{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:36:52Z","timestamp":1760060212655,"version":"build-2065373602"},"reference-count":43,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2025,8,6]],"date-time":"2025-08-06T00:00:00Z","timestamp":1754438400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"JSPS KAKENHI","award":["JP22K06553"],"award-info":[{"award-number":["JP22K06553"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>This study focused on the poly(DL-lactide-co-glycolide)-block-poly(ethylene glycol)-block-poly(DL-lactide-co-glycolide) (PLGA-PEG-PLGA) triblock copolymer, which was recently reported as a novel material for polymeric nanoparticles to replace poly(DL-lactide-co-glycolide) (PLGA) as a drug carrier for prednisolone (PSL), and aimed to evaluate the efficacy of PSL-loaded PLGA-PEG-PLGA nanoparticles (NPs) against allergic contact dermatitis (ACD). PSL-loaded PLGA-PEG-PLGA NPs were prepared using the nanoprecipitation method, and their particle size distribution and mean particle size were measured using dynamic light scattering. 1-Fluoro-2,4-dinitrobenzene (DNFB) was used to create a mouse model of contact hypersensitivity (CHS). PSL-loaded PLGA-PEG-PLGA NPs were administered before sensitization with DNFB, and the therapeutic effect was evaluated by quantifying intracutaneous TNF-\u03b1 and IL-4 levels suing ELISA. When PSL-loaded PLGA-PEG-PLGA NPs were administered before sensitization, TNF-\u03b1 expression and IL-4 statements were significantly lower in the PSL-loaded PLGA-PEG-PLGA NP group than in the non-treated group. No significant difference was observed between the PSL-loaded PLGA-PEG-PLGA NP and PSL-loaded ointment groups, even though the steroid dose was 40 times lower than in the PSL-containing ointment. These results suggest that PSL-loaded PLGA-PEG-PLGA NPs may have a better effect in the treatment of ACD than PSL-loaded PLGA NPs.<\/jats:p>","DOI":"10.3390\/molecules30153292","type":"journal-article","created":{"date-parts":[[2025,8,6]],"date-time":"2025-08-06T10:13:51Z","timestamp":1754475231000},"page":"3292","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Therapeutic Effects of PSL-Loaded PLGA-PEG-PLGA NPs in Allergic Contact Dermatitis Model Mice"],"prefix":"10.3390","volume":"30","author":[{"given":"Ryo","family":"Fujisawa","sequence":"first","affiliation":[{"name":"Department of Medical Pharmacy, Graduate School of Pharmaceutical Sciences, Josai International University, 1 Gumyo Togane, Chiba 283-0002, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryuse","family":"Sakurai","sequence":"additional","affiliation":[{"name":"Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641, Yamazaki, Noda 278-8510, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-7324-8908","authenticated-orcid":false,"given":"Takeshi","family":"Oshizaka","sequence":"additional","affiliation":[{"name":"Department of Medical Pharmacy, Graduate School of Pharmaceutical Sciences, Josai International University, 1 Gumyo Togane, Chiba 283-0002, Japan"},{"name":"Faculty of Pharmaceutical Sciences, Josai International University, 1 Gumyo Togane, Chiba 283-0002, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenji","family":"Mori","sequence":"additional","affiliation":[{"name":"Department of Medical Pharmacy, Graduate School of Pharmaceutical Sciences, Josai International University, 1 Gumyo Togane, Chiba 283-0002, Japan"},{"name":"Faculty of Pharmaceutical Sciences, Josai International University, 1 Gumyo Togane, Chiba 283-0002, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8617-2441","authenticated-orcid":false,"given":"Akiyoshi","family":"Saitoh","sequence":"additional","affiliation":[{"name":"Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641, Yamazaki, Noda 278-8510, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0611-7387","authenticated-orcid":false,"given":"Issei","family":"Takeuchi","sequence":"additional","affiliation":[{"name":"Department of Medical Pharmacy, Graduate School of Pharmaceutical Sciences, Josai International University, 1 Gumyo Togane, Chiba 283-0002, Japan"},{"name":"Faculty of Pharmaceutical Sciences, Josai International University, 1 Gumyo Togane, Chiba 283-0002, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenji","family":"Sugibayashi","sequence":"additional","affiliation":[{"name":"Department of Medical Pharmacy, Graduate School of Pharmaceutical Sciences, Josai International University, 1 Gumyo Togane, Chiba 283-0002, Japan"},{"name":"Faculty of Pharmaceutical Sciences, Josai International University, 1 Gumyo Togane, Chiba 283-0002, Japan"},{"name":"Faculty of Pharmacy and Pharmaceutical Sciences, Josai University, 1-1 Keyakidai Sakado, Saitama 350-0295, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1261","DOI":"10.1038\/nbt.1504","article-title":"Transdermal drug delivery","volume":"26","author":"Mark","year":"2008","journal-title":"Nat. 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