{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T12:41:30Z","timestamp":1783687290258,"version":"3.55.0"},"reference-count":33,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Three different vegetable oils, namely coconut oil, palm olein and olive oil, were analyzed for mineral oil hydrocarbons (MOHs) in our laboratory and in five commercial laboratories well recognized for their expertise in this field. The analysis consisted of a preliminary quantitative estimation of MOH content by hyphenated liquid chromatography\u2013gas chromatography with flame ionization detection (LC-GC-FID), followed by a confirmatory analysis of MOH components by two-dimensional gas chromatography with time-of-flight mass spectrometry (GCxGC-ToF). The results provided by the six laboratories were compared to check their consistency, which would have led to a hypothetical commercial agreement or dispute scenarios, for instance. The comparison was based merely on information provided by the laboratories in their analytical reports (i.e., the methodology was not challenged, and chromatograms were not reviewed). Additionally, some of the laboratories were willing to provide some more information or details of the analysis. Similar quantitative results were provided by all six laboratories, emphasizing the utility of the current available harmonized guidelines and official standards for this method. However, as regards confirmatory results, discrepancies were observed among some laboratories in terms of the detection of MOH markers at low levels and the interpretation of GCxGC-ToF information. Even taking into account the limitation of this study as regards the reduced number of laboratories included, it highlights the need for harmonizing the GCxGC-ToF confirmatory method for MOHs in order to increase the alignment of results between laboratories for this kind of analysis.<\/jats:p>","DOI":"10.3390\/molecules30132830","type":"journal-article","created":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T09:29:01Z","timestamp":1751362141000},"page":"2830","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Discrepancies in Mineral Oil Confirmation by Two-Dimensional Gas Chromatography\u2013Mass Spectrometry: A Call for Harmonization"],"prefix":"10.3390","volume":"30","author":[{"given":"Jos\u00e9 Fernando","family":"Huertas-P\u00e9rez","sequence":"first","affiliation":[{"name":"Nestl\u00e9 Research, Soci\u00e9t\u00e9 des Produits Nestl\u00e9 S.A., Vers-chez-les-Blanc, CH-1000 Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Cruz-Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Nestl\u00e9 Research, Soci\u00e9t\u00e9 des Produits Nestl\u00e9 S.A., Vers-chez-les-Blanc, CH-1000 Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"N\u00fa\u00f1ez-Galindo","sequence":"additional","affiliation":[{"name":"Nestl\u00e9 Research, Soci\u00e9t\u00e9 des Produits Nestl\u00e9 S.A., Vers-chez-les-Blanc, CH-1000 Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathieu","family":"Dubois","sequence":"additional","affiliation":[{"name":"Nestl\u00e9 Research, Soci\u00e9t\u00e9 des Produits Nestl\u00e9 S.A., Vers-chez-les-Blanc, CH-1000 Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lo\u00efc","family":"Perring","sequence":"additional","affiliation":[{"name":"Nestl\u00e9 Research, Soci\u00e9t\u00e9 des Produits Nestl\u00e9 S.A., Vers-chez-les-Blanc, CH-1000 Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrienne","family":"Tarres","sequence":"additional","affiliation":[{"name":"Nestl\u00e9 Research, Soci\u00e9t\u00e9 des Produits Nestl\u00e9 S.A., Vers-chez-les-Blanc, CH-1000 Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Nicolay","sequence":"additional","affiliation":[{"name":"Nestl\u00e9 Research, Soci\u00e9t\u00e9 des Produits Nestl\u00e9 S.A., Vers-chez-les-Blanc, CH-1000 Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9line","family":"Vocat","sequence":"additional","affiliation":[{"name":"Nestl\u00e9 Research, Soci\u00e9t\u00e9 des Produits Nestl\u00e9 S.A., Vers-chez-les-Blanc, CH-1000 Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1540-9645","authenticated-orcid":false,"given":"Thierry","family":"Delatour","sequence":"additional","affiliation":[{"name":"Nestl\u00e9 Research, Soci\u00e9t\u00e9 des Produits Nestl\u00e9 S.A., Vers-chez-les-Blanc, CH-1000 Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"5331","DOI":"10.1007\/s00216-018-1195-3","article-title":"Hits and misses in research trends to monitor contaminants in foods","volume":"410","author":"Lehotay","year":"2018","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1002\/ejlt.201400528","article-title":"Occurrence, determination, and assessment of mineral oils in oilseeds and vegetable oils","volume":"118","year":"2016","journal-title":"Eur. J. Lipid Sci. Technol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1845","DOI":"10.1080\/19440049.2018.1488185","article-title":"Mineral oil hydrocarbons in food: A review","volume":"35","author":"Grob","year":"2018","journal-title":"Food Addit. Contam.