{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T17:31:19Z","timestamp":1779125479675,"version":"3.51.4"},"reference-count":59,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2025,6,24]],"date-time":"2025-06-24T00:00:00Z","timestamp":1750723200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Alzheimer\u2032s disease (AD) is a chronic neurodegenerative disorder that leads to memory loss and changes in mental and behavioral functions in elderly individuals. A major pathological feature of AD is the aggregation of amyloid-beta (A\u03b2) peptides, along with oxidative stress, inducing neurocellular apoptosis in the brain. Gobaishi (Galla chinensis), a traditional herbal medicine, has gained considerable attention for its constituents and potent therapeutic properties, particularly its strong inhibitory activity against A\u03b2 fibril formation. In this study, we investigated the anti-A\u03b2 aggregation effects of Gobaishi and its active constituents. We isolated two compounds by employing Thioflavin T (ThT) assay-guided fractionation, which were identified through various spectroscopic methods as pentagalloyl glucose (PGG) and methyl gallate (MG). Evaluation of their anti-A\u03b2 aggregation effects revealed that PGG and MG contribute 1.5% and 0.7% of the activity of Gobaishi, respectively. In addition, PGG demonstrated significantly stronger DPPH radical scavenging activity (EC50 = 1.16 \u00b5M) compared to MG (EC50 = 6.44 \u00b5M). At a concentration of 30 \u00b5M, PGG significantly reduced the A\u03b2-induced cytotoxicity in SH-SY5Y cell lines compared to MG. Based on these findings, both Gobaishi and its active compound PGG are proposed as promising candidates for further investigation as potent anti-amyloidogenic agents in AD management.<\/jats:p>","DOI":"10.3390\/molecules30132720","type":"journal-article","created":{"date-parts":[[2025,6,24]],"date-time":"2025-06-24T10:44:41Z","timestamp":1750761881000},"page":"2720","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Anti-Amyloid Aggregation Effects of Gobaishi (Galla chinensis) and Its Active Constituents"],"prefix":"10.3390","volume":"30","author":[{"given":"Sharmin","family":"Akter","sequence":"first","affiliation":[{"name":"Laboratory of Organic Chemistry in Life Science, Muroran Institute of Technology, Muroran 050-8585, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5555-5650","authenticated-orcid":false,"given":"Takayuki","family":"Tohge","sequence":"additional","affiliation":[{"name":"Laboratory of Plant Secondary Metabolism, Nara Institute of Science and Technology, Nara 630-0192, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sahithya Hulimane","family":"Ananda","sequence":"additional","affiliation":[{"name":"Laboratory of Protein Chemistry, Muroran Institute of Technology, Muroran 050-8585, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4958-1286","authenticated-orcid":false,"given":"Masahiro","family":"Kuragano","sequence":"additional","affiliation":[{"name":"Laboratory of Protein Chemistry, Muroran Institute of Technology, Muroran 050-8585, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7747-4808","authenticated-orcid":false,"given":"Kiyotaka","family":"Tokuraku","sequence":"additional","affiliation":[{"name":"Laboratory of Protein Chemistry, Muroran Institute of Technology, Muroran 050-8585, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1385-9001","authenticated-orcid":false,"given":"Koji","family":"Uwai","sequence":"additional","affiliation":[{"name":"Laboratory of Organic Chemistry in Life Science, Muroran Institute of Technology, Muroran 050-8585, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2216","DOI":"10.1007\/s12010-022-03937-1","article-title":"Isolation, characterization, and in silico interaction studies of bioactive compounds from Caesalpinia bonducella with target proteins involved in alzheimer\u2032s disease","volume":"195","author":"Khoba","year":"2023","journal-title":"Appl. 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