{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T15:22:42Z","timestamp":1784733762832,"version":"3.55.0"},"reference-count":42,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2025,8,20]],"date-time":"2025-08-20T00:00:00Z","timestamp":1755648000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"\u201cPLAnt-based antiMIcrobial aNd circular PACKaging for plant products\u201d","award":["\u0393\u0393\u03a123-0562002\u2014\u0393\u0393\u0395\u039a Prima2023-06"],"award-info":[{"award-number":["\u0393\u0393\u03a123-0562002\u2014\u0393\u0393\u0395\u039a Prima2023-06"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>There is a growing interest in using essential oils with phytoprotectant properties instead of synthetic pesticides to mitigate the risks of insect pesticide resistance, environmental harm, and adverse effects on non-target organisms and human health. This study focused on the effects of Coridothymus capitatus essential oil on host selection, settling behaviour, and survival of Macrosiphoniella sanborni in dual-choice and no-choice tests. The essential oil and methanol extract of C. capitatus were analyzed using Gas Chromatography\u2013Mass Spectrometry (GC-MS) and Liquid Chromatography\u2013Mass Spectrometry (LTQ-LC-MS Orbitrap), respectively. The antioxidant activity was also tested through the radical scavenging assay. The settling inhibitory activity in the dual-choice test increased dose-dependently from 60% to 72% for essential oil concentrations of 0.1 to 0.3% (v\/v) for up to 120 min exposure, but decreased thereafter. However, under no-choice conditions, the inhibitory effect after 60 min of exposure was inversely proportional to the concentration but became proportional by the end of the experiment (72 h). After 72 h, both assays produced a mortality rate of 15% to 17%. C. capitatus was classified as a Carvacrol chemotype. Fifteen phenolic compounds were identified in the MeOH extract, and both the extract and essential oil exhibited substantial antioxidant activity. In conclusion, our findings indicate that C. capitatus essential oil affects the behaviour and survival of M. sanborni.<\/jats:p>","DOI":"10.3390\/molecules30163437","type":"journal-article","created":{"date-parts":[[2025,8,20]],"date-time":"2025-08-20T15:53:37Z","timestamp":1755705217000},"page":"3437","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Effect of Coridothymus capitatus Essential Oil on Chrysanthemum Aphid Behaviour and Survival: Phytochemical Analysis and Antioxidant Potential"],"prefix":"10.3390","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0202-7752","authenticated-orcid":false,"given":"Paraskevi","family":"Yfanti","sequence":"first","affiliation":[{"name":"Department of Agriculture, University of Ioannina, 47150 Arta, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Papavlasopoulos","sequence":"additional","affiliation":[{"name":"Independent Researcher, 45332 Ioannina, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Polyxeni","family":"Lazaridou","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dimitra","family":"Douma","sequence":"additional","affiliation":[{"name":"Independent Researcher, 45332 Ioannina, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8667-4927","authenticated-orcid":false,"given":"Marilena E.","family":"Lekka","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.1023\/A:1020881825669","article-title":"Essential oils as allelochemicals and their potential use as bioherbicides","volume":"25","author":"Dudai","year":"1999","journal-title":"J. Chem. Ecol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1146\/annurev-ento-120710-100554","article-title":"Essential oils in insect control: Low-risk products in a high-stakes world","volume":"57","author":"Vincent","year":"2012","journal-title":"Annu. Rev. Entomol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"6906105","DOI":"10.1155\/2018\/6906105","article-title":"Essential oils in stored product insect pest control","volume":"2018","author":"Campolo","year":"2018","journal-title":"J. Food Qual."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Sharifi-Rad, J., Sureda, A., Tenore, G.C., Daglia, M., Sharifi-Rad, M., Valussi, M., Tundis, R., Sharifi-Rad, M., Loizzo, M.R., and Ademiluyi, A.O. (2017). Biological activities of essential oils: From plant chemoecology to traditional healing systems. Molecules, 22.","DOI":"10.3390\/molecules22010070"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"354","DOI":"10.3923\/jest.2016.354.378","article-title":"Green pesticides: Essential oils as biopesticides in insect-pest management","volume":"9","author":"Mossa","year":"2016","journal-title":"Environ. Sci. Technol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Werrie, P.Y., Durenne, B., Delaplace, P., and Fauconnier, M.L. (2020). Phytotoxicity of essential oils: Opportunities and constraints for the development of biopesticides. A review. Foods, 9.","DOI":"10.3390\/foods9091291"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Kumar, S. (2019). Aphid-Plant Interactions: Implications for Pest Management. Plant Communities and Their Environment, IntechOpen.","DOI":"10.5772\/intechopen.84302"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1007\/s11829-024-10061-w","article-title":"Aphid honeydew in intraguild interactions: Enhancing predator mobility, foraging, and dynamics between Adalia bipunctata and Episyrphus balteatus","volume":"18","author":"Glacet","year":"2024","journal-title":"Arthropod-Plant Interact."