-Part A Chem. Anal. Control. Expo. Risk Assess."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1080\/10916466.2018.1427107","article-title":"Characterization and correlation of crude oils from some wells in the North Western Desert, Egypt","volume":"36","author":"Harb","year":"2018","journal-title":"Pet. Sci. Technol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1021\/acs.energyfuels.8b03268","article-title":"An Assay on Alkyl Aromatic Hydrocarbons, Unexpected Group-Type Separation of Diaromatic Hydrocarbons in Cretaceous Crude Oils from Brazilian Marginal Basin","volume":"33","author":"Moura","year":"2019","journal-title":"Energy Fuels"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"104252","DOI":"10.1016\/j.marpetgeo.2020.104252","article-title":"Influence of paleoenvironmental conditions on distribution and relative abundance of saturated and aromatic hydrocarbons in sediments from the NW part of the Toplica basin, Serbia","volume":"115","author":"Burazer","year":"2020","journal-title":"Mar. Pet. Geol."},{"key":"ref_7","unstructured":"Bratinova, S., Robouch, P., and Hoekstra, E. (2023). Guidance on Sampling, Analysis and Data Reporting for the Monitoring of Mineral Oil Hydrocarbons in Food and Food Contact Materials, Publications Office of the European Union. [2nd ed.]. JRC 133174."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.chroma.2012.05.095","article-title":"On-line coupled high performance liquid chromatography-gas chromatography for the analysis of contamination by mineral oil. Part 1, Method of analysis","volume":"1255","author":"Biedermann","year":"2012","journal-title":"J. Chromatogr. A"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.chroma.2012.05.096","article-title":"On-line coupled high performance liquid chromatography-gas chromatography for the analysis of contamination by mineral oil. Part 2, Migration from paperboard into dry foods, Interpretation of chromatograms","volume":"1255","author":"Biedermann","year":"2012","journal-title":"J. Chromatogr. A"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1177390118777757","DOI":"10.1177\/1177390118777757","article-title":"Analytical Methods for the Determination of Mineral Oil Saturated Hydrocarbons (MOSH) and Mineral Oil Aromatic Hydrocarbons (MOAH)\u2014A Short Review","volume":"13","author":"Weber","year":"2018","journal-title":"Anal. Chem. Insights"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1052","DOI":"10.1080\/19440049.2017.1306655","article-title":"Understanding the contamination of food with mineral oil: The need for a confirmatory analytical and procedural approach","volume":"34","author":"Spack","year":"2017","journal-title":"Food Addit. Contam.-Part A"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1080\/19440049.2019.1678770","article-title":"Mineral oil hydrocarbons in foods, Is the data reliable?","volume":"37","author":"Koster","year":"2020","journal-title":"Food Addit. Contam.-Part A Chem. Anal. Control. Expo. Risk Assess."},{"key":"ref_13","unstructured":"Bratinova, S., and Hoekstra, E. (2019). Guidance on Sampling, Analysis and Data Reporting for the Monitoring of Mineral Oil Hydrocarbons in Food and Food Contact Materials, Publications Office of the European Union. JRC115694."},{"key":"ref_14","unstructured":"(2002). European Commission Commission decision of 12 August 2002 implementing Council Directive 96\/23\/EC concerning the performance of analytical methods and the interpretation of the results (2002\/657\/EC). Off. J. Eur. Communities, L221, 8\u201336."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"EFSA Panel on Contaminants in the Food Chain (CONTAM) (2012). Scientific Opinion on Mineral Oil Hydrocarbons in Food. EFSA J., 10, 2704\u20132889.","DOI":"10.2903\/j.efsa.2012.2704"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.chroma.2004.05.071","article-title":"Comparison of conventional gas chromatography and comprehensive two-dimensional gas chromatography for the detailed analysis of petrochemical samples","volume":"1056","author":"Vendeuvre","year":"2004","journal-title":"J. Chromatogr. A"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"846","DOI":"10.1016\/j.orggeochem.2008.03.006","article-title":"Analysis of unresolved complex mixtures of hydrocarbons extracted from Late Archean sediments by comprehensive two-dimensional gas chromatography (GC\u00d7GC)","volume":"39","author":"Ventura","year":"2008","journal-title":"Org. Geochem."