{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T21:23:46Z","timestamp":1790976226305,"version":"4.1.0"},"reference-count":60,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T00:00:00Z","timestamp":1753747200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006378","name":"University of Indonesia","doi-asserted-by":"publisher","award":["NKB-170\/UN2.RST\/HKP.05.00\/2024"],"award-info":[{"award-number":["NKB-170\/UN2.RST\/HKP.05.00\/2024"]}],"id":[{"id":"10.13039\/501100006378","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Skin aging is a natural biological process that can be accelerated by free radical induction, leading to a reduction in skin elasticity and the formation of wrinkles due to the depletion of elastin. Eugenia uniflora (dewandaru) is a promising plant believed to possess anti-aging properties, primarily attributed to its major constituents, myricitrin and quercetin. This study aimed to investigate the anti-elastase and antioxidant properties of Eugenia uniflora stem bark, ripe fruit, and seed extracts. Extracts were obtained using an ultrasound-assisted extraction (UAE) method with 70% ethanol. Quantitative phytochemical analysis involved measuring the total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity. Bioactive constituents were identified using LC-MS analysis, and their interactions with target enzymes were further evaluated through in silico molecular docking. The results demonstrated that the E. uniflora seed extract exhibited the highest antioxidant activity, with an IC50 of 5.23 \u00b5g\/mL (DPPH assay) and a FRAP value of 3233.32 \u00b5mol FeSO4\/g. Furthermore, the ethanolic seed extract showed significant anti-elastase activity with an IC50 of 114.14 \u00b5g\/mL. Molecular docking predicted strong potential for several compounds as pancreatic elastase inhibitors, including 5-phenylvaleric acid, 2-(3-phenylpropyl)phenol, n-amylbenzene, 2-aminoadipic acid, and traumatin, each showing a prediction activity (PA) value exceeding 0.6. Notably, these compounds also exhibited inhibitory activity against tyrosinase. These findings collectively underscore the significant promise of E. uniflora seed extract as a novel and natural candidate for pharmacocosmeceutical product development, particularly for anti-aging applications.<\/jats:p>","DOI":"10.3390\/molecules30153168","type":"journal-article","created":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T12:39:29Z","timestamp":1753792769000},"page":"3168","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["In Vitro and In Silico Evaluation of the Anti-Aging Potential of Eugenia uniflora UAE Extracts"],"prefix":"10.3390","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5150-0000","authenticated-orcid":false,"given":"Desy Muliana","family":"Wenas","sequence":"first","affiliation":[{"name":"Faculty of Pharmacy, National Institute of Science and Technology, Jakarta 12630, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2904-6515","authenticated-orcid":false,"given":"Berna","family":"Elya","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Universitas Indonesia, Depok 16424, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8449-691X","authenticated-orcid":false,"given":"Sutriyo","family":"Sutriyo","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Universitas Indonesia, Depok 16424, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0170-9885","authenticated-orcid":false,"given":"Heri","family":"Setiawan","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Universitas Indonesia, Depok 16424, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8260-9252","authenticated-orcid":false,"given":"Rozana","family":"Othman","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universiti Malaya, Kuala Lumpur 50603, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7730-4414","authenticated-orcid":false,"given":"Syamsu","family":"Nur","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Almarisah Madani, Makassar 90245, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6123-2356","authenticated-orcid":false,"given":"Nita","family":"Triadisti","sequence":"additional","affiliation":[{"name":"Department of Biology Pharmacy, Faculty of Pharmacy, Universitas Muhammadiyah Banjarmasin, Banjarmasin 70115, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4595-1163","authenticated-orcid":false,"given":"Fenny","family":"Yunita","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Medicine, Tarumanagara University, Jakarta 11440, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9123-2303","authenticated-orcid":false,"given":"Erwi Putri","family":"Setyaningsih","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, National Institute of Science and Technology, Jakarta 12630, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,29]]},"reference":[{"key":"ref_1","first-page":"1054","article-title":"Bioactivities of Extracts from Eugenia uniflora L. 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