{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T07:12:20Z","timestamp":1780557140664,"version":"3.54.1"},"reference-count":40,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2025,8,9]],"date-time":"2025-08-09T00:00:00Z","timestamp":1754697600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science Committee of the Ministry of Education and Science of the Republic of Kazakhstan","award":["AR19677062"],"award-info":[{"award-number":["AR19677062"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>As a continuation of our research on the synthesis and study of biological properties of new derivatives of 3-aminopyridin-2(1H)-ones, we investigated the Leuckart\u2013Wallach and Eschweiler\u2013Clarke reactions with selected 3-aminopyridin-2(1H)-ones and 3-(arylmethyl)pyridin-2(1H)-ones. It was found that under the conditions of the Leuckart\u2013Wallach reaction with aromatic aldehydes in formic acid, mainly formamides of the indicated 3-aminopyridones are formed. The Eschweiler\u2013Clarke reaction of 3-aminopyridin-2(1H)-ones and 3-(arylmethyl)pyridin-2(1H)-ones with an aqueous solution of formaldehyde result in the formation of tertiary N\u2013benzyl(methyl)amino)-pyridin-2(1H)-ones in almost quantitative yield. The 3-aminopyridin-2(1H)-ones derivatives synthesized by us were used for the biological screening of cytoprotective activity in the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) test to determine the viability of fibroblast cells isolated from the NIH\/Swiss mouse embryo (NIH\/3T3, Gibco). It was found that many of the studied compounds under the conditions of our experiment exhibited significant cytoprotective effects, thereby enhancing cell survival.<\/jats:p>","DOI":"10.3390\/molecules30163331","type":"journal-article","created":{"date-parts":[[2025,8,11]],"date-time":"2025-08-11T09:04:53Z","timestamp":1754903093000},"page":"3331","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Synthesis, Structure and Cytoprotective Activity of New Derivatives of 4-Aryl-3-Aminopyridin-2(1H)-One"],"prefix":"10.3390","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8148-0816","authenticated-orcid":false,"given":"Zarina","family":"Shulgau","sequence":"first","affiliation":[{"name":"National Laboratory Astana, Nazarbayev University, Kabanbai Batyr Ave. 53, Astana Z05H0P9, Kazakhstan"},{"name":"CF \u201cInstitute of Innovative and Preventive Medicine\u201d, Alikhan Bokeikhan Street, Building 1, Astana Z05M0M4, Kazakhstan"},{"name":"Department of Science Development, Astana Medical University, Beibitshilik St 49\/A, Astana Z10K9D9, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3376-6953","authenticated-orcid":false,"given":"Irina","family":"Palamarchuk","sequence":"additional","affiliation":[{"name":"School of Natural Sciences, Tyumen State University, 15a Perekopskaya St., Tyumen 625003, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Egor","family":"Dezhko","sequence":"additional","affiliation":[{"name":"School of Natural Sciences, Tyumen State University, 15a Perekopskaya St., Tyumen 625003, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6030-620X","authenticated-orcid":false,"given":"Shynggys","family":"Sergazy","sequence":"additional","affiliation":[{"name":"National Laboratory Astana, Nazarbayev University, Kabanbai Batyr Ave. 53, Astana Z05H0P9, Kazakhstan"},{"name":"CF \u201cInstitute of Innovative and Preventive Medicine\u201d, Alikhan Bokeikhan Street, Building 1, Astana Z05M0M4, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Assel","family":"Urazbayeva","sequence":"additional","affiliation":[{"name":"National Laboratory Astana, Nazarbayev University, Kabanbai Batyr Ave. 53, Astana Z05H0P9, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0695-0413","authenticated-orcid":false,"given":"Yuliya","family":"Safarova","sequence":"additional","affiliation":[{"name":"National Laboratory Astana, Nazarbayev University, Kabanbai Batyr Ave. 53, Astana Z05H0P9, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Gulyayev","sequence":"additional","affiliation":[{"name":"National Laboratory Astana, Nazarbayev University, Kabanbai Batyr Ave. 53, Astana Z05H0P9, Kazakhstan"},{"name":"CF \u201cInstitute of Innovative and Preventive Medicine\u201d, Alikhan Bokeikhan Street, Building 1, Astana Z05M0M4, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4128-7293","authenticated-orcid":false,"given":"Yuri","family":"Gatilov","sequence":"additional","affiliation":[{"name":"N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry, Siberian Branch of the Russian Academy of Science, 9 Akademika Lavrientieva Ave., Novosibirsk 630090, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5772-2096","authenticated-orcid":false,"given":"Ivan","family":"Kulakov","sequence":"additional","affiliation":[{"name":"National Laboratory Astana, Nazarbayev University, Kabanbai Batyr Ave. 53, Astana Z05H0P9, Kazakhstan"},{"name":"School of Natural Sciences, Tyumen State University, 15a Perekopskaya St., Tyumen 625003, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Benham\u00fa, B., Mart\u00edn-Fontecha, M., V\u00e1zquez-Villa, H., L\u00f3pez-Rodr\u00edguez, M.L., and Ortega-Guti\u00e9rrez, S. (2022). New trends in aging drug discovery. 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