal-title":"Green Chem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.9734\/jpri\/2019\/v28i330203","article-title":"An Overview on Common Organic Solvents and Their Toxicity","volume":"28","author":"Joshi","year":"2019","journal-title":"J. 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