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.plantsci.2018.04.027","article-title":"Plant defense against aphids, the pest extraordinaire","volume":"279","author":"Nalam","year":"2019","journal-title":"Plant Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"plz003","DOI":"10.1093\/aobpla\/plz003","article-title":"Herbivore-specific induction of indirect and direct defensive responses in leaves and roots","volume":"11","author":"Xiao","year":"2019","journal-title":"AoB PLANTS"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2786","DOI":"10.1007\/s13197-018-3203-1","article-title":"Chemical composition and antimicrobial activities of volatile oil extracted from Chrysanthemum morifolium Ramat","volume":"55","author":"Kuang","year":"2018","journal-title":"J. Food Sci. Technol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1270\/jsbbs.17075","article-title":"Molecular mechanisms underlying the diverse array of petal colors in chrysanthemum flowers","volume":"68","author":"Ohmiya","year":"2018","journal-title":"Breed. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2747","DOI":"10.1007\/s42690-021-00454-2","article-title":"Biological activity and safety evaluation of monoterpenes against the peach aphid (Myzus persicae Sulzer) (Hemiptera: Aphididae)","volume":"41","author":"Zhou","year":"2021","journal-title":"Int. J. Trop. Insect Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.cois.2019.01.001","article-title":"Progress and challenges in identifying molecular mechanisms underlying host and vector manipulation by plant viruses","volume":"33","author":"Mauck","year":"2019","journal-title":"Curr. Opin. Insect Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1038\/s41438-020-0334-0","article-title":"Chrysanthemum CmWRKY53 negatively regulates the resistance of chrysanthemum to the aphid Macrosiphoniella sanborni","volume":"7","author":"Zhang","year":"2020","journal-title":"Hortic. Res."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Benoutman, A., Erbiai, E.H., Edderdaki, F.Z., Cherif, E.K., Saidi, R., Lamrani, Z., Pintado, M., Pinto, E., da Silva, J.C.G.E., and Maouni, A. (2022). Phytochemical composition, antioxidant and antifungal Activity of Thymus capitatus, a medicinal plant collected from northern Morocco. Antibiotics, 11.","DOI":"10.3390\/antibiotics11050681"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2547169","DOI":"10.1155\/2016\/2547169","article-title":"Report on the medicinal use of eleven Lamiaceae species in Lebanon and rationalization of their antimicrobial potential by examination of the chemical composition and antimicrobial activity of their essential oils","volume":"2016","author":"Khoury","year":"2016","journal-title":"J. Evid. Based Complement. Altern. Med."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s11274-022-03363-9","article-title":"Assessment of the antioxidant, antimicrobial and antibiofilm activities of essential oils for potential application of active chitosan films in food preservation","volume":"38","author":"Mouhoub","year":"2022","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Castagna, A., Aboudia, A., Guendouz, A., Scieuzo, C., Falabella, P., Matthes, J., Schmid, M., Drissner, D., Allais, F., and Chadni, M. (2025). Transforming Agricultural Waste from Mediterranean Fruits into Renewable Materials and Products with a Circular and Digital Approach. Materials, 18.","DOI":"10.3390\/ma18071464"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Alves-Silva, J.M., Pedreiro, S., Cavaleiro, C., Cruz, M.T., Figueirinha, A., and Salgueiro, L. (2023). Effect of Thymbra capitata (L.) Cav. on inflammation, senescence and cell migration. Nutrients, 15.","DOI":"10.3390\/nu15081930"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Karpinski, T.M. (2020). Essential Oils of Lamiaceae Family Plants as Antifungals. Biomolecules, 10.","DOI":"10.3390\/biom10010103"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1080\/14786419.2014.984182","article-title":"Antifungal activity of chemically different essential oils from wild Tunisian thymus spp.","volume":"29","author":"Maissa","year":"2015","journal-title":"Nat. Prod. Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.foodchem.2007.03.059","article-title":"Chemical composition, antioxidant and antibacterial activities of the essential oils isolated from Tunisian Thymus capitatus Hoff. et Link","volume":"105","author":"Bounatirou","year":"2007","journal-title":"Food Chem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1500","DOI":"10.1089\/jmf.2009.0117","article-title":"Essential oil of Thymus capitatus Hoff. et Link. from Matmata, Tunisia: Gas chromatography-mass spectrometry analysis and antimicrobial and antioxidant activities","volume":"13","author":"Mkaddem","year":"2010","journal-title":"J. Med. Food"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"100288","DOI":"10.1016\/j.hermed.2019.100288","article-title":"Antiviral effect of an essential oil combination derived from three aromatic plants (Coridothymus capitatus (L.) Rchb. f., Origanum dictamnus L. and Salvia fruticosa Mill.) against viruses causing infections of the upper respiratory tract","volume":"17","author":"Tseliou","year":"2019","journal-title":"Herb. Med. J."