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"118890","DOI":"10.1016\/j.fuel.2020.118890","article-title":"Utility of comprehensive GC\u00d7GC-TOFMS in elucidation of aromatic hydrocarbon biomarkers","volume":"283","author":"Kumar","year":"2021","journal-title":"Fuel"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.chroma.2014.11.064","article-title":"Comprehensive two-dimensional gas chromatography for characterizing mineral oils in foods and distinguishing them from synthetic hydrocarbons","volume":"1375","author":"Biedermann","year":"2015","journal-title":"J. Chromatogr. A"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1080\/19440049.2017.1400185","article-title":"Comparability of mineral oil testing for dry food and cardboard samples\u2013Perspectives from different PT rounds","volume":"35","author":"Funk","year":"2018","journal-title":"Food Addit. Contam.-Part A Chem. Anal. Control. Expo. Risk Assess."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"10337","DOI":"10.1021\/acs.jafc.2c01189","article-title":"Determining MOSH and MOAH with High Sensitivity in Vegetable Oil\u2014A New, Reliable, and Comparable Approach Using Online LC-GC-FID\u2500Evaluation of Method Precision Data","volume":"70","author":"Albert","year":"2022","journal-title":"J. Agric. Food Chem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"461236","DOI":"10.1016\/j.chroma.2020.461236","article-title":"Epoxidation for the analysis of the mineral oil aromatic hydrocarbons in food. An update","volume":"1624","author":"Biedermann","year":"2020","journal-title":"J. Chromatogr. A"},{"key":"ref_23","unstructured":"(2017). Foodstuffs-Vegetable Oils and Foodstuff on Basis of Vegetable Oils-Determination of Mineral Oil Saturated Hydrocarbons (MOSH) and Mineral Oil Aromatic Hydrocarbons (MOAH) with Online HPLC-GC-FID Analysis (Standard No. EN 16995:2017)."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"462044","DOI":"10.1016\/j.chroma.2021.462044","article-title":"Mineral oil saturated and aromatic hydrocarbons quantification, Mono- and two-dimensional approaches","volume":"1643","author":"Bauwens","year":"2021","journal-title":"J. Chromatogr. A"},{"key":"ref_25","unstructured":"EFSA Panel on Contaminants in the Food Chain (CONTAM) (2023). Scientific Opinion-Update of the risk assessment of mineral oil hydrocarbons (MOH) in food. EFSA J., 21, 8215\u20138360."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"117671","DOI":"10.1016\/j.trac.2024.117671","article-title":"Views on the uses of comprehensive two-dimensional gas chromatography-mass spectrometry in food analysis over the literature period 2018\u20132023","volume":"174","author":"Tranchida","year":"2024","journal-title":"TrAC Trends Anal. Chem."},{"key":"ref_27","unstructured":"(2025, May 25). Method Ring Test MOSH\/MOAH in Chocolate by GCxGC-TOF P2101-MRT. Summary. Available online: https:\/\/www.proof-acs.de\/competenceschemes\/36\/summary."},{"key":"ref_28","unstructured":"(2025, May 25). Reference Material MOSH\/MOAH GCxGC-TOF Cheese (P2206-RMCh1). Summary. Available online: https:\/\/www.proof-acs.de\/upload\/media\/default\/0001\/01\/bbc15cd3f114ea1efa900bedece0047907753ecc.pdf."},{"key":"ref_29","unstructured":"(2024). Vegetable Oils\u2014Determination of Mineral Oil Saturated Hydrocarbons (MOSH) and Mineral Oil Aromatic Hydrocarbons (MOAH) with Online-Coupled High Performance Liquid Chromatography-Gas Chromatography-Flame Ionization Detection (HPLC-GC-FID) Analysis\u2014Method for Low Limit of Quantification (Standard No. ISO 20122:2024)."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"463523","DOI":"10.1016\/j.chroma.2022.463523","article-title":"Automated workflow utilizing saponification and improved epoxidation for the sensitive determination of mineral oil saturated and aromatic hydrocarbons in edible oils and fats","volume":"1682","author":"Nestola","year":"2022","journal-title":"J. Chromatogr. A"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"4478","DOI":"10.1016\/j.chroma.2011.05.009","article-title":"Comprehensive two-dimensional gas chromatography for the analysis of synthetic and crude-derived jet fuels","volume":"1218","author":"Ajam","year":"2011","journal-title":"J. Chromatogr. A"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1080\/10916466.2011.598896","article-title":"A Comparison of Normal and Reversed Phase Columns in Oil Analysis by Comprehensive Two-dimensional Gas Chromatography With Time-of-flight Mass Spectrometry","volume":"32","author":"Hu","year":"2014","journal-title":"Pet. Sci. Technol."},{"key":"ref_33","first-page":"11","article-title":"Advantages of comprehensive two-dimensional gas chromatography for comprehensive analysis of potential migrants from food contact materials","volume":"1057","author":"Biedermann","year":"2019","journal-title":"Anal. Chim. Acta"}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/30\/13\/2830\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:02:31Z","timestamp":1760032951000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/30\/13\/2830"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,1]]},"references-count":33,"journal-issue":{"issue":"13","published-online":{"date-parts":[[2025,7]]}},"alternative-id":["molecules30132830"],"URL":"https:\/\/doi.org\/10.3390\/molecules30132830","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,1]]}}}