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1021\/jf970822o","article-title":"Insecticidal and genotoxic activities of oregano essential oils","volume":"46","author":"Karpouhtsis","year":"1998","journal-title":"J. Agric. Food Chem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1002\/ps.548","article-title":"Activation of octopaminergic receptors by essential oil constituents isolated from aromatic plants: Possible mode of action against insect pests","volume":"58","author":"Kostyukovsky","year":"2002","journal-title":"Pest Manag. Sci. Former. Pestic. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1016\/j.indcrop.2010.10.021","article-title":"Variability in essential oil content and composition of Origanum hirtum L., Origanum onites L., Coridothymus capitatus (L.) and Satureja thymbra L. populations from the Greek island Ikaria","volume":"33","author":"Economou","year":"2011","journal-title":"Ind. Crops Prod."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.bse.2017.12.009","article-title":"Variation of phenolic constituents of Tunisian Thymus capitatus (L.) Hoff. et Link. populations","volume":"77","author":"Jaouadi","year":"2018","journal-title":"Biochem. Syst. Ecol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"48","DOI":"10.3390\/oxygen2020006","article-title":"Free radical properties, source and targets, antioxidant consumption and health","volume":"2","author":"Martemucci","year":"2022","journal-title":"Oxygen"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Yfanti, P., Lazaridou, P., Boti, V., Douma, D., and Lekka, M.E. (2024). Enrichment of olive oils with natural bioactive compounds from aromatic and medicinal herbs: Phytochemical analysis and antioxidant potential. Molecules, 29.","DOI":"10.3390\/molecules29051141"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.ifset.2017.08.017","article-title":"Nanoencapsulated Thymus capitatus essential oil as natural preservative","volume":"45","author":"Jemaa","year":"2018","journal-title":"Innov. Food Sci. Emerg. Technol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1122","DOI":"10.1002\/ps.1100","article-title":"Insecticidal activity of 23 essential oils and their major compounds against adult Lipaphis pseudobrassicae (Davis) (Aphididae: Homoptera)","volume":"61","author":"Sampson","year":"2005","journal-title":"Pest Manag. Sci. Former. Pestic. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1080\/10408398.2011.653458","article-title":"The bioactivity and toxicological actions of carvacrol","volume":"55","author":"Suntres","year":"2015","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_35","first-page":"1","article-title":"Carvacrol: An excellent natural pest control agent","volume":"6","author":"Bendre","year":"2018","journal-title":"Nat. Prod. Chem. Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"3861","DOI":"10.1021\/acsomega.2c06256","article-title":"Specificity of Monoterpene Interactions with Insect Octopamine and Tyramine Receptors: Insights from in Silico Sequence and Structure Comparison","volume":"8","author":"Ocampo","year":"2023","journal-title":"ACS Omega"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Jiang, H., Wang, J., Song, L., Cao, X., Yao, X., Tang, F., and Yue, Y. (2016). GC\u00d7 GC-TOFMS analysis of essential oils composition from leaves, twigs and seeds of Cinnamomum camphora L. Presl and their insecticidal and repellent activities. Molecules, 21.","DOI":"10.3390\/molecules21040423"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1111\/afe.12336","article-title":"Behavioural response of green peach aphid Myzus persicae (Sulzer) to volatiles from different rosemary (Rosmarinus officinalis L.) clones","volume":"21","author":"Dardouri","year":"2019","journal-title":"Agric. For. Entomol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1641","DOI":"10.1023\/B:JOEC.0000006428.00568.c5","article-title":"Behavioural and sublethal effects of structurally related lower terpenes on Myzus persicae","volume":"23","author":"Gutierrez","year":"1997","journal-title":"J. Chem. Ecol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1007\/s00217-017-3007-y","article-title":"Screening of Mushrooms Bioactivity: Piceatannol Was Identified as a Bioactive Ingredient in the Order Cantharellales","volume":"244","author":"Vasdekis","year":"2018","journal-title":"Eur. Food Res. Technol."},{"key":"ref_41","first-page":"1934578X211025165","article-title":"In vitro antifungal activity of Satureja horvatii ssp. macrophylla against three tomato phytopathogenic fungi","volume":"16","author":"Yfanti","year":"2021","journal-title":"Nat. Prod. Commun."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1093\/jee\/18.2.265a","article-title":"A method of computing the effectiveness of an insecticide","volume":"18","author":"Abbott","year":"1925","journal-title":"J. Econ. Entomol."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/30\/16\/3437\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:32:07Z","timestamp":1760034727000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/30\/16\/3437"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,20]]},"references-count":42,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2025,8]]}},"alternative-id":["molecules30163437"],"URL":"https:\/\/doi.org\/10.3390\/molecules30163437","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,20]]}}}